We narrowed to 8,961 results for: chia
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Plasmid#17820DepositorInsertMevT
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
taRAJ31min
Plasmid#69934PurposeExpresses taRAJ31min, a linear riboregulator.DepositorInsertplTet-taRAJ31min
UseSynthetic BiologyTagsExpressionMutationPromoterPltetO1-2Available sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEDuet40
Plasmid#46378DepositorInsertTvoVMA intein
UseTagsGB1 and H6-MBPExpressionBacterialMutationPromoterT7Available sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLY034b
Plasmid#227936PurposeComplementary plasmid providing gIIIDepositorInsertgIII (recoded)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS10-ZBASIC-TEV-GG
Plasmid#215797PurposeCloning of target gene with N-terminal His10 Zbasic tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC- AVITAG-CTH10STII- -GG
Plasmid#215805PurposeCloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDSG546
Plasmid#214212PurposeArabinose-inducible folA (used in cross-feeding experiment)DepositorInsertAraC, folA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG589
Plasmid#214217Purposecompetition integrase RBS library parent plasmid (myc-tagged)DepositorInsertPhlF, A118
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG553
Plasmid#214214Purposeprecut biphasic target plasmid version 1.0DepositorInsertAraC, trpC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG588
Plasmid#214216Purposebiphasic target plasmid version 2.0 ("flip-proof")DepositorInsertfolA, trpC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG545
Plasmid#214211Purposebiphasic target plasmid version 1.0DepositorInsertAraC, folA, trpC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG550
Plasmid#214213PurposeArabinose-inducible trpC (used in cross-feeding experiment)DepositorInsertAraC, trpC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP22
Plasmid#212855PurposeExpresses the Scytonema sp. HK-05 LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationPromoterPcymRCAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLRD25
Plasmid#208272PurposeProduction of cis-abienol in E. coli MEV20DepositorInsertstHMGR
abCAS
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKP1411
Plasmid#167638PurposeExpress FepA I14C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA I14C
UseTagsExpressionBacterialMutationfepA with I14C mutation (Mutation is I36C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1383
Plasmid#167639PurposeExpress FepA V15C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA V15C
UseTagsExpressionBacterialMutationfepA with V15C mutation (Mutation is V37C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1384
Plasmid#167641PurposeExpress FepA V16C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA V16C
UseTagsExpressionBacterialMutationfepA with V16C mutation (Mutation is V38C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1416
Plasmid#167643PurposeExpress FepA T17C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA T17C
UseTagsExpressionBacterialMutationfepA with T17C mutation (Mutation is T39C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1583
Plasmid#167645PurposeExpress FepA D34C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA D34C
UseTagsExpressionBacterialMutationfepA with D34C mutation (Mutation is D56C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1382
Plasmid#167647PurposeExpress FepA G54C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA G54C
UseTagsExpressionBacterialMutationfepA with G54C mutation (Mutation is G76C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2164
Plasmid#167608PurposeExpress ExbD V45A, L49ADepositorInsertexbD V45A, L49A
UseTagsExpressionBacterialMutationexbD with V45A, L49A mutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2171
Plasmid#167609PurposeExpress ExbD V45A, V47ADepositorInsertexbD V45A, V47A
UseTagsExpressionBacterialMutationexbD with V45A, V47A mutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2182
Plasmid#167610PurposeExpress ExbD V45A,P50ADepositorInsertexbD V45A, P50A
UseTagsExpressionBacterialMutationexbD with V45A, P50A mutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2190
Plasmid#167612PurposeExpress ExbD V47A, P50ADepositorInsertexbD V47A, P50A
UseTagsExpressionBacterialMutationexbD with V47A, P50A mutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2202
Plasmid#167613PurposeExpress ExbD V49A, P50ADepositorInsertexbD V49A, P50A
UseTagsExpressionBacterialMutationexbD with V49A, LP50A mutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2304
Plasmid#167633PurposeExpress TonB C18G, Q160C, F202A, W213A.DepositorInserttonB C18G, Q160C, F202A, W213A
