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Showing: 2321 - 2360 of 152514 results

  9. Desmoplakin I acceptor only control (F40-based tension sensor)

    Plasmid
    #119189
    Purpose
    The acceptor (mEYFP) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration or with the donor only control to determine intermolecular FRET.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1(Y72G)-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119189-plasmid-map-sequence-id-230531

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  10. Desmoplakin I donor only control (F40-based tension sensor)

    Plasmid
    #119188
    Purpose
    The donor (mTFP1) only control for the F40-based human desmoplakin I tension sensor can be used for bleedthrough calibration and provides the donor only lifetime to determine FRET efficiency.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I-[mTFP1-F40-mEYFP(Y67G)] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available since
    Dec. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    119188-plasmid-map-sequence-id-230532

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  14. pRRL-pEF-H2B-mCherry-T2A-rTetR-Dest-SV40

    Plasmid
    #186968
    Purpose
    Lentiviral destination vector for cloning desired protein as rTetR fusion for chromatin regulation reporter (pEF-H2B-mCherry-T2A-rTetR, with SV40) for expression in mammalian cells.
    Depositor
    Brian Liau
    Article
    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d
    Insert
    pEF-H2B-mCherry-T2A-rTetR-Dest-SV40
    Use
    Lentiviral
    Tags
    rTetR fusion
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    186968-plasmid-map-sequence-id-370475

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  15. Desmoplakin II donor only control (F40-based tension sensor)

    Plasmid
    #118717
    Purpose
    The donor (YPet(short)) only control for the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in fluorescence lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)-F40-mCherry(Y72L)] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118717-plasmid-map-sequence-id-230455

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  16. Desmoplakin II donor only control (F40-based no force control)

    Plasmid
    #118718
    Purpose
    The donor (YPet(short)) only control for the no force control of the F40-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-F40-mCherry(Y72L)] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y72L mutation in mCherry…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118718-plasmid-map-sequence-id-230460

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  19. Desmoplakin II acceptor only control (F40-based tension sensor)

    Plasmid
    #118719
    Purpose
    The acceptor (mCherry) only control for the F40-based human desmoplakin II tension sensor can be co-expressed with the donor (YPet(short)) only control to determine intermolecular FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II-[YPet(short)(Y67G)-F40-mCherry] (internal-1353) (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)(Y67G)-F40-mCherry
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118719-plasmid-map-sequence-id-230445

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  20. Desmoplakin II acceptor only control (F40-based no force control)

    Plasmid
    #118720
    Purpose
    The acceptor (mCherry) only control for the no force control of the F40-based human desmoplakin II tension sensor can be co-expressed with the donor only control to determine intermolecular FRET.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)(Y67G)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry(Y72L)
    Expression
    Mammalian
    Mutation
    truncation after aa1353; Y67G mutation in YPet(sh…
    Promoter
    CMV
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    118720-plasmid-map-sequence-id-230457

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  21. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid
    #69233
    Purpose
    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)
    Depositor
    Scot Wolfe
    Article
    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…
    Expression
    Mammalian
    Mutation
    Arginine 1335 is changed to Lysine
    Promoter
    Available since
    Nov. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    69233-plasmid-map-sequence-id-122312

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  23. pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

    Plasmid
    #172506
    Purpose
    Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning
    Depositor
    Omar Abudayyeh, Jonathan Gootenberg
    Article
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Insert
    DisCas7-11 bacterial expression vector with golden gate site for guides
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    172506-plasmid-map-sequence-id-344928

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  27. pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII

    Plasmid
    #192705
    Purpose
    Plasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728
    Depositor
    Sascha Martens
    Inserts
    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16L1
    Use
    Tags
    -GFP-TEV-StrepII and 10x-His-TEV
    Expression
    Insect
    Mutation
    F32A-I35A-I36A
    Promoter
    Available since
    Nov. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    192705-plasmid-map-sequence-id-377544

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  31. pDest17_Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene B protein LCD (1-136)

    Plasmid
    #235001
    Purpose
    Bacterial expression of N-terminally 6His tagged Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene B protein LCD
    Depositor
    Tanja Mittag
    Insert
    Beckwith-Wiedemann syndrome chromosomal region 1 candidate gene B protein (LOC129490717 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    235001-plasmid-map-sequence-id-466923

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Showing: 2321 - 2360 of 152514 results