We narrowed to 58,402 results for: cloning

1. XClone-Cas9-Cronus-T2A-BFP

   Plasmid

   #141209

   Purpose

   Tunable and temporal expression control of Cas9

   Depositor

   Rory Johnson

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. HH13A Clone 13A heavy chain

   Plasmid

   #192178

   Purpose

   Clone 13A heavy chain (HH13A)

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 13A heavy chain (HH13A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. HK13A Clone 13A light chain

   Plasmid

   #192182

   Purpose

   Clone 13A light chain (HK13A)

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 13A light chain (HK13A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET His6 TEV LIC cloning vector (2B-T)

   Plasmid

   #29666

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET mCherry LIC cloning vector (u-mCherry)

   Plasmid

   #29769

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET His6 LIC cloning vector (2Bc-T)

   Plasmid

   #37236

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET Flag TEV LIC cloning vector (2L-T)

   Plasmid

   #29715

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   FLAG-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET His6 TEV LIC cloning vector (1B)

   Plasmid

   #29653

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector

   Plasmid

   #139015

   Purpose

   To express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targeting

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   HA-MAO

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXR004: CasRx pre-gRNA cloning backbone

    Plasmid

    #109054

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET tdTomato LIC cloning vector (H6-tdTomato)

    Plasmid

    #86928

    Purpose

    pET LIC cloning vector for Biotin-His6-tev-yORF-tdTomato

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and td…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET StrepII TEV LIC cloning vector (1R)

    Plasmid

    #29664

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Strep II and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET GFP LIC cloning vector (u-msfGFP)

    Plasmid

    #29772

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    SiriusGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET mOrange LIC cloning vector (u-mOrange)

    Plasmid

    #29770

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    mOrange

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC003 - LshC2C2 locus into pACYC184 for spacer cloning

    Plasmid

    #79152

    Purpose

    LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    LshC2c2 locus
    

    Use

    Tags

    Expression

    Mutation

    *

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET GFP LIC cloning vector (2GFP-T)

    Plasmid

    #29716

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, SiriusGFP, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac His6 TEV LIC cloning vector (4B)

    Plasmid

    #30115

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

    Plasmid

    #159281

    Purpose

    A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Insert

    AsCpf1-HA-2A-GFP
    

    Use

    Multicistronic vector with both caggs promoter-dr…

    Tags

    3X HA

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

    Plasmid

    #132962

    Purpose

    Firefly-luciferase single-strand annealing assay cloning backbone

    Depositor

    Gang Bao

    Article

    Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

    Insert

    Firefly Luciferase
    

    Use

    CRISPR and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Stop sequence between luciferase gene

    Promoter

    CMV

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET Flag TEV LIC cloning vector (1L)

    Plasmid

    #29662

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    Flag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge