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Showing: 2801 - 2840 of 8751 results

  12. pJT1

    Plasmid
    #98299
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.
    Insert
    PTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    98299-plasmid-map-sequence-id-189966

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  14. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    113631-plasmid-map-sequence-id-222216

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  15. pJAID5XY

    Plasmid
    #165051
    Purpose
    Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
    Depositor
    Claudia Vickers
    Article
    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    OsTIR1 is codon-optimised for expression in S. ce…
    Promoter
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    165051-plasmid-map-sequence-id-327149

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  18. JPF0510

    Plasmid
    #124001
    Purpose
    Encodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector
    Depositor
    Hana El-Samad
    Article
    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2
    Insert
    PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124001-plasmid-map-sequence-id-252358

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  21. pJAID5VY

    Plasmid
    #165048
    Purpose
    Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
    Depositor
    Claudia Vickers
    Article
    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    OsTIR1 is codon-optimised for expression in S. ce…
    Promoter
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    165048-plasmid-map-sequence-id-327148

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  28. ipYUC2Cas-LT1

    Plasmid
    #210559
    Purpose
    Binary vector expressing the estradiol-inducible Lineage Tracing system. XVE expression driven by M. polymorpha YUC2 promoter.
    Depositor
    Ortrun Mittelsten Scheid
    Article
    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16
    Insert
    pMpEF1::XVE::UBQ10t::LexA::SpCas9::PEA3AT::pU6::AAVS1gRNA::pMpEF1::LT1::Hygromycin
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    210559-plasmid-map-sequence-id-426227

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  29. L1-AA027

    Plasmid
    #185752
    Purpose
    Level 1 loop assembly vector containing a transcriptional unit with a Hygromycin resistance cassette under the control of the A. agrestis EF1a promoter (pAaEF1a).
    Depositor
    Peter Szoevenyi
    Article
    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph
    Insert
    HygR2
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    185752-plasmid-map-sequence-id-367857

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  30. pJAID5WY

    Plasmid
    #165050
    Purpose
    Integrative expression of SOD1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
    Depositor
    Claudia Vickers
    Article
    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>SOD1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    OsTIR1 is codon-optimised for expression in S. ce…
    Promoter
    Available since
    March 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    165050-plasmid-map-sequence-id-327682

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  31. VanR - F165V

    Plasmid
    #158965
    Purpose
    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.
    Depositor
    Michael Jensen
    Article
    et al Biotechnology Notes, Volume 1, 2020, Pag
    Insert
    VanR - F165V
    Use
    Tags
    Expression
    Yeast
    Mutation
    Wild type VanR from Caulobacter crescentus with m…
    Promoter
    TEF1
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    158965-plasmid-map-sequence-id-319279

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  32. VanR - F165R

    Plasmid
    #158966
    Purpose
    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.
    Depositor
    Michael Jensen
    Article
    et al Biotechnology Notes, Volume 1, 2020, Pag
    Insert
    VanR - F165R
    Use
    Tags
    Expression
    Yeast
    Mutation
    Wild type VanR from Caulobacter crescentus with m…
    Promoter
    TEF1
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    158966-plasmid-map-sequence-id-319292

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  39. SZ_EC_02

    Plasmid
    #155341
    Purpose
    Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR
    Depositor
    Jens Nielsen
    Article
    Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:
    Inserts
    tef1 promoter - xylulose kinase - pex20 terminator
    pyk1 promoter - xylose reductase - pex16 terminator
    gapdh promoter - xylitol dehydrogenase - lip2 terminator
    Use
    Easycloneyali insert vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    gapdh, pyk1, and tef1
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    155341-plasmid-map-sequence-id-316921

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  40. pCfB2793

    Plasmid
    #64043
    Purpose
    pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.
    Depositor
    Irina Borodina, Jerome Maury
    Article
    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi
    Insert
    GFP
    Use
    Cre/Lox
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1 promoter and none
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    64043-plasmid-map-sequence-id-149101

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Showing: 2801 - 2840 of 8751 results