We narrowed to 5,945 results for: pcas

1. pCAG-eGtACR1-mRuby2-ST

   Plasmid

   #109136

   Purpose

   Anion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   eGtACR1-mRuby2-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-Chronos-mRuby2-ST

   Plasmid

   #105446

   Purpose

   Cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

   Insert

   Chronos-mRuby2-ST
   

   Tags

   mRuby2 and soma targeting motif

   Promoter

   pCAGGS

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas9-beta-mmr

   Plasmid

   #169241

   Purpose

   Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   mutL-E36K
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Mutation

   Changed glutamic acid 36 to lysine

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAS9-mCherry-ACTG1

   Plasmid

   #66943

   Purpose

   CRISPaint target selector ACTG1

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA ACTG1
   

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-PxCas12a-2AeGFP

   Plasmid

   #123637

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   PxCas12a
   

   Tags

   3xHA tag

   Expression

   Mammalian

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-PHFF-sh-mBVRA

   Plasmid

   #100286

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

   Inserts

   MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
   shRNA for mouse BVRA
   

   Tags

   FLAG, T7 and Mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   All genes are codon-optimized for expression in h…

   Promoter

   CAG promoter and H1 promoter

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_ACTB-i4 sgRNA / hSpCas9

   Plasmid

   #172828

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-S-B.1.617.2-FLAG

   Plasmid

   #177097

   Purpose

   B.1.617.2 variant S-protein with c-terminal FLAG tag

   Depositor

   Daniel Conway

   Insert

   S-protein B.1.617.2 (S SARS-CoV-2)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 2, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCAG-Dcst1-3xFLAG

   Plasmid

   #183542

   Purpose

   Expression vector of mouse DC-STAMP domain containing 1 (Dcst1) tagged with 3xFLAG at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

   Insert

   DC-STAMP domain containing 1 (Dcst1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-AkCas12b-2AeGFP

    Plasmid

    #121946

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AkCas12b
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL3-B-264;+80pCalb2

    Plasmid

    #66746

    Purpose

    luciferase reporter for Calb2 promoter (-264 to +80, WT)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-264bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Calb2

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-B-419;+80pCalb2

    Plasmid

    #66747

    Purpose

    luciferase reporter for Calb2 promoter (-419 to +80, WT)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-419bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Calb2

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-nDsRedFL-miR124

    Plasmid

    #177806

    Purpose

    The pre-miR-124 sequence has been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-124
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-GCaMP2-actin

    Plasmid

    #18928

    Depositor

    Karel Svoboda

    Article

    Mao et al PLoS ONE. 2008 . 3(3):e1796.

    Insert

    GCaMP2-Actin (ACTB Chicken)
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

15. BII-gR-BtW-eSpCas9

    Plasmid

    #133358

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Tags

    Flag-tagged eSpCas9

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-AviMta2-3xFiBsd

    Plasmid

    #140964

    Purpose

    full-length wt Avi-Mta2-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

    Depositor

    Brian Hendrich

    Article

    Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

    Insert

    metastasis-associated gene family, member 2 (Mta2 Mouse)
    

    Tags

    3xFLAG, Avi, and TEV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCaSpeR-act5cB-GAL4QF

    Plasmid

    #61309

    Purpose

    Expresses chimeric transactivator GAL4_DBD:QF2w_AD in Drosophila

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    GAL4_DBD::QF2w_AD
    

    Expression

    Insect

    Mutation

    Changed EK on C-terminus of QF2 to KKKK

    Available Since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. G1397 DddAtox-C–dSpCas9

    Plasmid

    #157836

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
    

    Use

    CRISPR

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9–mKate2ps–CgRNA

    Plasmid

    #64955

    Purpose

    Control gRNA (GTCAAGGCACTCTTGCCTA)

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Expression

    Mammalian

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-HA-NLGN4 D396*

    Plasmid

    #59321

    Purpose

    Expression plasmid of HA-tagged human Neuroligin 4 harboring a D396* nonsense mutation

    Depositor

    Peter Scheiffele

    Article

    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

    Insert

    NLGN4 D396* (NLGN4X Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D396* nonsense mutation, rest of ORF deleted

    Promoter

    CAG

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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