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pQCXIP HBT-Ubiquitin

Plasmid

#26865

Depositor

Peter Kaiser

Publication

Meierhofer et al J Proteome Res. 2008 Oct . 7(10):4566-76

Insert

ubiquitin

Use

Retroviral

Tags

HBT

Expression

Mammalian

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDNA-mSin1(1.1)-HA

Plasmid

#73388

Purpose

Expresses mSin-HA

Depositor

Jie Chen, Taekjip Ha

Publication

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

mSin1 transcript variant protein isoform 1 (MAPKAP1 Human)

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJ3 SHP2 D61A

Plasmid

#8320

Depositor

Ben Neel

Publication

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Mutation

D61A (activated mutant)

Available since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
pMON-HsNMT1

Plasmid

#67475

Purpose

Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

Depositor

Richard Kahn

Publication

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

HsNMT1 (NMT1 Human)

Expression

Bacterial

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMito-PAGFP-FRB.

Plasmid

#60005

Purpose

Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

Depositor

Stephen Royle

Publication

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Type

Empty backbone

Use

Knocksideways

Tags

FRB and PAGFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGT-B1

Plasmid

#122579

Purpose

MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Publication

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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(R42A) Cyclin B1-GFP (62)

Plasmid

#61849

Purpose

mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

Depositor

Jonathon Pines

Publication

Clute et al Nat Cell Biol. 1999 Jun;1(2):82-7.

Insert

cyclin B1 (CCNB1 Human)

Tags

GFP and Myc

Expression

Mammalian

Mutation

V5A R42A

Promoter

CMV

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET28a-Ec.coaE (pESC124)

Plasmid

#50390

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

Depositor

Tadhg Begley, Erick Strauss

Publication

Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

Insert

DPCK

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HA-GABARAPL1

Plasmid

#137759

Purpose

Expression of GABARAPL1

Depositor

Carol Mercer

Publication

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAPL1 (GABARAPL1 Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-6xHis

Plasmid

#87343

Purpose

To express EGFP-6xHis in mammalian cell

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

6xHis

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-mZscan4d-polyA

Plasmid

#61831

Purpose

For mRNA synthesis or expression in mammalian cells

Depositor

Yi Zhang

Publication

Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

Insert

Zscan4d (Zscan4d Mouse)

Tags

flag

Expression

Mammalian

Promoter

CMV and T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3B-BE3

Plasmid

#113411

Purpose

Expresses hA3B-BE3 in mammalian cells

Depositor

Jia Chen

Publication

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3B-BE3 (APOBEC3B S. pyogenes and Bacteriophage PBS2, Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Oct. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ER-RpHLuorin2

Plasmid

#171717

Purpose

Luminal ER expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Publication

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 targeted to ER

Tags

KDEL ER-retention signal and Signal sequence of h…

Expression

Mammalian

Promoter

CMV

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-DEPTOR

Plasmid

#73390

Purpose

Expresses HA and YFP tagged DEPTOR

Depositor

Jie Chen, Taekjip Ha

Publication

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

DEP Domain Containing MTOR-Interacting Protein (DEPTOR Human)

Tags

HA and YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pJRT.Agro.CLCrVA.008

Plasmid

#31809

Depositor

Niki Robertson

Publication

Tuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi:

Insert

JRTCLCrVA.008

Use

Agrobacterium binary

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Col1A-5'probe

Plasmid

#20732

Depositor

Rudolf Jaenisch

Publication

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

Col1A-5'probe (Col1a1 Mouse)

Expression

Bacterial

Available since

April 24, 2009

Availability

Academic Institutions and Nonprofits only

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Lenti-dCas9-TurboID-blast

Plasmid

#222599

Purpose

Express fussed dCas9-TurboID for proximity labeling

Depositor

Yarui Diao

Publication

Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

Insert

dCas9-TurboID

Use

Lentiviral

Promoter

EF-1α

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET30-RT1306

Plasmid

#131521

Purpose

Expresses engineered RT-1306 with His-tag

Depositor

Bryan Dickinson

Publication

Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

Insert

RT-1306

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCoofy11

Plasmid

#44002

Depositor

Sabine Suppmann

Publication

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10

Expression

Insect

Promoter

hr5

Available since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pScalps_Puro_mTet2 catalytic domain HxD

Plasmid

#79611

Purpose

Expression of catalytically inactive mouse Tet2

Depositor

Silvia Monticelli

Publication

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

Mutant Tet2 with H1302Y, D1304A substitutions in …

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles