We narrowed to 29,263 results for: URE

1. pFastBac dual_Human full length _CDK7 (S164D, T170E)_del327-346

   Plasmid

   #217012

   Purpose

   Expression of CDK7 in insect cells

   Depositor

   Manjeet Mukherjee

   Article

   Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

   Insert

   CDK7 (S164D, T170E)_del327-346 (CDK7 Human)
   

   Use

   Tags

   TEV cleavable his tag

   Expression

   Insect

   Mutation

   (S164D, T170E)_del327-346

   Promoter

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFastBac dual_Human full length CDK7(W132R, S164D, T170E)

   Plasmid

   #217013

   Purpose

   Expression of CDK7 in insect cells

   Depositor

   Manjeet Mukherjee

   Article

   Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

   Insert

   CDK7(W132R, S164D, T170E) (CDK7 Human)
   

   Use

   Tags

   TEV cleavable his tag

   Expression

   Insect

   Mutation

   CDK7(W132R, S164D, T170E)

   Promoter

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized

   Plasmid

   #184563

   Purpose

   CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

   Depositor

   Kacper Rogala

   Article

   Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

   Insert

   C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   All lysines were mutated to arginines. Codon-opti…

   Promoter

   CMV

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. xCas9 3.7

   Plasmid

   #108379

   Purpose

   Mammalian expression vector for xCas9 3.7

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9 3.7
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Pediculus humanus corporis PINK1 (115 - 575)

   Plasmid

   #110750

   Purpose

   Bacterial expression of PhPINK1 (biochemistry)

   Depositor

   David Komander

   Article

   Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

   Insert

   Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
   

   Use

   Tags

   N-His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. xCas9 3.6

   Plasmid

   #108384

   Purpose

   Mammalian expression vector for xCas9 3.6

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9 3.6
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Promoter

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTH1-SaSrtA

   Plasmid

   #64973

   Purpose

   Expresses the wild type of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   T7 promoter

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MSP2283

   Plasmid

   #70702

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MSP2266

   Plasmid

   #70705

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCX4-EGFP

    Plasmid

    #36120

    Depositor

    Larry Goldstein

    Article

    Israel et al Nature. 2012 Jan 25;482(7384):216-20. do

    Insert

    eGFP (eGFP )
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. Pediculus humanus corporis PINK1 (143 - 575)

    Plasmid

    #110751

    Purpose

    Bacterial expression of PhPINK1 (crystallography)

    Depositor

    David Komander

    Article

    Schubert et al Nature. 2017 Dec 7;552(7683):51-56. doi:

    Insert

    Phum_PHUM577390 (Phum_PHUM577390 Pediculus humanus corporis)
    

    Use

    Tags

    N-His6-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMB38-HF380

    Plasmid

    #37332

    Depositor

    Takahide Kon

    Article

    Kon et al Nature. 2012 Mar 7;484(7394):345-50. doi

    Insert

    Cytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)
    

    Use

    Dictyostelium expression

    Tags

    His6 and FLAG tag

    Expression

    Mutation

    deleted amino acids 1-1387

    Promoter

    TRE-Pmin

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

13. tdEos-Vinculin-14

    Plasmid

    #57691

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pTH1-SaSrtA-KA

    Plasmid

    #64981

    Purpose

    Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108A

    Promoter

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mEos2-Talin-ABM-10

    Plasmid

    #57421

    Purpose

    Localization: Talin Actin Binding Module, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Talin AMB
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Actin Binding Module; I/LWEQ = 197 amino acids fr…

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pTH1-SaSrtA-KQ

    Plasmid

    #64982

    Purpose

    Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, E105K, E108Q

    Promoter

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMB38-HF380deltaMTBD

    Plasmid

    #37333

    Depositor

    Takahide Kon

    Article

    Kon et al Nature. 2012 Mar 7;484(7394):345-50. doi

    Insert

    Cytoplasmic Dynein Heavy Chain (dhcA Dictyostelium discoideum)
    

    Use

    Dictyostelium expression

    Tags

    His6 and FLAG tag

    Expression

    Mutation

    deleted amino acids 1-1387 and replaced amino aci…

    Promoter

    TRE-Pmin

    Available Since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

18. MT363

    Plasmid

    #65858

    Purpose

    The gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.

    Depositor

    Detlef Weigel

    Article

    Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

    Insert

    nahG
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. xCas9(3.7)-BE4

    Plasmid

    #108381

    Purpose

    Mammalian expression vector for xCas9(3.7)-BE4

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.7)-BE4
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. xCas9(3.7)-BE3

    Plasmid

    #108380

    Purpose

    Mammalian expression vector for xCas9(3.7)-BE3

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.7)-BE3
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge