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pmGFP-ADAR2

Plasmid

#117929

Purpose

ADAR2 expression plasmid.

Depositor

Kumiko Ui-Tei

Article

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Insert

ADAR2 (ADARB1 Human)

Tags

myc-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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mEGFP-Rac1

Plasmid

#83950

Purpose

Rac1 FLIM donor for dynamic imaging of GTPase activity

Depositor

Ryohei Yasuda

Article

Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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PA-Cdc42

Plasmid

#75263

Purpose

Light-activated Cdc42

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

Cell Division Cycle 42 (CDC42 Human)

Tags

6xHIs, LOV2, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

F56W

Promoter

CMV

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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p35S-GW-3Avi

Plasmid

#127918

Purpose

Gateway destination vector for transcient expression of proteins with C-terminal 3_Avi in plant protoplast

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Type

Empty backbone

Tags

3xAviTag

Expression

Plant

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBirA-P19

Plasmid

#127919

Purpose

Transient expression of biotin ligase BirA and p19 in plant protoplast

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

BirA

Expression

Plant

Promoter

35S

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSCIP-mB2M

Plasmid

#154091

Purpose

Self-Cutting and Integrating CRISPR backbone targeting murine B2M

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA and BAIT targeting murine B2M locus

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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DEP-GFP

Plasmid

#109043

Purpose

Expression of the Dishevelled DEP domain

Depositor

Mariann Bienz

Article

Gammons et al Mol Cell. 2016 Oct 6;64(1):92-104. doi:

Insert

dishevelled segment polarity protein 2 (DVL2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Dvl2 DEP domain

Promoter

CMV

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pQCi-hTRAC-sgRNA

Plasmid

#154093

Purpose

pQCi backbone with sgRNA targeting human TCR-alpha common chain

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA targeting human TRAC locus

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSCIP-hTRAC

Plasmid

#154094

Purpose

Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA and BAIT targeting human TRAC locus

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPG-GPD-TRPC

Plasmid

#190113

Purpose

Expression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selection

Depositor

Perng-Kuang Chang

Article

Chang et al Fungal Genet Biol. 2017 Jul;104:29-37. d

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ADAR2DD_E488Q

Plasmid

#205598

Purpose

expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

ADAR2 deaminase domain (ADARB1 Human)

Expression

Mammalian

Mutation

E488Q hyperactive mutation

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET30a-Tn5

Plasmid

#127917

Purpose

Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

Tn5

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSCIP-hCD69-TdTomato

Plasmid

#154095

Purpose

Self-Cutting and Integrating CRISPR backbone targeting human CD69 promoter with TdTomato reporter transgene

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Inserts

[5HA]-P2A-tdTomato-[3HA]
hCD69 targeting sgRNA - AGCTCTTTGCATCCGGAGAG

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Bax1-2/L-6

Plasmid

#30533

Depositor

Richard Youle

Article

Edlich et al Cell. 2011 Apr 1. 145(1):104-16.

Insert

BCL2-associated X protein (BAX Human)

Tags

eGFP

Expression

Mammalian

Mutation

F30C E44C C62S L63C C126S P130C

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pHAGE Flag HA ADAR2 CI

Plasmid

#237997

Purpose

Inducible lentiviral expression of ADAR2 CI

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

ADAR2 (ADARB1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

E396A

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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RCAS(A) dRhoA V14 (CT#846)

Plasmid

#13949

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

RhoA (Rho1 Fly)

Use

Retroviral; Avian expression

Mutation

G14V mutation

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
ADARB1_pCSdest

Plasmid

#53939

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ADARB1 (ADARB1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
RCAS(A) dRhoA N19 (CT#847)

Plasmid

#14002

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

RhoA (Rho1 Fly)

Use

Retroviral; Avian expression

Mutation

T19N

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pET14b-mEKLF

Plasmid

#67834

Purpose

Encodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitution

Depositor

James Bieker

Article

Armstrong et al Cell. 1998 Oct 2;95(1):93-104.

Insert

EKLF (Klf1 Mouse)

Expression

Bacterial

Mutation

Full-length mEKLF is aa 20-376; amino acid 19 is …

Promoter

T7

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pSicoR (Puro) NEMO shRNA1

Plasmid

#22505

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

NEMO shRNA1 (Ikbkg Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only