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pME-mScarlet3-Giantin (JDW 1356)

Plasmid

#224502

Purpose

Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-Giantin

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pME V5-mTagBFP2 (JDW 830)

Plasmid

#224484

Purpose

Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mTagBFP2

Use

Gateway cloning

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pET30a-SARS-CoV2-NP-Nt(47-173)

Plasmid

#165091

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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p5E-hs_ICAM_full (JDW 1047)

Plasmid

#224479

Purpose

Gateway compatible 5' entry clone containing the Human ICAM2 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

ICAM2 promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS

Plasmid

#117384

Purpose

An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET32a-Spike-S-4P

Plasmid

#165094

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)

Expression

Bacterial

Available Since

March 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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p3E-V5-mScarlet-I_SV40pA (JDW 968)

Plasmid

#224529

Purpose

A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

V5-mScarlet-I

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET30a-SARS-CoV2-ORF3b (S23Q)

Plasmid

#165088

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)

Expression

Bacterial

Mutation

Stop codon to glutamine at 23rd amino acid

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pT2/GD-IRES-GFP-CTNNB1

Plasmid

#192868

Purpose

Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

Depositor

David Largaespada

Article

Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

Inserts

luc+
EGFP
CTNNB1

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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R725-X09-635: His6-tev-Hs.SPRED1(13-125)

Plasmid

#159581

Purpose

Baculovirus protein expression of His6-tev-Hs.SPRED1(13-125)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-tev-Hs.SPRED1(13-125) (SPRED1 Human)

Tags

His6-tev

Expression

Insect

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-HtrA1-PDZ-1

Plasmid

#250091

Purpose

Bacterial expression of HtrA1 PDZ domain 1

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

HtrA1 (1/1) (Htra1 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Magi-1-PDZ-4

Plasmid

#250101

Purpose

Bacterial expression of Magi-1 PDZ domain 4

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Magi-1 (4/6) (Magi1 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Magi-1-PDZ-6

Plasmid

#250102

Purpose

Bacterial expression of Magi-1 PDZ domain 6

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Magi-1 (6/6) (Magi1 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Chapsyn-110-PDZ-2

Plasmid

#250074

Purpose

Bacterial expression of Chapsyn-110 PDZ domain 2

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Chapsyn-110 (2/3) (Dlg2 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Chapsyn-110-PDZ-3

Plasmid

#250075

Purpose

Bacterial expression of Chapsyn-110 PDZ domain 3

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Chapsyn-110 (3/3) (Dlg2 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-Lin7c-PDZ-1

Plasmid

#250096

Purpose

Bacterial expression of Lin7c PDZ domain 1

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

Lin7c (1/1) (Lin7c Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-GRASP55-PDZ-1

Plasmid

#250087

Purpose

Bacterial expression of GRASP55 PDZ domain 1

Depositor

Mark Bedford

Article

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

GRASP55 (1/1) (Gorasp2 Mouse)

Expression

Bacterial

Mutation

None

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-H2B-V5-mScarlet3-S2 (JDW 1529)

Plasmid

#242605

Purpose

A CAGGS driven expression vector containing V5 tagged mScarlet3-S2. H2B for nuclear labeling

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-mScarlet3-S2

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGEN-H2B-V5-mScarlet3-H (JDW 1530)

Plasmid

#242604

Purpose

A CAGGS driven expression vector containing V5 tagged mScarlet3-H. H2B for nuclear labeling

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

H2B-mScarlet3-H

Expression

Mammalian

Promoter

CAGGS

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pME-DRE-nls (JDW 31)

Plasmid

#242561

Purpose

Gateway middle entry clone containing Dre recombinase

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

codon optimized DRE-nls

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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