We narrowed to 6,508 results for: nls
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Plasmid#190034Purposevector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promotersDepositorInserthumanized hfCas13d
ExpressionMammalianMutationA134V,A140V,A141V,A143VPromoterCBh, SV40, hU6Available SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 (PX165)
Plasmid#48137PurposeHuman codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)DepositorInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#190033Purposevector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promotersDepositorInserthumanized hfCas13X
ExpressionMammalianMutationY672A,Y676APromoterCBh, SV40, hU6Available SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
HyPer7.2DAAO-NLS
Plasmid#168302PurposeChemogenetic fluorescent H2O2 reporter targeted to the nucleusDepositorInsertHyPer7.2DAAO-NLS
ExpressionMammalianPromoterCMVAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
epB_UbC_CymRV5-nls-GFP_DEx2
Plasmid#119906PurposeePiggyBac mammalian expression plasmid for CymR-GFP fusionDepositorInsertCymRV5-nls-GFP
ExpressionMammalianAvailable SinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
3xHA-miniTurbo-NLS_pCDNA3
Plasmid#107172Purposeexpresses 3xHA-tagged miniTurbo in the mammalian nucleusDepositorInsertminiTurbo (BirA mutant)
ExpressionMammalianMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHEE401
Plasmid#71286PurposeEgg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistanceDepositorInsertssgRNA scaffold
zCas9
UseCRISPR; Plant binary vectorTags3x FLAG and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterEC1.2 promoter and U6-26p Arabidopsis U6 gene pro…Available SinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1658-dCas9-EGFP
Plasmid#51023PurposeTemplate for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)DepositorInsertdCas9 fuse to EGFP
UseCRISPR and RetroviralTagsEGFPExpressionMammalianPromoterMSCV LTR promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 Utr230-EGFP-3XNLS
Plasmid#58466PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the first 230 amino acids of human utrophin, on a CMV promoterDepositorInsertsTagsEGFPExpressionMammalianPromoterCMVAvailable SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHM840
Plasmid#20701DepositorInsertGFP-NLS-lacZ
ExpressionMammalianAvailable SinceApril 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pK036.TRE-Flpe-WPRE (Supernova)
Plasmid#85007PurposeFor Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.DepositorInsertNLS-FLPe-WPRE
ExpressionMammalianAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS
Plasmid#60904PurposeThe plasmid encodes a antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a transcriptional activation domain VP64DepositorInsertscFv-GCN4
UseLentiviralExpressionMammalianAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
BPK2139
Plasmid#65776PurposeHuman expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAGDepositorInsertmammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
UseCRISPRTagsNLS-3xFLAGExpressionMammalianPromoterCAGAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS-HA-Dre
Plasmid#51272PurposeComponent of the Co-Driver binary site-specific recombinase system, expression of the site-specific recombinase Dre (mouse codon-optimized)DepositorInsertDre
TagsNLS-HAExpressionMammalianPromoterCAGAvailable SinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-NLS
Plasmid#39319DepositorInsertmCherry-3xNLS
Tags3xSV40LT NLSExpressionMammalianPromoterCMVAvailable SinceNov. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
p6XHis_NLS-SaCas9
Plasmid#101086PurposeExpress SaCas9 in bacteria with a 6xHis tag for purificationDepositorInsertCRISPR-associated protein Cas9/Csn1
UseCRISPRTagsTrxA-6xHis-STagExpressionBacterialPromoterT7lacAvailable SinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHSE401
Plasmid#62201PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoter35S and AtU6-26pAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-NLS-R-GECO
Plasmid#32462PurposeMammalian expression of nucleus-targeting red fluorescent genetically encoded Ca2+ indicator for optical imagingDepositorInsertR-GECO1.0
TagsNLS sequence DPKKKRKVExpressionMammalianMutationSubstitutions relative to the mApple-derived anal…PromoterCMVAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLVX-XBP1 mNeonGreen NLS
Plasmid#115968PurposeER-stress sensor - XBP1 splicing fluorescent reporter with mNeonGreenDepositorInsertX-box binding protein 1 (XBP1 Synthetic, Human)
UseLentiviralTagsHA tag, c-myc NLS, and mNeonGreenExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP
Plasmid#86827PurposeExpresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAsDepositorInsertFLAG-NLS-MS2-eGFP
ExpressionMammalianPromoterCMVAvailable SinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only