We narrowed to 15,579 results for: grna

1. px458_2A_GFP_sgRNA_TIA1

   Plasmid

   #106097

   Purpose

   CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   gRNA TIA1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LCV2_mCherry_AAVS1_sgRNA_2

   Plasmid

   #155105

   Purpose

   lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p425_Cas9_gRNA_LEU_1014a

   Plasmid

   #87407

   Purpose

   p425_Cas9_gRNA-ARS1014a All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, LEU2 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ADH1, pTyrosine

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pegRNA2_tevopreQ1

   Plasmid

   #212039

   Purpose

   Bacterial expression of the pegRNA for genome editing in E. coli

   Depositor

   Quanjiang Ji

   Article

   Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

   Insert

   pegRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   J23119

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p102_gRNA_Sa_mClover

   Plasmid

   #213778

   Purpose

   Cloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   Sa gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p103_gRNA_Sp_mClover

   Plasmid

   #213779

   Purpose

   Cloning vector for Sp gRNA, contains Sp gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and GFP for mammalian screening.

   Depositor

   Howard Chang

   Article

   Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   Sp gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. sgRNA.SFFV.tBFP

   Plasmid

   #169940

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tBFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

   Depositor

   Benjamin Ebert

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmCherry_gRNA

   Plasmid

   #80457

   Purpose

   gRNA expression vector with mCherry transfection control

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Inserts

   sp Cas9 gRNA
   mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and chicken β-actin promoter

   Available since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EC2_4_dCas9_VPR_sgRNA

   Plasmid

   #163709

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VPR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pgRNA

    Plasmid

    #215560

    Purpose

    Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    gRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. gRNA_AAVS1-T1

    Plasmid

    #41817

    Purpose

    Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_AAVS1-T1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PtPuc3_diaCas9_sgRNA

    Plasmid

    #109219

    Purpose

    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

    Depositor

    Per Winge

    Article

    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

    Inserts

    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    LHCF1 terminator, LHCF2 promoter, U6 3' regi…

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSpCas9_U6_sgRNA_Mettl3C

    Plasmid

    #165420

    Purpose

    sgRNA construct for targeting Mettl3 C-terminus

    Depositor

    Neil Brockdorff

    Article

    Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

    Insert

    PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. sgRNA.SFFV.tRFP

    Plasmid

    #169941

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tRFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

    Depositor

    Benjamin Ebert

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. gRNA_GFP-T1

    Plasmid

    #41819

    Purpose

    Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_GFP-T1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EC2_3_dCas9_Mxi1_sgRNA

    Plasmid

    #163708

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    Mxi1+SV40 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. U6GRNA

    Plasmid

    #68370

    Purpose

    gRNA scaffold with hU6 promoter

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EC2_10_dCas9_VPR_HC_sgRNA

    Plasmid

    #163712

    Purpose

    Yeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domains

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VPR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. px458_2A_GFP_sgRNA_PELO

    Plasmid

    #127124

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA PELO (PELO Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. px458_2A_GFP_sgRNA_TIAL1

    Plasmid

    #106096

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIAL1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA TIAL1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge