We narrowed to 8,727 results for: pcDNA3

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pcDNA3-Xl-VSP1 R152Q

Plasmid

#51881

Purpose

expression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 R152Q (tpte2 Frog)

Use

Xenopus oocyte expression

Expression

Mammalian

Mutation

R152Q: near the extracellular side of the fourth …

Promoter

CMV

Available Since

May 22, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1-wt

Plasmid

#12980

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Rac1 (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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HA-Elongin B-pcDNA3.1(+)-Zeo

Plasmid

#20000

Depositor

William Kaelin

Article

Ohh et al Nat Cell Biol. 2000 Jul . 2(7):423-7.

Insert

transcription elongation factor B (ELOB Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wild type

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PBD-EYFP

Plasmid

#13723

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

PBD-EYFP (PAK1 Human)

Tags

EYFP

Expression

Mammalian

Mutation

p21 binding domain of human Pak1(amino acids 65-1…

Available Since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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pcdna3 flag - ULK1 wt

Plasmid

#27636

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse ULK1 (Ulk1 Mouse)

Tags

flag

Expression

Mammalian

Mutation

Initial M from ULK1 removed.

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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WFS1-Flag-pcDNA3

Plasmid

#13011

Depositor

Fumihiko Urano

Article

Fonseca et al J Biol Chem. 2005 Nov 25. 280(47):39609-

Insert

Wolfram Syndrome 1 (WFS1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-300(HAT-)

Plasmid

#23254

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

p300 (EP300 Human)

Expression

Mammalian

Mutation

H1415A E1423A Y1424A L1428S Y1430A H1434A

Available Since

March 15, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dPlcR

Plasmid

#181832

Purpose

Genetically encoded single-color sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells. Based on dimerization-dependent green fluorescent protein (ddGFP).

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-CyclinF-ΔF

Plasmid

#32973

Depositor

Michele Pagano

Article

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Tags

FLAG

Expression

Mammalian

Mutation

F-box mutations: L35A and P36A

Promoter

CMV

Available Since

Feb. 3, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-RIPK3

Plasmid

#78815

Purpose

To overexpress RIPK3 in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-NP-B/BRISBANE/60/2008

Plasmid

#187921

Purpose

Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

Influenza B Nucleoprotein

Tags

UNTAGGED

Expression

Mammalian

Promoter

CMV Promoter

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-P2X2-YFP

Plasmid

#22400

Depositor

Henry Lester

Article

Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

Insert

ATP-gated P2X channel 2 (P2rx2 Rat)

Tags

YFP

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Floxed-STOP Fluc

Plasmid

#122962

Purpose

Firefly luciferase (Fluc)-based bioluminescent reporter for Cre-loxP DNA recombination reaction

Depositor

Moritoshi Sato

Article

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_Floxed-STOP Fluc

Use

Cre/Lox and Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mitoExRai-AKAR2

Plasmid

#161757

Purpose

ExRai-AKAR2 targeted to the outer mitochondrial membrane.

Depositor

Jin Zhang

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

DAKAP-ExRai-AKAR2

Tags

N-terminal 30 amino acids from DAKAP1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA2-CUL4A

Plasmid

#19907

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

Cullin 4A (CUL4A Human)

Tags

2xHA

Expression

Mammalian

Mutation

K644R

Promoter

CMV

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_T

Plasmid

#147556

Purpose

Mammalian Expression of HsSmg7

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7 (SMG7 Human)

Expression

Mammalian

Mutation

two silent mutation G216A, A1521G and one non sil…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7B2Jnk1a1

Plasmid

#19726

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

MKK7B2Jnk1a1 (Map2k7, MAPK8 Mouse, Human)

Tags

Flag

Expression

Mammalian

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RasAR-NES

Plasmid

#205567

Purpose

Genetically encoded FRET-based biosensor for monitoring cellular Ras GTPase activity. Targeted to cytosol.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

RasAR-NES (RAF1 Human, Synthetic)

Tags

Nuclear export signal (NES)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-dCry-V5His

Plasmid

#136241

Purpose

Protein expression of D. melanogaster (Dm) cytochrome with V5 and His tags

Depositor

Aziz Sancar

Article

Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

Insert

DmCry

Tags

His and V5

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-L11

Plasmid

#20936

Depositor

Yue Xiong

Article

Zhang et al Mol Cell Biol. 2003 Dec . 23(23):8902-12

Insert

Ribosomal protein L11 (RPL11 Human)

Tags

myc3

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only