We narrowed to 5,806 results for: pcas

1. pCAGGS_VP30_MARV

   Plasmid

   #135472

   Purpose

   Mammalian expression vector encoding Marburg virus VP30 gene under control of the AG promoter

   Depositor

   Elke Mühlberger

   Article

   Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

   Insert

   VP30
   

   Expression

   Mammalian

   Mutation

   None

   Promoter

   AG

   Available Since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. DS-SPcas

   Plasmid

   #48645

   Purpose

   Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   tracrRNA precursor
   

   Use

   CRISPR

   Promoter

   proC and tracrRNA promoter

   Available Since

   Dec. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCasTet-λ

   Plasmid

   #128318

   Purpose

   Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

   Depositor

   Christopher Johnston

   Article

   Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

   Insert

   TetR and pTetR/TetO
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Mutation

   Constitutive expression of cas9 and anhydro-tetra…

   Promoter

   tet promoter

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAT003

   Plasmid

   #171628

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mNeon
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CAG and EF1-a

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9_human_ADAR2_ccB

   Plasmid

   #158113

   Purpose

   deletion hADAR2

   Depositor

   Emanuela Felley-Bosco

   Article

   Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

   Insert

   ADAR2 (ADARB1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9_human_ADAR2_ccA

   Plasmid

   #158114

   Purpose

   deletion hADAR2

   Depositor

   Emanuela Felley-Bosco

   Article

   Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

   Insert

   ADAR2 (ADARB1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAT100

   Plasmid

   #180512

   Purpose

   Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   PlmCasX with DpbCasX R1 loop
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA_e-1

   Plasmid

   #202096

   Purpose

   Receiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pCA_o-1

   Plasmid

   #202091

   Purpose

   Receiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Mutation

   None

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCAP01

    Plasmid

    #59981

    Purpose

    The function of this material is to facilitate the capture and expression of bacterial gene clusters.

    Depositor

    Bradley Moore

    Article

    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

    Type

    Empty backbone

    Use

    Synthetic Biology; Transformation-associated reco…

    Tags

    no

    Expression

    Bacterial and Yeast

    Promoter

    no

    Available Since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGMKOSiE

    Plasmid

    #20865

    Depositor

    Keisuke Kaji

    Article

    Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

    Insert

    myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)
    

    Expression

    Mammalian

    Available Since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAS9c

    Plasmid

    #141250

    Purpose

    Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAT219

    Plasmid

    #134887

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAROTENE5X

    Plasmid

    #208816

    Purpose

    Encodes bacterial crtEBIY operon under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cell

    Depositor

    Marc Erhardt

    Article

    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

    Insert

    Five copies of JUB1 binding site within the synthetic promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    JUB1 TF-responsive promoter

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAT527

    Plasmid

    #180514

    Purpose

    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAS9id

    Plasmid

    #141251

    Purpose

    Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9

    Plasmid

    #162606

    Purpose

    Pre-express Cas9 in yeast

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9
    

    Expression

    Yeast

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGTetRnls

    Plasmid

    #26599

    Depositor

    Peter Andrews

    Article

    Zafarana et al Stem Cells. 2009 Apr . 27(4):776-82.

    Insert

    Tetracycline repressor (TetR E.Coli)
    

    Tags

    nls

    Expression

    Mammalian

    Available Since

    Nov. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAS9cd

    Plasmid

    #141252

    Purpose

    Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCas9_sgRNA_0

    Plasmid

    #70763

    Purpose

    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating

    Depositor

    Regine Kahmann

    Article

    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10

    Inserts

    U6 promoter
    cas9
    tnos terminator
    

    Use

    Self-replicating in ustilago maydis, conferring c…

    Tags

    N-terminal NLS, C-terminal HA-tag +NLS

    Mutation

    Streptococcus pyogenes gene codon-optimized for U…

    Promoter

    U6 from Ustilago maydis and synthetic otef promot…

    Available Since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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