We narrowed to 7,790 results for: nls

1. pCMVMagFRET-1-NLS

   Plasmid

   #50748

   Purpose

   Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

   Depositor

   Maarten Merkx

   Article

   Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

   Insert

   gene encoding for MagFRET-1 with NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR'CMVGFP_NLS_hRIF1(1-1308)

   Plasmid

   #23134

   Depositor

   Lifeng Xu

   Article

   Xu et al J Cell Biol. 2004 Dec 6. 167(5):819-30.

   Insert

   RIF1 deletion construct (RIF1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

   Plasmid

   #79372

   Purpose

   Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-p65-hsf1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWPT-nlsLacZ

   Plasmid

   #12261

   Depositor

   Didier Trono

   Insert

   LacZ
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1xNLS-pMJ915v2

   Plasmid

   #88915

   Purpose

   For expression of modified SpyCas9 in e.coli.

   Depositor

   Jennifer Doudna

   Article

   Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMVMagFRET-6-NLS

   Plasmid

   #50757

   Purpose

   Expresses MagFRET-6 in the nucleus of mammalian cells. Weak affinity control FRET sensor for MagFRET-1

   Depositor

   Maarten Merkx

   Article

   Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

   Insert

   gene encoding for MagFRET-6 with NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3E-2a-6MTnlstdTomato

   Plasmid

   #67699

   Purpose

   3' entry clone with 6xmyc nuclear localized tdTomato

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   tdTomato
   

   Use

   Tags

   2A-6xMyc-NLS

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. R26/P-nlsrtTA

   Plasmid

   #46183

   Depositor

   Rudolf Jaenisch

   Article

   Wutz et al Nat Genet. 2002 Feb;30(2):167-74. Epub 2

   Inserts

   nlsrtTA
   pgk-puro
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 19, 2013

   Availability

   Academic Institutions and Nonprofits only

9. NLS-GAI(1-92)

   Plasmid

   #37317

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

   Insert

   GAI (GAI Mustard Weed)
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   contains aa 1-92

   Promoter

   CMV

   Available since

   June 15, 2012

   Availability

   Academic Institutions and Nonprofits only

10. p3E-CMVmin:nlsGFP pA

    Plasmid

    #80821

    Purpose

    CMVmin-promoted nlsGFP with pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    p3E-CMV-nlsGFP pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVmin

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNLS-LSSmKate1-N1

    Plasmid

    #31870

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

    Promoter

    CMV

    Available since

    Sept. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+ oDam_f_GFPoNLS

    Plasmid

    #85819

    Purpose

    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    oDam_f_GFPoNLS
    

    Use

    Tags

    oNLS (optimized nuclear localization signal) C te…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNLS-LSSmKate2-N1

    Plasmid

    #31871

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Promoter

    Available since

    Sept. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pME-6MTnlstdTomato2A

    Plasmid

    #67685

    Purpose

    Middle entry nuclear localized tdTomato reporter with 6xMT and 2A

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    tdTomato
    

    Use

    Tags

    2A and 6xMyc-NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. LI B-C N-NLS KOZ c2

    Plasmid

    #54443

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Insert

    Nuclear localization signal (NLS) of SV40 large T antigen
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3E-nlsGFP pA

    Plasmid

    #80814

    Purpose

    nlsGFP with pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    nlsGFPpA 3'E
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCNL-N1_3xNLS

    Plasmid

    #65673

    Purpose

    Expresses NLS-CNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    three copies of SV40 nuclear localization signal
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYNL-N1_3xNLS

    Plasmid

    #65672

    Purpose

    Expresses NLS-YNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    three copies of SV40 nuclear localization signal
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pONL-N1_3xNLS

    Plasmid

    #65674

    Purpose

    Expresses NLS-ONL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    three copies of SV40 nuclear localization signal
    

    Use

    Luciferase

    Tags

    ONL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA (AAV6)

    Viral Prep

    #61592-AAV6

    Purpose

    Ready-to-use AAV6 particles produced from pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA (#61592). In addition to the viral particles, you will also receive purified pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA plasmid DNA. CMV-driven expression of SaCas9. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Promoter

    Tags

    None

    Available since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only