We narrowed to 14,179 results for: cas9

1. hCas9

   Plasmid

   #41815

   Purpose

   Expresses human codon optimized Cas9 nuclease for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pdCas9

   Plasmid

   #46569

   Purpose

   Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   dcas9
   CRISPR array
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Reckleen_2_SpGCas9

   Plasmid

   #233457

   Purpose

   Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

   Depositor

   Anke Becker

   Article

   Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

   Insert

   Level 2 RECKLEEN plasmid
   

   Use

   CRISPR

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LentiV_Cas9_puro

   Plasmid

   #108100

   Purpose

   Lentiviral expression plasmid of spCas9 with puromycin resistance gene

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS promoter

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PB_tre_Cas9

   Plasmid

   #126029

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible Cas9

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   humanized S. pyrogenes Cas9
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Expression

   Mammalian

   Promoter

   EF1 alpha and TRE

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAS9counter

   Plasmid

   #107192

   Purpose

   Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   Cas9
   sgRNA
   

   Expression

   Bacterial

   Promoter

   prab17

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PB_tre_dCas9_VP160

   Plasmid

   #126031

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   dCas9 fusion with VP160
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive Cas9)

   Promoter

   EF1 alpha and TRE

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CAS9PBKS

   Plasmid

   #68371

   Purpose

   CbAkt promoter driven Cas9-2A-GFP (from px458)

   Depositor

   Eric-Paul Bennett

   Article

   Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

   Insert

   Cas9
   

   Tags

   2A-EGFP

   Expression

   Mammalian

   Promoter

   CbAkt

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LentiV_Cas9_Blast

    Plasmid

    #125592

    Purpose

    Lentiviral expression of spCas9 with blasticidin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS promoter

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MSCV_Cas9_puro

    Plasmid

    #65655

    Purpose

    Retroviral introduction of Cas9 into mammalian cell line.

    Depositor

    Christopher Vakoc

    Article

    Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

    Insert

    Cas9
    

    Use

    CRISPR and Retroviral

    Tags

    5 ' FLAG tag

    Expression

    Mammalian

    Promoter

    MSSV_LTR

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FUCas9Cherry

    Plasmid

    #70182

    Purpose

    Expresses Cas9 with fluorescent Cherry reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    humanised S.pyogenes cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Nme2Cas9_AAV

    Plasmid

    #119924

    Purpose

    All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    AAV

    Tags

    NLS-3xHA-NLS and NLS-NLS

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDual_dsCas9_Blast

    Plasmid

    #214681

    Purpose

    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites

    Depositor

    Kevin Janes

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NAX_CAGGS_CAS9_mcherry

    Plasmid

    #167862

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTi4.0_SpdCas9_pmcDA1

    Plasmid

    #204613

    Purpose

    CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NAX_CAGGS_CAS9_Puro

    Plasmid

    #167863

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSSBIO32_dCas9_RPA1786

    Plasmid

    #240489

    Purpose

    CRISPRi-mediated Knockdown of RPA1786

    Depositor

    Rajib Saha

    Article

    Kathol et al Appl Environ Microbiol. 2025 Aug 20;91(8

    Insert

    Cas9 (cas9 Streptococcus pyogenes)
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Plac

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAS9i

    Plasmid

    #141249

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hCas9_D10A

    Plasmid

    #41816

    Purpose

    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9_D10A
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A nickase

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge