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lenti-sgRNA hygro

Plasmid

#104991

Purpose

This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA hygro

Plasmid

#104991

Purpose

This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Flag-His-ATM wt

Plasmid

#31985

Purpose

Mammalian expression of human ATM with Flag and His tags

Depositor

Michael Kastan

Article

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM wild-type (ATM Human)

Use

Tags

Flag and HIS

Expression

Mammalian

Mutation

3' UTR of 400 nucl.

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Flag-His-ATM wt

Plasmid

#31985

Purpose

Mammalian expression of human ATM with Flag and His tags

Depositor

Michael Kastan

Article

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM wild-type (ATM Human)

Use

Tags

Flag and HIS

Expression

Mammalian

Mutation

3' UTR of 400 nucl.

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pET-Cas9-NLS-6xHis

Plasmid

#62933

Purpose

Expression of Cas9-NLS-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cas9-NLS-6xHis

Use

Tags

6xHis tag and NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pET-Cas9-NLS-6xHis

Plasmid

#62933

Purpose

Expression of Cas9-NLS-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cas9-NLS-6xHis

Use

Tags

6xHis tag and NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCas

Plasmid

#62225

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgR

Depositor

Sheng Yang

Article

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Has Service

Cloning Grade DNA

Insert

cas9

Use

CRISPR; Lambda red recombinase

Tags

Expression

Bacterial

Mutation

Promoter

native cas9 promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCas

Plasmid

#62225

Purpose

Constitutive expression of cas9 and inducible expression of lambda RED and sgR

Depositor

Sheng Yang

Article

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Has Service

Cloning Grade DNA

Insert

cas9

Use

CRISPR; Lambda red recombinase

Tags

Expression

Bacterial

Mutation

Promoter

native cas9 promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-Talin head-SpyCatcher003

Plasmid

#133566

Purpose

Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

EGFP-Talin head-SpyCatcher003

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Talin head-SpyCatcher003

Plasmid

#133566

Purpose

Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

EGFP-Talin head-SpyCatcher003

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Luke-2

Plasmid

#228900

Purpose

Luke-2 localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFP

Depositor

John Samuelson

Article

Kanakapura Sundararaj et al mSphere. 2024 Sep 25;9(9):e0046624. doi:

Insert

Carbohydrate binding domain cbm49 protein

Use

Acanthamoeba expression vector

Tags

eGFP

Expression

Mutation

none

Promoter

Own

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CD14

Plasmid

#13645

Depositor

Doug Golenbock

Insert

CD14 (CD14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CD14

Plasmid

#13645

Depositor

Doug Golenbock

Insert

CD14 (CD14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pDG1-PHP.eB

Plasmid

#220799

Purpose

AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

E4, E2A, VA, AAV2 rep and PHP.eB cap genes

Use

AAV; Aav helper vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-puro-ARID1A

Plasmid

#39478

Purpose

Tetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vector

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

ARID1A (ARID1A Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-puro-ARID1A

Plasmid

#39478

Purpose

Tetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vector

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

ARID1A (ARID1A Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pM2s2AsG

Plasmid

#137924

Purpose

E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Insert

Superfolder GFP

Use

Synthetic Biology; For use with e. coli nissle in…

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pM2s2AsG

Plasmid

#137924

Purpose

E. coli Nissle pMUT2-derived plasmid with ampicillin resistance and constitutive GFP expression

Depositor

Neel Joshi

Article

Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

Insert

Superfolder GFP

Use

Synthetic Biology; For use with e. coli nissle in…

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP Neo (657-2)

Plasmid

#17447

Purpose

3rd gen lentiviral eGFP expression vector, CMV promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP Neo (657-2)

Plasmid

#17447

Purpose

3rd gen lentiviral eGFP expression vector, CMV promoter, Neo

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pUB_smHA_KDM5B_MS2

Plasmid

#81085

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smHA_KDM5B_24xMS2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pUB_smHA_KDM5B_MS2

Plasmid

#81085

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smHA_KDM5B_24xMS2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pUC57-gCarvi-T2A

Plasmid

#190333

Purpose

Fluorescent reporter for cAMP

Depositor

Naoto Saitoh

Article

Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Insert

gCarvi

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-OsTIR1(F74G)

Plasmid

#140730

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-OsTIR1(F74G)

Plasmid

#140730

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G)-P2A-mAID-EGFP-NES

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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PX458-AAVS1

Plasmid

#113194

Purpose

Encodes gRNA for human AAVS1

Depositor

Adam Karpf

Insert

AAVS1 (PPP1R12C, AAVS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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PX458-AAVS1

Plasmid

#113194

Purpose

Encodes gRNA for human AAVS1

Depositor

Adam Karpf

Insert

AAVS1 (PPP1R12C, AAVS1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Golgi-7

Plasmid

#55052

Purpose

Localization: Golgi Complex, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Golgi (B4GALT1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-Golgi-7

Plasmid

#55052

Purpose

Localization: Golgi Complex, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Golgi (B4GALT1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO mCherry (AAV2)

Viral Prep

#114471-AAV2

Purpose

Ready-to-use AAV2 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Promoter

EF1a

Tags

mCherry (Flp-dependent)

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only