We narrowed to 10,466 results for: ESP

1. pIRESpuroN3xFLAG-His-CDK5RAP3

   Plasmid

   #204555

   Purpose

   Expresses 3xFLAG-His-tagged CDK5RAP3 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   CDK5RAP3 (AMY2A Human)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458 eSp(1.1) V3

   Plasmid

   #226963

   Purpose

   CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AW01_pU.CAG.eSpCas9(1.1).rBGpA

   Plasmid

   #199255

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1) nuclease

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

   Insert

   High specificity eSpCas9(1.1) nuclease
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   K848A K1003A R1060A

   Promoter

   CAG promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRESpuro-Flag-Pol b

   Plasmid

   #23257

   Depositor

   Robert Sobol

   Article

   Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

   Insert

   DNA polymerase beta (POLB Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 13, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pIRESpuroN3xFLAG-UFSP2 C302A

   Plasmid

   #204554

   Purpose

   Expresses 3xFLAG-tagged UFSP2 C302A in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   UFSP2 (UFSP2 Human)
   

   Expression

   Mammalian

   Mutation

   C302A

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

   Plasmid

   #86612

   Purpose

   Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via nhej using ouabain

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-R2L3-IRESpacDeltaTKpA

   Plasmid

   #139535

   Purpose

   Provides IRES-pacDeltaTK-pA cassette on Gateway entry vector

   Depositor

   Minoru Ko

   Insert

   PacTK
   

   Expression

   Mammalian

   Available Since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. eSpCas9(1.1)_No_FLAG_LMNA_G2

   Plasmid

   #178090

   Purpose

   Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. eSpCas9(1.1)_No_FLAG

   Plasmid

   #79877

   Purpose

   FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-deSpCas9

    Plasmid

    #92114

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. P823_eSpCas9(1.1)-RecA

    Plasmid

    #87264

    Purpose

    Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ki67 (LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183743

    Purpose

    Expresses Ki67 (LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain (MKI67 Human)
    

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    LR domain of Ki67

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pirespuro2-CD86-mEos2

    Plasmid

    #98284

    Purpose

    mammalian expression of CD86-mEos2

    Depositor

    Mike Heilemann

    Article

    Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

    Insert

    CD86 (CD86 Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP-Ki-67(RASA)-mCherry_IRESpuro2

    Plasmid

    #182921

    Purpose

    Expresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.

    Depositor

    Daniel Gerlich

    Article

    Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

    Insert

    MKI67 (MKI67 Human)
    

    Tags

    EGFP and mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    RASA mutation

    Promoter

    CMV

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. DNA-CARtcr-IRESpuro

    Plasmid

    #89346

    Purpose

    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
    

    Use

    Lentiviral

    Tags

    SNAPf tag

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. DNA-CARzeta-IRESpuro

    Plasmid

    #89345

    Purpose

    2nd generation lentiviral vector which expresses DNA-CAR receptor upstream of a IRES-puro cassette

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    DNA-CARzeta
    

    Use

    Lentiviral

    Tags

    SNAPf tag

    Expression

    Mammalian

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. mEmerald-Espin-like-C-18

    Plasmid

    #54086

    Purpose

    Localization: Actin Bundling , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Espin-like
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. BII-gR-BtW-eSpCas9

    Plasmid

    #133358

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Tags

    Flag-tagged eSpCas9

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIRESpuro2-UBXD1-mCherry

    Plasmid

    #31835

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    UBXD1 (UBXN6 Human)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

20. eSpCas9-EF-Gsk3b(new)

    Plasmid

    #122341

    Purpose

    Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Gsk3b
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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