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Showing: 121 - 140 of 9774 results

  1. BII-gR-PtW-eSpCas9

    Plasmid
    #133357
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
    Depositor
    Blair Madison
    Article
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Insert
    eSpCas9
    Use
    Tags
    HA-tagged eSpCas9
    Expression
    Mammalian
    Mutation
    eSpCas9 mutations
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133357-plasmid-map-sequence-id-267549

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  4. pIRESpuro3+mRFP-Utr_CH

    Plasmid
    #64358
    Purpose
    bicistronic expression of Utrophin-mRFP and Puro resistance
    Depositor
    Matthieu Piel
    Insert
    Utrophin-mRFP (UTRN Human)
    Use
    Tags
    IRES-Puro and mRFP
    Expression
    Mammalian
    Mutation
    Q236R; aa1-261 Utrophin present
    Promoter
    CMV
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    64358-plasmid-map-sequence-id-196884

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  6. rFIXX-bp chain B (IRESpuro)

    Plasmid
    #82451
    Purpose
    Chain B of the habu snake venom FIX/FX binding protein with C-terminal biotin-tag. Cloned into pIRESneo3 (Ampicillin) via 5' NheI and 3' BamHI; IRES vector conferring puromycin resistance.
    Depositor
    Robert Peters
    Insert
    rFIXX-bp chain B
    Use
    Tags
    Biotin
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    T7 RNA polymerase promoter
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    82451-plasmid-map-sequence-id-156993

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  7. pCALNL-eGFP-Esp3I

    Plasmid
    #81226
    Purpose
    Cloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sites
    Depositor
    David Liu
    Article
    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw
    Insert
    Esp31-Neo-Esp31 deletion cassette upstream of EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCBa
    Available since
    March 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    81226-plasmid-map-sequence-id-168098

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  8. mCherry-Espin-Like-C-18

    Plasmid
    #62744
    Purpose
    Localization: Actin Bundling , C1 Cloning Vector, Excitation:587, Emission:610
    Depositor
    Michael Davidson
    Insert
    EspinLike
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. Pdg459-eSp(1.1) V3

    Plasmid
    #226965
    Purpose
    CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226965-plasmid-map-sequence-id-450820

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  10. PDG458 eSp(1.1) V3

    Plasmid
    #226964
    Purpose
    CBh-eSpCas9(1.1)-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226964-plasmid-map-sequence-id-450825

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  11. PX459v3-eSpCas9(1.1)

    Plasmid
    #178800
    Purpose
    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178800-plasmid-map-sequence-id-354839

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  12. eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA

    Plasmid
    #191528
    Purpose
    Vector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabain
    Depositor
    Yannick Doyon
    Insert
    SLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191528-plasmid-map-sequence-id-378116

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  13. pBP-P_TALEsp1_PUPsp1-phlF_PPhlF

    Plasmid
    #204080
    Purpose
    Type 0 promoter part
    Depositor
    Mikhail Shapiro
    Insert
    TALEsp1-stabilized promoter driving phlF repressor; PPhlF DAPG-inducible promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    None
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204080-plasmid-map-sequence-id-413895

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  19. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

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Showing: 121 - 140 of 9774 results