We narrowed to 14,413 results for: TIM

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Sox17(2A-phiC31o)

Plasmid

#140762

Purpose

Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Insert

phiC31o

Use

Mouse Targeting

Promoter

Endogenous Sox17

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(800-868)-strep

Plasmid

#180023

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868)-Strep.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(800-868)-Strep (PDCD6IP Human)

Tags

GB1-His6-TEV and Strep tag (WSHPQFEK)

Expression

Bacterial

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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shNg Lenti FHRC3J1UGW

Plasmid

#92233

Purpose

lentiviral expression of EGFP and Ng shRNA

Depositor

Weifeng Xu

Article

Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

Insert

shRNA targeting Ng

Use

Lentiviral

Expression

Mammalian

Promoter

H1

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pNHT7::RPL15::sNluc2

Plasmid

#186763

Purpose

An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Insert

Nanoluciferase

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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(B) HYG marker(R) (SBE264)

Plasmid

#195013

Purpose

hygromycin (HYG) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+HYG+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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(B) BLE marker(R) (SBE262)

Plasmid

#195012

Purpose

bleomycin (BLE) resistance genes codon-optimized for R. toruloides and expressed under pXYL and tGPD

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

pXYL+BLE+tGPD

Expression

Bacterial

Promoter

pXYL

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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ET1-human LRRC33

Plasmid

#111603

Purpose

Express human LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 (NRROS Human)

Tags

PreScission/TEV/ProteinC/6xHIS/SBP/

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs_SFXN1

Plasmid

#110635

Purpose

Retroviral expression of SFXN1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN1 (SFXN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

Plasmid

#127365

Purpose

Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Tags

GS linker, NLS, V5, and YFP

Expression

Plant

Mutation

R118G

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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CoV2-N-D377Y

Plasmid

#177957

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D377Y). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (D377Y) (N )

Expression

Mammalian

Mutation

D377Y

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLN189 (SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105209

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 2 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_dCas9-2xAM_hIRF-1

Plasmid

#92221

Purpose

Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Inserts

Sp-dCas9-2xAM tag
gRNA targeting human IRF-1 promoter

Use

CRISPR and Lentiviral

Tags

2xAM tag

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

CBh and U6

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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ath5:H2B-RFP

Plasmid

#105960

Purpose

transgenesis, appears rather late, likely due to long RFP maturation time but is brighter than most other Ath5 construct

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

H2B (H2BC21 Human)

Tags

RFP

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pKI-cjFOXL2-coemiRFP670-PGK-Puro

Plasmid

#186168

Purpose

Knock-in gene targeting in marmoset cells at FOXL2 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

emiRFP670

Use

Marmoset targeting

Mutation

human codon-optimized

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pVRa12_807

Plasmid

#49663

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF12_807

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pEGB2alpha2 35s:hCas9:tNos (GB0639)

Plasmid

#68222

Purpose

Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

hCas9

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGM55

Plasmid

#71249

Purpose

luciferase reporter with hGM-CSF -55 to +28 minimal promoter

Depositor

Peter Cockerill

Article

Bert et al Plasmid. 2000 Sep;44(2):173-82.

Insert

GM-CSF minimal promoter (-55 to +28) (CSF2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

GM-CSF minimal

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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PF3D7_0606800-bio

Plasmid

#47790

Purpose

Expresses enzymatically monobiotinylated full-length PF3D7_0606800 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised PF3D7_0606800

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pRSETa-mKikGR-V69T

Plasmid

#98574

Purpose

photoconvertible fluorescent protein mKikGR-V69T in pRSetA plasmid, photoconvertible by primed conversion mechanism, bacterial overexpression, sequence is codon optimised for E. coli

Depositor

Ulrike Endesfelder

Article

Turkowyd et al Angew Chem Int Ed Engl. 2017 Jun 2. doi:

Insert

mKikGR-V69T

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMM1157

Plasmid

#112534

Purpose

pZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
luxCDABE

Expression

Bacterial

Promoter

PL(HrtO) and ProD

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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