We narrowed to 32,432 results for: Eng;
-
Plasmid#99225PurposeBacterial expression of the primed conversion capable protein pr-mEos4a (mEos4a-V69T).DepositorInsertpr-mEos4a
ExpressionBacterialMutationV69T for primed conversion applicationsPromoterT7Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
PatoG-OG241
Plasmid#72839PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.DepositorInsertPatoG
ExpressionBacterialPromoterPatoGAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
NG0911 pCI-TPI-SMG5(short)-4H
Plasmid#65805PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
Tags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianPromoterCMVAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDonr 201_MGA
Plasmid#240327PurposeEntry vector for Gateway with MGADepositorInsertMGA (MGA Human)
ExpressionBacterialAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDonr201_PARP1
Plasmid#240317PurposeEntry vector for Gateway with PARP1DepositorInsertPARP1 (PARP1 Human)
ExpressionBacterialAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSmBit-CHIP-G132N
Plasmid#236065PurposeSmBit-CHIP expression with G132N substitutionDepositorAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSmBit-CHIP-K30A
Plasmid#236066PurposeSmBit-CHIP expression with K30A substitutionDepositorAvailable SinceSept. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUS LC 8Y->S
Plasmid#226606Purpose8 tyrosines to serineDepositorInsertFUS LC 8Y->S (FUS Human)
Tags6HisExpressionBacterialMutation8 tyrosines to serinePromoterT7Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pILGFPEasy9R
Plasmid#218291PurposePlasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.DepositorInsertpURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Nova1-GFP
Plasmid#217033PurposeOverexpression of Nova in DRG neurons promotes the "mature" splicing pattern observed in CNS neuronsDepositorAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-Q2L7-T2176C-UGI-Puro
Plasmid#209648PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-Q2L7-T2176C
ExpressionMammalianAvailable SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-F14F15S-jGCaMP7s
Plasmid#178514PurposeAAV vector for Flpo recombinase dependent jGCaMP7sDepositorInsertjGCaMP7s
UseAAVExpressionMammalianAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-FZY2-S100N-UGI-Puro
Plasmid#209644PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-FZY2-S100N
ExpressionMammalianAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-MTS-G3635A-R16-FZY2-S100C-UGI-Puro
Plasmid#209643PurposeTo install m.G3635 mutation on mtDNADepositorInsertR16-FZY2-S100C
ExpressionMammalianAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM_mePOU_3xFlag
Plasmid#206395PurposeExpresses 3xflag fused mouse ePOU in mammalian cells, for lentivirus generation.DepositorInsertePOU
UseLentiviralTags3xFLAGExpressionMammalianAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM7
Plasmid#201213PurposeIntegrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.DepositorInsertssfGFP
Spo0A right flank
Spo0A left flank
UseSynthetic BiologyExpressionBacterialMutationF11V, T13P, N39D, A179AAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUBI-RZ-Cas12j2
Plasmid#189781PurposeCas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processingDepositorInsertgRNA cloning site
UseCRISPRAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIPKAH
Plasmid#185869PurposeKnocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)DepositorInsertGPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
ExpressionYeastMutationNoneAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTK-EdnrbE2-AVI-Tfap2B-tev-FLAG-2A-Citrine
Plasmid#127776PurposeModified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Tfap2B in frame with an Avi tag at the N terminus and FLAG-tag and Citrine at the C-terminusDepositorAvailable SinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
PatoK-OG241
Plasmid#72840PurposeNOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.DepositorInsertPatoK
ExpressionBacterialPromoterPatoKAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PatoL-LOG241
Plasmid#72846PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.DepositorInsertPatoL
ExpressionBacterialPromoterPatoLAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PatoK-LOG241
Plasmid#72848PurposeNOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.DepositorInsertPatoK
ExpressionBacterialPromoterPatoKAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS134-Pleum
Plasmid#60339PurposeContains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable SinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEXP5-NT-5th R
Plasmid#42572DepositorInsertmutated calmodulin gene
TagsPolyhistidine (6×His) region, TEV recognition sit…ExpressionBacterialMutationchanged Phenylalanine 92 to Glutamine acid, Isole…PromoterT7Available SinceMarch 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
MG1655 ΔendA ΔrecA (DE3)
Bacterial Strain#37854DepositorBacterial ResistanceNoneAvailable SinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-LIC_DDB1:1-1140_delta:396-689
Plasmid#210897PurposeInsect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.DepositorInsertDDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