UseTagsExpressionBacterialMutationtonB with C18G, Q160C, F202A, W213A mutations. Pr…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP718
Plasmid#167636PurposeExpress FepA T13C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA T13C
UseTagsExpressionBacterialMutationfepA with T13C mutation (Mutation is T35C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2201
Plasmid#167611PurposeExpress ExbD V47A, L49ADepositorInsertexbD V47A, L49A
UseTagsExpressionBacterialMutationexbD with V47A, L49A mutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2032
Plasmid#167651PurposeExpress His6-ExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229ADepositorInserthis6-exbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S101A, T111A, S112A, S221A, S229A
UseTagsExpressionBacterialMutationExbB S43A, S45A, S67A, S80A, S82A, S84A, S98A, S1…PromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1410
Plasmid#167634PurposeExpress FepA D12C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA D12C
UseTagsExpressionBacterialMutationFepA with D12C mutation. (Numbering based on star…PromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP920
Plasmid#160814PurposeExpression of ExbB proteinDepositorInsertexbB
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTAT6
Plasmid#112594PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag-thioredoxin fusionsDepositorTypeEmpty backboneUseTags6xHis-thioredoxinExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTAT5
Plasmid#112593PurposeUsed for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-thioredoxin fusionsDepositorTypeEmpty backboneUseTags6xHis-thioredoxinExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
c1GFP-W53XKir7.1
Plasmid#107183PurposeGenerates Nonsense mutant cloneDepositorInsertKCNJ13 (KCNJ13 Human)
UseTagsEGFPExpressionMammalianMutationW53XPromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterNative promoter from pMUT2 relB/relE operon and P…Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-Flag-c-Myc
Plasmid#102625PurposeExpresses c-Myc in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
DYRK1A/pTRE3G-FL-hXIST
Plasmid#149608PurposeExpress doxycycline-inducible full length of human XIST cDNADepositorInsertX-inactive specific transcript (XIST Human)
UseTagsExpressionMammalianMutationPromoterpTRE3GAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUG-Pten
Plasmid#140732PurposeLentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion proteinDepositorInsertGFP-Pten (PTEN Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterhUbiCAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
p168-759
Plasmid#62619PurposeePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)DepositorInsertsflavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
UseSynthetic BiologyTagsGBD, PDZ, and SH3ExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
araBAD-BCD20-ClpP-BCD17-ClpX
Plasmid#153301PurposeExpresses clpX and clpP in E.ColiDepositorUseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-2
Plasmid#72677PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterpL promoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSP6-RNAP
Plasmid#48143PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylADepositorInsertrna polymerase SP6
UseSynthetic BiologyTagsExpressionBacterialMutationV314A (numbering based on NCBI Sequence ID: NP_85…PromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_CEACAM6-His
Plasmid#149337Purposefor eukaryotic expression and purification of CEACAM6-His6DepositorInsertCEACAM6 (CEACAM6 Human)
UseTagsIL-2 signal peptide and polyhistidineExpressionMammalianMutationpoint mutation (GGA to GCA) to replace 'G-g…PromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF16B tail 458-1317
Plasmid#134624PurposeMammalian expression of human KIF16B 458-1317DepositorInsertKIF16B (KIF16B Human)
UseTagseGFPExpressionMammalianMutationWT with deletion of the 457 N-terminal residues.PromoterChicken Beta actinAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6410
Plasmid#47049PurposeBglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6XHis-SUMO-SIRT2
Plasmid#102622PurposeExpresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purificationDepositorInsertSIRT2 (SIRT2 Human)
UseTags6XHis-SUMOExpressionBacterialMutationDeleted amino acids 1-37 and 357-389PromoterAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_cRed
Plasmid#117225PurposepET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.DepositorInsertCanthaxanthin Operon
UseTags34 amino acid N-terminal linker, Hexahistidine Ta…ExpressionBacterialMutationremoved Type IIS restriction sites within the bac…PromoterT7 RNA PolymeraseAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only