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Showing: 3001 - 3050 of 9524 results

  3. pGBW-m4046831

    Plasmid
    #145671
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145671-plasmid-map-sequence-id-319574

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  4. pGBW-m4046970

    Plasmid
    #145753
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145753-plasmid-map-sequence-id-311539

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  9. pCEP4-SERTI425V-myc

    Plasmid
    #107464
    Purpose
    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425V conferring aproximately wildtype phenotype
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Serotonin Transporter (SERT) (SLC6A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    I425V
    Promoter
    CMV
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    107464-plasmid-map-sequence-id-205573

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  27. pcDNA3-Cyto-ExRai-CKAR2

    Plasmid
    #236097
    Purpose
    Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.
    Depositor
    Jin Zhang
    Article
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Insert
    ExRai-CKAR2-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    236097-plasmid-map-sequence-id-473746

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  28. pcDNA3-PM-ExRai-CKAR2

    Plasmid
    #236098
    Purpose
    Plasma membrane-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.
    Depositor
    Jin Zhang
    Article
    Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038
    Insert
    Lyn-ExRai-CKAR2
    Use
    Tags
    N-terminal targeting sequence from Lyn kinase
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236098-plasmid-map-sequence-id-473738

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  38. pCAGEN_N73_eMagBF_V5

    Plasmid
    #235642
    Purpose
    Encodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5. No nuclear export sequence to allow nuclear localization.
    Depositor
    Maria Barna
    Article
    Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.
    Insert
    ALIBi N-terminal fragment
    Use
    Tags
    eMagBF-V5
    Expression
    Mammalian
    Mutation
    residues 1-73
    Promoter
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    235642-plasmid-map-sequence-id-467581

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  48. pOPINF HopF2

    Plasmid
    #228397
    Purpose
    Recombinant expression of His6-tagged Pseudomonas syringae pv tomato type III effector HopF2 in E. coli
    Depositor
    Lennart Wirthmueller
    Article
    Simranjit et al bioRxiv 2023.09.25.558804
    Insert
    HopF2 (hopF2 Pseudomonas syringae pv. tomato)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    March 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228397-plasmid-map-sequence-id-465273

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  50. pLANT-2b

    Plasmid
    #231487
    Purpose
    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.
    Depositor
    Venkat Gopalan
    Inserts
    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    Use
    Tags
    His6 tag and His6-SUMO^NIT
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…
    Promoter
    T7 promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231487-plasmid-map-sequence-id-460673

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Showing: 3001 - 3050 of 9524 results