We narrowed to 5,560 results for: pcas

1. pBT225_(pCA-tdT3Myc)

   Plasmid

   #36873

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Insert

   tdTomato-3Myc
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer)

   Available since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-SRGAP3FBAR-EGFP

   Plasmid

   #168506

   Purpose

   Expression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusion

   Depositor

   Franck Polleux

   Article

   Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

   Insert

   SRGAP3-FBAR (SRGAP3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa 1-492

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_CALR sgRNA / hSpCas9

   Plasmid

   #172838

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-AmCas12b-2AeGFP

   Plasmid

   #121947

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AmCas12b
   

   Use

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-Intron4-PAFAH1B1

   Plasmid

   #92434

   Purpose

   expresses Cas9 and gRNA targeting PAFAH1B1

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

   Insert

   PAFAH1B1 (PAFAH1B1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-TetR-RNF169UBD

   Plasmid

   #119011

   Purpose

   Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   TetR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA24N-ligase-pprA

   Plasmid

   #87744

   Purpose

   IPTG-inducible expression of ligase-pprA fusion for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis and codon optimized pprA (from Deinococcus …

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCaSpeR-act5cB-LexAQFw

   Plasmid

   #61311

   Purpose

   Expresses chimeric transactivator LexA_DBD:QF2w_AD in Drosophila

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   LexA_DBD::QF2w_AD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Changed EK on C-terminus of QF2 to KKKK

   Promoter

   Available since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCALNL-BsaI-EGFP

   Plasmid

   #123136

   Purpose

   Golden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.

   Depositor

   David Liu

   Article

   Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

   Insert

   EGFP, mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cBA

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG aconitase GFP

    Plasmid

    #66104

    Purpose

    expression of chicken aconitase GFP

    Depositor

    Martin Scaal, Felicitas Proels

    Article

    et al Development. 2015 Feb 15;142(4):665-71.

    Insert

    aconitase
    

    Use

    Chicken expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pET-FLAG-eSpCas9

    Plasmid

    #126769

    Purpose

    Expression of increased fidelity eSpCas9 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCaSpeR3/ncd-gfp #10

    Plasmid

    #66082

    Purpose

    stable germline transformation of Drosophila to produce Ncd-GFP expression

    Depositor

    Sharyn Endow

    Article

    Endow et al J Cell Biol. 1997 Jun 16;137(6):1321-36.

    Insert

    Ncd kinesin-14 motor protein (ncd Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    D699L

    Promoter

    ncd promoter (3.1kb upstream region of ncd start …

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pCAGGS-TAL-NC2

    Plasmid

    #43856

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

    Insert

    TALEN-FokI nuclease backbone
    

    Use

    Tags

    Flag and FokI nuclease

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-HDAC4 1-129

    Plasmid

    #71401

    Purpose

    CAG promoter, mammalian expression

    Depositor

    Bo Chen

    Article

    Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

    Insert

    HDAC4 1-129 (HDAC4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pBT255_(pCA-GFP4m)

    Plasmid

    #36877

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F64L; S65T; V163A; S175G

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer)

    Available since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCALNL-eGFP-Esp3I

    Plasmid

    #81226

    Purpose

    Cloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sites

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    Esp31-Neo-Esp31 deletion cassette upstream of EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCBa

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-HDAC4-IGd2

    Plasmid

    #22536

    Purpose

    To be used for the RNAi screening purposes (not for HDAC4 expression); see paper

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    Histone Deacetylase 4 (HDAC4 Human)
    

    Use

    Tags

    IGd2 = Ires-destabilized GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pCAG-p21_T145A/S146A

    Plasmid

    #42623

    Depositor

    Philipp Mergenthaler

    Article

    Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T145A/S146A

    Promoter

    CAG

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. CpcBfull-NaGLS fusion_CpcA

    Plasmid

    #74004

    Purpose

    Expresses the Nicotiana attenuata GLS as a fusion with the CpcB protein plus the CmR cassete in Synechocystis

    Depositor

    Anastasios Melis

    Article

    Formighieri et al Planta. 2018 Oct;248(4):933-946. doi: 10

    Insert

    NaGLS and CmR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cpc

    Available since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-△MTS-TFAM-V5

    Plasmid

    #200934

    Purpose

    expresses △MTS-TFAM-V5 in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    Transcription Factor A, Mitochondrial (TFAM Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    deleted mitochondrial targeting sequence (MTS)

    Promoter

    CAG

    Available since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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