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eGFP STAT1 S727E

Plasmid

#12305

Depositor

Alan Perantoni

Article

Timofeeva et al Oncogene. 2006 Dec 7;25(58):7555-64. doi

Insert

STAT1 S727E (STAT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S727E

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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TRPM4

Plasmid

#25903

Depositor

Craig Montell

Article

Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

Insert

TRPM4 (TRPM4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pHes1(2.5k)-luc

Plasmid

#43806

Depositor

Ryoichiro Kageyama

Article

Takebayashi et al J Biol Chem. 1994 Feb 18;269(7):5150-6.

Insert

Hes1 Promoter (Hes1 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains the murine Hes1 promoter

Promoter

Hes1 Promoter

Available Since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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MSCB-hDot1Lwt

Plasmid

#74173

Purpose

For packaging hDot1Lwt to retrovirus.

Depositor

Yi Zhang

Article

Okada et al Cell. 2005 Apr 22;121(2):167-78.

Insert

hDot1L (DOT1L Human)

Use

Retroviral

Tags

HA

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLJC5-LAMP1-RFP-2xFlag

Plasmid

#102931

Purpose

Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)

Depositor

David Sabatini

Article

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

LAMP1

Use

Lentiviral

Tags

mRFP-2xFlag

Promoter

ubc

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER dCas9-TET1CD

Plasmid

#101921

Purpose

The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

dCas9-TET1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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hAAVS1 TALEN Right

Plasmid

#52342

Purpose

TALEN vector for genomic targeting

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

AAVS1 TALEN Right

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2014

Availability

Academic Institutions and Nonprofits only

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hAAVS1 TALEN Left

Plasmid

#52341

Purpose

TALEN vector for genomic targeting

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

AAVS1 TALEN Left

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2014

Availability

Academic Institutions and Nonprofits only

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pQE30-His-CKM

Plasmid

#124134

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Creatine kinase (CKM Chicken)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4 Smad4

Plasmid

#16483

Depositor

Bert Vogelstein

Article

Riggins et al Nat Genet. 1996 Jul . 13(3):347-9.

Insert

Smad4 (SMAD4 Human)

Tags

Double HA

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pLKO.1.sh.beta-catenin.2279

Plasmid

#19762

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against beta-catenin (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA4-STRAD alpha

Plasmid

#14889

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

STRAD alpha (STRADA Human)

Tags

6xHis/Xpress

Expression

Mammalian

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
pMo130

Plasmid

#27388

Purpose

Burkholderia allelic exchange suicide vector pMo130, ref EU862243

Depositor

Martin Voskuil

Article

Hamad et al Gene. 2009 Feb 1. 430(1-2):123-31.

Type

Empty backbone

Use

Burkholderia allelic exchange suicide vector pmo1…

Available Since

March 7, 2012

Availability

Academic Institutions and Nonprofits only

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TRE-ChrimsonR-mCherry

Plasmid

#92207

Purpose

Reporter construct

Depositor

Alice Ting

Article

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Has Service

AAV1

Insert

ChrimsonR-mCherry

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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M-ST1-sgRNA

Plasmid

#48672

Purpose

Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter

Use

CRISPR

Promoter

hU

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pN3-Sp3FL

Plasmid

#24541

Depositor

Guntram Suske

Article

Sapetschnig et al J Biol Chem. 2004 Oct 1. 279(40):42095-1

Insert

transcription factor Sp3 (SP3 Human)

Expression

Mammalian

Available Since

June 16, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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Flag TSC2 wt

Plasmid

#12132

Depositor

Cheryl Walker

Article

Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Insert

TSC2 (TSC2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2006

Availability

Academic Institutions and Nonprofits only

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pET29b-IPP1-His

Plasmid

#124137

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Inorganic pyrophosphatase (IPP1 Budding Yeast)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5 Smad1-HA

Plasmid

#14956

Depositor

Joan Massague

Article

Liu et al Nature. 1996 Jun 13. 381(6583):620-3.

Insert

Smad1 (SMAD1 Human)

Tags

HA

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pSLQ9757: CMV-BPNLS_TadA-linkerA-TadA*(8e)-Linker B-SV40 NLS-dCasMINI-V4-linkerC-c-Myc_NLS-3x FLAG-polyA

Plasmid

#176270

Purpose

Expression of TadA-TadA* (8e)-dCasMINI-V4 in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

TadA-TadA*(8e)-dCasMINI-V4

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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