We narrowed to 1,335 results for: piggyBac

1. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #190033

   Purpose

   vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13X
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y672A,Y676A

   Promoter

   CBh, SV40, hU6

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #191797

   Purpose

   vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized dCas13d
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   CBh, SV40, hU6

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-tetO-hNIL

   Plasmid

   #197089

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR

   Plasmid

   #213480

   Purpose

   AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   mtagBFP-KASH
   U6-gRNA-directcapture
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTR01F/hFGFR2c-V5

   Plasmid

   #236013

   Purpose

   for PiggyBac mediated integration and stable expression of hFGFR2c protein

   Depositor

   Pavel Krejčí

   Article

   Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

   Insert

   human FGFR2c (FGFR2 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   truncated CMV promoter

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTR01F/hFGFR2b-V5

   Plasmid

   #236014

   Purpose

   for PiggyBac mediated integration and stable expression of hFGFR2b protein

   Depositor

   Pavel Krejčí

   Article

   Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

   Insert

   human FGFR2b (FGFR2 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   truncated CMV promoter

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

   Plasmid

   #210523

   Purpose

   AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   GFP-KASH
   U6-gRNA-directcapture
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-AMBRA1-3xFLAG

   Plasmid

   #174159

   Purpose

   PiggyBac vector to stably express 3xFLAG-tagged AMBRA1

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA1 (AMBRA1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTR01F/hFGFR1b-V5

   Plasmid

   #236012

   Purpose

   for PiggyBac mediated integration and stable expression of hFGFR1b protein

   Depositor

   Pavel Krejčí

   Article

   Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

   Insert

   human FGFR1b (FGFR1 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   truncated CMV promoter

   Available Since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-PG13-mCherry-EF1a-PuroR-EGFP

    Plasmid

    #195292

    Purpose

    piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

    Depositor

    Hideyuki Oguro

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    mCherry
    PuroR-P2A-EGFP
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid

    #191796

    Purpose

    vector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

    Insert

    humanized dCas13X
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    R84A, H89A, R739A, R740A, H745A, H746A, H747A

    Promoter

    CBh, SV40, hU6

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-AMBRA1-501*-3xFLAG

    Plasmid

    #174160

    Purpose

    PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Missing aa 501-1208

    Promoter

    EF1A

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-AMBRA1-217*-3xFLAG

    Plasmid

    #174161

    Purpose

    PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Missing aa 217-1208

    Promoter

    EF1A

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-NLS-ZapCV2

    Plasmid

    #203664

    Purpose

    Expresses zinc sensor ZapCV2 in the nucleus of mammalian cells

    Depositor

    Amy Palmer

    Article

    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

    Insert

    ZapCV2 Zinc sensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. OA-986B

    Plasmid

    #124999

    Purpose

    Expresses dCas9-VPR driven by the Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VPR
    

    Use

    Piggyback

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. OA-986C

    Plasmid

    #125000

    Purpose

    Expresses dCas9-VPR driven by the bottleneck promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VPR
    

    Use

    Piggyback

    Tags

    Expression

    Insect

    Mutation

    Promoter

    bottleneck

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OA-986D

    Plasmid

    #125001

    Purpose

    Expresses dCas9-VP64 driven by the Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VP64
    

    Use

    Piggyback

    Tags

    Expression

    Insect

    Mutation

    Promoter

    ubiquitin

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OA-986E

    Plasmid

    #125002

    Purpose

    Expresses dCas9-VP64 driven by the bottleneck promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VP64
    

    Use

    Piggyback

    Tags

    Expression

    Insect

    Mutation

    Promoter

    bottleneck

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-NES-ZapCV2-Zinc dead

    Plasmid

    #203850

    Purpose

    Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

    Depositor

    Amy Palmer

    Article

    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

    Insert

    ZapCV2-Ala zinc dead sensor
    

    Use

    Tags

    Nuclear Export Signal

    Expression

    Mammalian

    Mutation

    Cys and His in Zap1 is mutated with Ala

    Promoter

    CMV promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-NLS-ZapCV2-ALA

    Plasmid

    #203665

    Purpose

    Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells

    Depositor

    Amy Palmer

    Article

    Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

    Insert

    ZapCV2-Ala zinc dead sensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Cys and His in Zap1 is mutated with Ala

    Promoter

    CMV promoter

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge