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Plasmid#38313DepositorInsertGas1 (GAS1 Budding Yeast)
UseTags2xHAExpressionMutationcontains promoter regionPromoterEndogenousAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFTK004
Plasmid#171276PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPgpdA ANIA_08041 promoter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pREDusk-StrR-MCS
Plasmid#188972PurposeFor red light-repressed bacterial expression of gene of interest (StrR)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK036
Plasmid#171308PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertamdS ANIA_08777 acetamidase, selection marker
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rha
Plasmid#129390PurposeDerived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.DepositorInsertRhamnose-inducible promoter
UseTagsExpressionBacterialMutationN/APromoterPrhaBADAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbS15k-RFP
Plasmid#126194PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23100Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUdO4t
Plasmid#210249Purposelow-copy plasmid for bacterial expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-PheB
Plasmid#71738PurposeModular shuttle vector for Geobacillus and E. coliDepositorInsertPheB
UseSynthetic Biology; Shuttle vectorTagsExpressionBacterialMutationPromoterpRplsWTAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbA19a-RFP
Plasmid#126101PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23105Available sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMZ2007
Plasmid#204399PurposeA 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsquorum sensing regulator LuxR
GFP
UseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PLux0002Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-slr0230-PL22-sgRNANT1-KmR
Plasmid#73224PurposeContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coliDepositorInsertPL22-sgRNA NT1
UseTagsExpressionBacterialMutationPromoterPL22Available sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pICH44022
Plasmid#50330DepositorInsertCDS, P19 suppressor of gene silencing (Tomato Bushy Stunt Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL50010
Plasmid#50310DepositorInsertC terminal Myc tag (4x Myc)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL13001
Plasmid#50265DepositorInsertpromoter (1.3 kb) + 5'UTR, 35s (Cauliflower Mosaic Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJBL7026
Plasmid#128811PurposeFluoride riboswitch-regulated 3-way junction dimeric broccoliDepositorInsertFluoride riboswitch-regulated 3-way junction dimeric broccoli
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJin-278
Plasmid#125714PurposeABA-inducible split T7 GFP vector using RNAPN d5-19DepositorInsertPT7-GFP mRNA expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-ccdB
Plasmid#99915PurposeBasic cloning vector for the expression of imaging-based optimization sgRNADepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM731
Plasmid#68896PurposeIPTG-inducible CRISPRi vector targeting NanoLuc (NL1), constiutive NanoLuc, pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
NanoLuc
UseTagsExpressionMutationPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-V220A
Plasmid#47645PurposepAC-EsaR with a c659t mutation in esaRDepositorInsertesaR-V220A
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Valine 220 to AlaninePromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-ChE1 v4.5
Plasmid#185755PurposeBacterial expression of ChE1 v4.5DepositorInsertChE1 v4.5
UseTagsHis10ExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
CDS_aadA_chlo
Plasmid#163935PurposeL0 part - CDSDepositorInsertaadA gene for spectinomycin resistance
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pILGFP4M
Plasmid#104508PurposeUsed to evaluate the expression output of Saccharomyces eubayanus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSeGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-guideless
Plasmid#129464PurposeDerived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.DepositorInsertGuideless gRNA backbone
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTK043
Plasmid#171315PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInserteGFP fluorescent reporter
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSecEffector
Plasmid#172891PurposeContains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901DepositorInsertsBxb1
TP901
TEV Display cassette
ProD promoter between anti aligned Bxb1 sites
UseTagsExpressionBacterialMutationPromoterProD, nTETO, and pBAD /AraCAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL80007
Plasmid#50323DepositorInsertCDS, mCherry variant of RFP (Discosoma sp.)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHES841
Plasmid#89194PurposeExpresses YFP under control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-YFP
UseTagsExpressionYeastMutationPromoterpZAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR
Plasmid#60619PurposecrRNA expression plasmid on pRS423 backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL5
Plasmid#72989PurposeRBS_M2DepositorInsertM2 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBP-TL11
Plasmid#72995PurposeRBS_L4DepositorInsertL4 RBS
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BASIC_8_RBS34- mCherry -B0015
Plasmid#68142Purposestorage vector for BASIC part: RBS-RFP(ORF)DepositorInsertRBS34-mcherry-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19, containing H2-Kb c57/BL6 WK9
Plasmid#102641PurposeEncodes sequence of the murine H2-Kb alleleDepositorInsertH2-Kd Balb/c
UseTagsExpressionMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+pG10-90
Plasmid#170879PurposePhytobrick - pG10-90DepositorInsertpG10-90
UseTagsExpressionPlantMutationPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHES874
Plasmid#89196PurposeExpresses mCherry under control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-mCherry
UseTagsExpressionYeastMutationPromoterpZAvailable sinceOct. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICSL50007
Plasmid#50308DepositorInsertC terminal FLAG tag
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
BASIC_7_J23101-RBS34-mCherry-B0015
Plasmid#68141Purposestorage vector for BASIC part: RFP expression cassetteDepositorInsertJ23101-RBS34-mcherry-B0015
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
MyrPalm-BKAR
Plasmid#14878DepositorInsertB kinase activity reporter
UseTagsCFP, Myr/Palm, and YFPExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
NAT-TET-OFF -LEU- 4xFLAG
Plasmid#182527PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsert7 Copies of TetO Box
UseTags4XFLAGExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH75111
Plasmid#50327DepositorInsertCDS, beta-glucuronidase (E. coli) with two introns,
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Split-Broccoli-Toehold-Switch
Plasmid#87316PurposeExpresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.DepositorInsertSplit-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only