Addgene

We narrowed to 23,543 results for: CHI

Showing: 3651 - 3700 of 23543 results

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pMAL-c5X-Darpin E_01

Plasmid

#66996

Purpose

Expresses malE-Darpin E_01 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-Darpin E_01

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-poly-RGD

Plasmid

#67002

Purpose

Expresses malE-poly-RGD in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-poly-RGD

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCRPVsheLL

Plasmid

#46952

Purpose

encode CRPV L1 and L2, Bicistronic, too large to self-package

Depositor

John Schiller

Article

Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 200

Insert

CRPV L1+L2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pJS61

Plasmid

#207585

Purpose

GFP-TolA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TolA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pSVD5

Plasmid

#18877

Depositor

Jay Keasling

Article

Van Dien et al Appl Environ Microbiol. 1997 May . 63(5)

Insert

exopolyphosphatase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only
BiDi-[mp-mCherry2]mp-mEGFP

Plasmid

#182871

Purpose

Bi-directional expression vector of monomers with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al Biophys J. 2021 Dec 21;120(24):5478-5490

Insert

myr-palm-mCherry2, myr-palm-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCZGY2162

Plasmid

#110136

Purpose

Epidermal Mitochondrial-tagged Florescent Calcium Sensor

Depositor

Andrew Chisholm

Article

Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

Insert

mito::GCaMP3

Use

Tags

mito::GCaMP3

Expression

Worm

Mutation

Promoter

Pcol-19

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCZGY2840

Plasmid

#87764

Purpose

Pmec-4-PH::miniSOG, expression of membrane targeted PH-miniSOG in mechanosensory neurons

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmec-4-PH-miniSOG

Use

Tags

PH

Expression

Worm

Mutation

Promoter

Pmec-4

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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EcAvs2

Plasmid

#188789

Purpose

Avs2 from Escherichia coli NCTC9087

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
myr-palm-mNeonGreen(2x)

Plasmid

#197919

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mNeonGreen-NeonGreen

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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myr-palm-mNeonGreen

Plasmid

#197918

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26-CNTnw

Plasmid

#100163

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Use

Tags

MBP-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-ELP[V5A2G3-60]

Plasmid

#67009

Purpose

Expresses malE-ELP[V5A2G3-60] in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-ELP[V5A2G3-60]

Use

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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dg264_pENTR_ChronosCel

Plasmid

#66102

Purpose

Entry clone containing Chronos coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

Depositor

Dominique Glauser

Article

Schild et al Genetics. 2015 May 28. pii: genetics.115

Insert

Chronos

Use

Gateway

Tags

Expression

Mutation

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBT225_(pCA-tdT3Myc)

Plasmid

#36873

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tdTomato-3Myc

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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YFP-Gamma 9-3

Plasmid

#36074

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

G protein G9(gamma 3 tail)

Use

Tags

YFP

Expression

Mammalian

Mutation

HIs tag between YFP and insert

Promoter

CMV

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
SF-1 shRNA #1

Plasmid

#28186

Depositor

Bernard Schimmer

Article

Rui et al Endocrinology. 2008 Jul . 149(7):3668-78

Insert

Nr5a1 shRNA (Nr5a1 Mouse)

Use

RNAi

Tags

cGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pSPK1

Plasmid

#18876

Depositor

Jay Keasling

Article

Van Dien et al Appl Environ Microbiol. 1997 May . 63(5)

Insert

polyphosphate kinase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only
CDC12(K351N)

Plasmid

#1163

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Use

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

K351N

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG aconitase GFP

Plasmid

#66104

Purpose

expression of chicken aconitase GFP

Depositor

Martin Scaal, Felicitas Proels

Article

et al Development. 2015 Feb 15;142(4):665-71.

Insert

aconitase

Use

Chicken expression

Tags

EGFP

Expression

Mutation

Promoter

CAG

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only
GAL-CDC12(K351N)

Plasmid

#1166

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

K351N

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
CDC12(E368Q)

Plasmid

#1167

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Use

Tags

266bp of upstream sequence

Expression

Yeast

Mutation

E368Q

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pGALSc104(G217S/T499I)

Plasmid

#1151

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

G217S/T499I

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pET26-CNTN149L

Plasmid

#100164

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Use

Tags

MBP-His

Expression

Bacterial

Mutation

N149L

Promoter

T7

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL-CDC12(E368Q)

Plasmid

#1164

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

E368Q

Promoter

GAL

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pchfn01

Plasmid

#14061

Depositor

Richard Hynes

Article

Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

Insert

Chicken Fibronectin (FN1 Chicken)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
TM104b(A503V/A509D)

Plasmid

#1161

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

A503V/A509D

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
SF-1 shRNA #2

Plasmid

#28187

Depositor

Bernard Schimmer

Article

Rui et al Endocrinology. 2008 Jul . 149(7):3668-78

Insert

Nr5a1 shRNA (Nr5a1 Mouse)

Use

RNAi

Tags

cGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
SF-1 shRNA #3

Plasmid

#28188

Depositor

Bernard Schimmer

Article

Rui et al Endocrinology. 2008 Jul . 149(7):3668-78

Insert

Nr5a1 shRNA (Nr5a1 Mouse)

Use

RNAi

Tags

cGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
TM104b(G217S/T499I)

Plasmid

#1160

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

HSP104 (HSP104 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

G217S/T499I

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pT-36

Plasmid

#16252

Depositor

Yoshiko Kikuchi

Article

Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

Insert

CDC3 (CDC3 Budding Yeast)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pTYB1-T4gp46

Plasmid

#228211

Purpose

Expression in BL21(DE3) of T4 SbcC-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

46

Use

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SJZ6-HIS5

Plasmid

#228259

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

his5

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1 S.c.

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-BmTyr-H2B

Plasmid

#222941

Purpose

H2B-BmTyr fusion

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

BmTyr-H2B

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDWJ16

Plasmid

#207599

Purpose

GFP-BAA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BAA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDWJ26

Plasmid

#207603

Purpose

GFP-AIA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AIA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDWJ22

Plasmid

#207602

Purpose

GFP-AABA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AABA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDWJ18

Plasmid

#207601

Purpose

GFP-ABA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-ABA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDWJ17

Plasmid

#207600

Purpose

GFP-ABB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-ABB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDWJ15

Plasmid

#207598

Purpose

GFP-BBA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BBA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDWJ14

Plasmid

#207597

Purpose

GFP-AABB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AABB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDWJ13

Plasmid

#207596

Purpose

GFP-AAB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMG2

Plasmid

#207595

Purpose

GFP-BAB expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-BAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJS109

Plasmid

#207590

Purpose

GFP-ABBA expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-ABBA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
myr-palm-Gamillus(2x)

Plasmid

#197921

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus-Gamillus

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GPI-mGreenLantern(2x)

Plasmid

#197917

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mGreenLantern-mGreenLantern

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GPI-Gamillus(2x)

Plasmid

#197915

Purpose

Homo-dimer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus-Gamillus

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
myr-palm-Gamillus

Plasmid

#197920

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EcAvs1

Plasmid

#188787

Purpose

Avs1 from Escherichia coli NCTC9036

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pTorD-H6

Plasmid

#169039

Purpose

Escherichia coli TorD with C-terminal 6xHis in pET28a.

Depositor

Siegfried Musser

Article

Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

Insert

TorD-H6

Use

Tags

6xHis

Expression

Bacterial

Mutation

The mutation N at position 46 was changed back to…

Promoter

T7

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 3651 - 3700 of 23543 results

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