We narrowed to 747 results for: streptococcus -(crispr) -(cas9)

1. Cas9 TLS2

   Plasmid

   #196978

   Purpose

   Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9 with TLS2 tag
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pG10-90

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Cas9 TLS1

   Plasmid

   #196977

   Purpose

   Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9 with TLS1 tag
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pG10-90

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Cas9 Null

   Plasmid

   #198727

   Purpose

   Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pG10-90

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCas9-CAT

   Plasmid

   #80323

   Purpose

   Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Inserts

   Cas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR

   Tags

   3X FLAG and NLS

   Expression

   Mutation

   Promoter

   TgTUB1

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHS26_10xHis_MBP_TEV_VRER-Cas9

   Plasmid

   #244829

   Purpose

   Bacterial expression of VRER Cas9 for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   VRER Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   D1135V/G1218R/R1335E/T1337R

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHS16_10xHis_MBP_TEV_xPBA-Cas9

   Plasmid

   #244830

   Purpose

   Bacterial expression of xPBA Cas9 for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   xPBA Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHS25_10xHis_MBP_TEV_EQR-Cas9

   Plasmid

   #244828

   Purpose

   Bacterial expression of EQR Cas9 for protein purification

   Depositor

   Jennifer Doudna

   Article

   Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

   Insert

   EQR Cas9
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   D1135E/R1335Q/T1337R

   Promoter

   T7

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EC2_2_dCas9_VP64_sgRNA

   Plasmid

   #163707

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VP64+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AB65_pCAG.Cas9-D10A.rBGpA

   Plasmid

   #199256

   Purpose

   Expression construct encoding the SpCas9-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   SpCas9.D10A nickase
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCas9-J23113

    Plasmid

    #119701

    Purpose

    Expression of Cas9 with a weak promoter

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJWV102-wtcas9sp

    Plasmid

    #99612

    Purpose

    Plasmid containing wild-type cas9sp

    Depositor

    Morten Kjos, Jan-Willem Veening

    Article

    van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

    Insert

    cas9sp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PczcD

    Available Since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

    Plasmid

    #101207

    Purpose

    Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N497A/R661A/Q695A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N497A, R661A and Q695A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

    Plasmid

    #101214

    Purpose

    Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant C80S/C574S/S355C/S867C/K855A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    C80S, C574S, S355C, S867C and K855A

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOSIP-KH-RBS2-dCas9

    Plasmid

    #182740

    Purpose

    Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

    Depositor

    David Bikard

    Article

    Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

    Insert

    dCas9
    

    Use

    CRISPR; Integrative vector

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HiFi dCas9 VPR

    Plasmid

    #188510

    Purpose

    Expresses FLAG tagged HiFi dCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/R691A/H840A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HiFi dCas9 KRAB

    Plasmid

    #188500

    Purpose

    Expresses FLAG tagged HiFi dCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/R691A/H840A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

    Plasmid

    #86987

    Purpose

    For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

    Depositor

    Ralf Kuehn

    Article

    Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV_CK8e_Cas9_miniPA

    Plasmid

    #226141

    Purpose

    AAV construct for HITI insert with CK8e promoter and SpCas9

    Depositor

    Christopher Nelson

    Article

    Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

    Insert

    Cas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CK8e

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Plasmid

    #101232

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G528A/V583A/E584A/D585A/N588A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G528A, V583A, E584A, D585A, N588A and Q926A

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104911

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge