We narrowed to 23,876 results for: crispr

1. pCRISPR3_BC2

   Plasmid

   #191857

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR3_BC6

   Plasmid

   #191861

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPR3_BC5

   Plasmid

   #191860

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR3_BC4

   Plasmid

   #191859

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR3_BC3

   Plasmid

   #191858

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR3_BC1

   Plasmid

   #191856

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRISPR_HLA_DPB

   Plasmid

   #164989

   Purpose

   Expression of gRNA targeting HLA-DPB locus, including DPB1*01:01:01

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   gRNA against HLA-DPB1*01:01:01
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CRISPR_HLA_DQB

   Plasmid

   #164991

   Purpose

   Expression of gRNA targeting HLA-DQB locus, including DQB1*05:01:01

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   gRNA against HLA-DQB1*05:01:01
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRISPR_HLA_DRB

   Plasmid

   #164992

   Purpose

   Expression of gRNA targeting HLA-DRB locus, including DRB1*01:02:01

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   gRNA against HLA-DRB1*01:02:01
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRISPR_HLA_DQA

    Plasmid

    #164990

    Purpose

    Expression of gRNA targeting HLA-DQA locus, including DQA1*01:01:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DQA1*01:01:01
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CRISPR_HLA_DPA

    Plasmid

    #164988

    Purpose

    Expression of gRNA targeting HLA-DPA locus, including DPA1*02:01:08

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DPA1*02:01:08
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRISPR2_wt

    Plasmid

    #149386

    Purpose

    P. aeruginosa PA14 CRISPR2 locus

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPR2_-6D

    Plasmid

    #149387

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    6bp deleted downstream of the IHFprox site

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR2_-5D

    Plasmid

    #149388

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    5bp deleted downstream of the IHFprox site

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPR2_+5D

    Plasmid

    #149390

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    5bp inserted downstream of the IHFprox site

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPR2_IHF_Opt

    Plasmid

    #149395

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    IHFprox site is optimized at key positions needed…

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPR2_IHF_Mut

    Plasmid

    #149396

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHF

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    IHFprox site is mutated at key positions needed f…

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPR2_motif_scram

    Plasmid

    #149397

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    Upstream motif recognized by Cas1-2/3 during spac…

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPR

    Plasmid

    #186276

    Purpose

    Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays

    Depositor

    Jennifer Doudna

    Article

    Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

    Insert

    Thiomicrospira shortened CRISPR array
    

    Expression

    Bacterial

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCRISPRv1_COX4

    Plasmid

    #177983

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting COX4

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting COX4
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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