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We narrowed to 59,462 results for: plasmid

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Showing: 1181 - 1200 of 59462 results

  1. MSP1101

    Plasmid
    #65773
    Purpose
    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, and T1337R mutations in C…
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65773-plasmid-map-sequence-id-112657

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  2. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid
    #60367
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNase HI-D10R-E48R
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60367-plasmid-map-sequence-id-456413

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  6. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50454
    Purpose
    Double floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has service
    AAV8
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50454-plasmid-map-sequence-id-214300

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  10. AAV-CamKIIa-jGCaMP8m-WPRE

    Plasmid
    #176751
    Purpose
    AAV-mediated expression of GCaMP8m under the CamKIIa promoter
    Depositor
    Loren Looger
    Has service
    AAV1, AAV5, AAV9, and AAV Retrograde
    Insert
    jGCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Promoter
    CamKIIalpha
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    176751-plasmid-map-sequence-id-349058

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  13. pENN.AAV.CMVs.Pl.Cre.rBG (AAV2)

    Viral prep
    #105537-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pENN.AAV.CMVs.Pl.Cre.rBG (#105537). In addition to the viral particles, you will also receive purified pENN.AAV.CMVs.Pl.Cre.rBG plasmid DNA. CMV-driven Cre. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. pFC334

    Plasmid
    #87846
    Purpose
    AMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymes
    Depositor
    Uffe Mortensen
    Publication
    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do
    Inserts
    Cas9
    argB
    yA specific sgRNA
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    A. nidulans gpdA promoter with Hammerhead ribozym…
    Available since
    May 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87846-plasmid-map-sequence-id-174613

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  16. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid
    #60365
    Purpose
    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNase HI
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60365-plasmid-map-sequence-id-256737

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  19. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid
    #70684
    Purpose
    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.
    Depositor
    Bob Goldstein
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70684-plasmid-map-sequence-id-306412

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  20. pAAV-CKIIa-stGtACR2-FusionRed (AAV Retrograde)

    Viral prep
    #105669-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-CKIIa-stGtACR2-FusionRed (#105669). In addition to the viral particles, you will also receive purified pAAV-CKIIa-stGtACR2-FusionRed plasmid DNA. CaMKIIa-driven, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Publication
    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1
    Promoter
    CaMKII
    Tags
    FusionRed
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1181 - 1200 of 59462 results