We narrowed to 1,499 results for: Streptococcus

1. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

   Plasmid

   #133344

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_cpaA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3

   Plasmid

   #133343

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_gcrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

   Plasmid

   #133345

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_blaA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA2

   Plasmid

   #133341

   Purpose

   for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the template strand in Caulobacter crescentus

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_gcrA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   constitutive

   Available Since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMJ915

   Plasmid

   #69090

   Purpose

   Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

   Depositor

   Jennifer Doudna

   Article

   Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

   Insert

   cas9
   

   Use

   Tags

   MBP, hexa His, and two SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pScI_dCas9-CDA_J23119-sgRNA

   Plasmid

   #108550

   Purpose

   Bacterial Target-AID vector with sgRNA

   Depositor

   Akihiko Kondo

   Article

   Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

   Inserts

   dCas9-PmCDA1
   Streptococcus pyogenes sgRNA
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   D10A and H840A for SpCas9

   Promoter

   J23119 and lambda OR

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKB01_tagRFP

   Plasmid

   #69103

   Purpose

   Expresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   tagRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWaldo-GFPd_PepTSt

   Plasmid

   #58333

   Purpose

   Expresses the POT family transporter from Streptococcus thermophilus in E. coli as a C-terminally tagged GFP his tagged protein

   Depositor

   Simon Newstead

   Article

   Solcan et al EMBO J. 2012 Aug 15;31(16):3411-21. doi:

   Insert

   PepTSt
   

   Use

   Tags

   GFP-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pX330-Flag-wtSpCas9-H840A

   Plasmid

   #80453

   Purpose

   SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   SpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKB01_mOrange2

    Plasmid

    #69102

    Purpose

    Expresses mOrange2 (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mOrange2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTH1-SpSrtA

    Plasmid

    #64987

    Purpose

    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    S. pyogenes sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-81

    Promoter

    T7 promoter

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCri-H6-TEV-GRAB

    Plasmid

    #128498

    Purpose

    Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
    

    Use

    Tags

    His tag (6x)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. SP-Cas9

    Plasmid

    #62731

    Purpose

    Expresses SP-Cas9 protein in bacterial cells

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    SP-CAS9
    

    Use

    CRISPR

    Tags

    His tag and NLS

    Expression

    Bacterial

    Mutation

    Codon optimazed for e.coli

    Promoter

    T7

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSHS207 - Bacterial expression plasmid for WT SpCas9

    Plasmid

    #101199

    Purpose

    Bacterial expression plasmid for WT SpCas9

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPEPY-PF6-lacI

    Plasmid

    #85589

    Purpose

    Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

    Depositor

    Jan-Willem Veening

    Article

    Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

    Insert

    lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PF6

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15b-ACPs (from S. pneumonia)

    Plasmid

    #63687

    Purpose

    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae

    Depositor

    Michael Johnson

    Insert

    acpS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

17. FU_tetO_dCas9-VP192-T2A-EGFP

    Plasmid

    #121173

    Purpose

    Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

    Depositor

    Timo Otonkoski

    Insert

    dCas9-VP192-T2A-EGFP
    

    Use

    CRISPR and Lentiviral; Inducible by doxycycline

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid

    #106964

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. CROPseq-EFS-HypaSpCas9-P2A-puro

    Plasmid

    #106965

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCRISPRv2_PROX1_1+3

    Plasmid

    #121176

    Purpose

    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

    Depositor

    Timo Otonkoski

    Insert

    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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