We narrowed to 9,744 results for: tet
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Plasmid#129047Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA7 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA7 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-9
Plasmid#129049Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA9 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA9 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-10
Plasmid#129050Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA10 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA10 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-11
Plasmid#129051Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA11 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA11 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-12
Plasmid#129052Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA12 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA12 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-12
Plasmid#129040Purposetargeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA12 targeting human TSDRDepositorInsertdCas9-huTET1CD, SgRNA12 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-2
Plasmid#129042Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA2targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA2 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-3
Plasmid#129043Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA3 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA3 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-4
Plasmid#129044Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA4 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA4 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-5
Plasmid#129045Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA5 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA5 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-6
Plasmid#129046Purposeinactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA6 targeting human TSDRDepositorInsertdCas9-hudTET1CD, SgRNA6 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-5
Plasmid#129033Purposetargeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA5 targeting human TSDRDepositorInsertdCas9-huTET1CD, SgRNA5 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-6
Plasmid#129034Purposetargeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA6 targeting human TSDRDepositorInsertdCas9-huTET1CD, SgRNA6 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-7
Plasmid#129035Purposetargeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA7 targeting human TSDRDepositorInsertdCas9-huTET1CD, SgRNA7 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-9
Plasmid#129037Purposetargeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA9 targeting human TSDRDepositorInsertdCas9-huTET1CD, SgRNA9 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-10
Plasmid#129038Purposetargeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA10 targeting human TSDRDepositorInsertdCas9-huTET1CD, SgRNA10 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-11
Plasmid#129039Purposetargeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting human TSDRDepositorInsertdCas9-huTET1CD, SgRNA11 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2
Plasmid#129030Purposetargeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDRDepositorInsertdCas9-huTET1CD, SgRNA2 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-3
Plasmid#129031Purposetargeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting human TSDRDepositorInsertdCas9-huTET1CD, SgRNA3 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4
Plasmid#129032Purposetargeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDRDepositorInsertdCas9-huTET1CD, SgRNA4 (huTSDR)
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA
Plasmid#121803PurposepMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + EF1a::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA
Plasmid#121807PurposepMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.DepositorInsertpolyA + RIP::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-I894A(pL84)
Plasmid#124404PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.DepositorInsertGal4-TET1-878-911(I894A mutation) (TET1 Human)
Tagsgal4 dna binding domainExpressionMammalianMutationI894AAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-T898A(pL84)
Plasmid#124403PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.DepositorInsertGal4-TET1-878-911(T898A mutation) (TET1 Human)
Tagsgal4 dna binding domainExpressionMammalianMutationT898AAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-L900A(pL84)
Plasmid#124402PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.DepositorInsertGal4-TET1-878-911(L900A mutation) (TET1 Human)
Tagsgal4 dna binding domainExpressionMammalianMutationL900AAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-L897A(pL84)
Plasmid#124401PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.DepositorInsertGal4-TET1-878-911(L897A mutation) (TET1 Human)
Tagsgal4 dna binding domainExpressionMammalianMutationL897AAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA 24xMS2 SV40 CTE polyA
Plasmid#104097PurposeExpresses TREAT-PK co-localization control in mammalian cellsDepositorInsertRenilla luciferase
Tags24xMS2 stem loops in 3'UTR and 24xPP7 stem l…ExpressionMammalianAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron Renilla STOP 12xPP7 TNFα-ARE xrRNA12 24xMS2 SV40 CTE polyA
Plasmid#104096PurposeExpresses TREAT-ARE reporter in mammalian cellsDepositorInsertRenilla luciferase
Tags12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…ExpressionMammalianAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2-17
Plasmid#210020PurposetetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + super-Sox)DepositorUseLentiviralExpressionMammalianPromoterTetO-mini-CMVAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4
Plasmid#193345PurposetetO-OS*K (Dox-inducible reprogramming lentiviral OSK vector expressing mouse Oct4 + super-Sox + Klf4)DepositorUseLentiviralExpressionMammalianMutationSox2-17 is engineered highly cooperative chimeric…PromoterTetO mini CMVAvailable SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS
Plasmid#199349PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-SUMOEuExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-TetOn-ZIM3-KRAB-dCas9-P2A-mCherry
Plasmid#212829PurposeDoxycycline-inducible (Zim3)KRAB-dCas9-HA-P2A-mCherry cassette for integration at the AAVS1-locus of the human genome.DepositorInsertZIM3-KRAB-dCas9-P2A-mCherry
TagsHA-tag and ZIM3-KRABExpressionBacterial and MammalianPromoterCAG, TetOnAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]
Plasmid#227246PurposeAll-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cellDepositorInsertsTet-On 3G transactivator protein
Enhanced green fluorescent protein
UseCre/Lox and Synthetic BiologyExpressionMammalianPromoterEF1α and pTRE3GAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1
Plasmid#118593PurposeExpresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoterDepositorInsertPuro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
UseLentiviralExpressionMammalianPromoterEF1Alpha/TRE3GAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-DRD2-V2tail-TevN-BLITz1-TetR-VP16
Plasmid#89870Purposeexpresses DRD2-iTango component in mammalian cellsDepositorInsertDRD2-V2tail-TevN-BLITz1-TetR-VP16
ExpressionMammalianMutationshort isoform of DRD2 (deletion of 242-270)PromoterCMVAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP
Plasmid#199359PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOEu-EGFPExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)
Plasmid#70745Purposefor building 3-color reporter plasmidDepositorInsertmCherry-ssrA
Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)
Plasmid#166611PurposeEncodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TREDepositorInsertEGFP-2A-Tetanus Toxin
UseAAVPromotertetracycline response elementAvailable SinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Sin-Puro-Tet-RhoA FLARE.sc Biosensor
Plasmid#12153DepositorAvailable SinceJuly 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEx1-pEF-H2B-mCherry-T2A-rTetR-KRAB
Plasmid#78348PurposeExpresses rTetR-KRAB for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorInsertH2B-mCherry-T2A-rTetR-KRAB
UseSynthetic BiologyTagsrTetRExpressionMammalianPromoterpEF alphaAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only