ExpressionInsectMutationloop deletion amino acids I396 to D689PromoterPolyhedrinAvailable SinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBOH-avi-TEV-LIC_DCAF12:1-453
Plasmid#210940PurposeInsect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.DepositorInsertDCAF12:M1-S453 (DCAF12 Human)
ExpressionInsectMutationR131Q natural variant (VAR_035322)PromoterPolyhedrinAvailable SinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV_ENVLPE+
Plasmid#232427PurposeExpression plasmid to produce ENVLPE+ particlesDepositorInsertENVLPE+
ExpressionMammalianPromoter2xCMVAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_mGL_PP7
Plasmid#232440Purposeexpression plasmid to produce mGreenLantern mRNA with a PP7 aptamer in the 3' UTRDepositorInsertmGreenLantern
ExpressionMammalianAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_miniENVLPE
Plasmid#232431PurposeExpression plasmid to produce miniENVLPE particlesDepositorInsertminiENVLPE
ExpressionMammalianAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Flag-XBP1s
Plasmid#63680PurposeMammalian expression of Flag tagged XBP1sDepositorAvailable SinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GACh2.0
Plasmid#106073Purposeexpress the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cellsDepositorInsertGPCR activation based ACh sensor (GACh) 2.0
ExpressionMammalianPromoterCMVAvailable SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-fDIO-GFP-IRES-tTA
Plasmid#118026PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-fDIO-GFP-IRES-tTA
UseAAVMutationSee depositor comments belowPromoterTREAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pD3sE_SC12
Plasmid#219720PurposeDV3 E dimer with fusion loop mutation and increased expressionDepositorInsertDV3 sE SC12
Tags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…Available SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD4sE_SC12
Plasmid#219721PurposeDV4 stable E dimer with fusion loop mutation and increased expressionDepositorInsertDV4 sE SC12
Tags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A259W, S262R, F279W, A280P, G29K, T33V, A2…Available SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD1sE_SC12
Plasmid#219718PurposeDV1 E dimer with fusion loop mutation and increased expressionDepositorInsertDV1 sE SC12
Tags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A259W, T262R, F279W, A280P, S29K, T33V, A2…Available SinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD2sE_SC12
Plasmid#219719PurposeDV2 stable E dimer with fusion loop mutation and increased expressionDepositorInsertDV2 sE SC12
Tags8xHis and human serum albumin (HSA)ExpressionMammalianMutationG106D, A259W, T262R, F279W, T280P, S29K, T33V, A2…Available SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-hGSDMD
Plasmid#111559PurposeExpress human GSDMD in E.coliDepositorAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-Flpo
Plasmid#174378Purposealso known as: pAAV-synP-FLPo. AAV virus for expressing FLPo recombinaseDepositorHas ServiceAAV RetrogradeInsertFLPo recombinase
UseAAVAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-Flag-XBP1u
Plasmid#63679PurposeAdenoviral shuttle vector for XBP1u expressionDepositorInsertXBP1 (XBP1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationunsplicedPromoterCMVAvailable SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG eGFP NLS
Plasmid#170830PurposeDonor plasmid for knocking eGFP NLS into AAVS1 safe harbor locusDepositorInserteGFP
UseCRISPR, Synthetic Biology, and TALENTagsNLSExpressionMammalianPromoterCAGAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
iCAP-BI-28-KanR
Plasmid#203532PurposeRepCap for AAV productionDepositorInsertAAV-BI28 Cap
UseAAVExpressionMammalianAvailable SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAD100-CHIP-myc-His
Plasmid#236054PurposeMammalian expression of human CHIP with a C-terminal myc-His tagDepositorAvailable SinceAug. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-SC4
Plasmid#236254PurposeAAV-SC4 capsid which targets peripheral nerves in miceDepositorInsertsrep/cap AAV9 VP1 with GATPSTQ peptide insert
rep
UseAAVExpressionMammalianAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2
Plasmid#170823PurposePiggyBac Cas13d sgRNA plasmid for PPP2CA knockdownDepositorInsertCas13d PPP2CA gRNA2
UsePiggybac transposonExpressionMammalianPromoterU6Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Pyl-TAG
Plasmid#228784PurposePyl aaRS/tRNA system for mammalian TAG suppressionDepositorInsertsPylRS
PytR
UseBaculoviralExpressionMammalianMutationTAG suppressorAvailable SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-YE1-BE3-FNLS-CMV-mCherry
Plasmid#154005PurposeExpress YE1-BE3-FNLSDepositorInsertYE1-BE3-FNLS
UseCRISPRTagsFLAGPromoterCMVAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_rDA-mut
Plasmid#140558PurposeExpresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neuronsDepositorHas ServiceAAV9InsertRed fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
UseAAVPromoterhSynAvailable SinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-SUMO-mGSDMD
Plasmid#111560PurposeExpress mouse GSDMD in E.coliDepositorAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSP1101
Plasmid#65773PurposeHuman expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, G1218R, R1335E, and T1337R mutations in C…PromoterCMV & T7Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only