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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,406 results for: puro

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Showing: 1661 - 1680 of 31406 results

  2. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid
    #164637
    Purpose
    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2
    Depositor
    Günter Schneider
    Insert
    target guide sequence PRMT5 inhibition (PRMT5 Human)
    Use
    CRISPR and Lentiviral; Interference
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164637-plasmid-map-sequence-id-326103

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  6. qTAG-N-Puro-mNeon-ACTB

    Plasmid
    #207752
    Purpose
    Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    207752-plasmid-map-sequence-id-414897

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  7. pLenti-xBE4Gam-P2A-Puro

    Plasmid
    #112676
    Purpose
    Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)
    Depositor
    Lukas Dow
    Article
    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419
    Insert
    xCas9(3.7)-BE4Gam
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…
    Promoter
    EF1s
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112676-plasmid-map-sequence-id-217196

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  9. pBabe puro IkB alpha M

    Plasmid
    #12332
    Depositor
    Inder Verma
    Insert
    IkB alpha M (Nfkbia Mouse)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    Dominant-negative mutant. Many signal transductio…
    Available Since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only

  18. pENTR-R2L3-IRES-puro-pA

    Plasmid
    #139529
    Purpose
    Provides IRES-puro-pA cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    puromycin resistant gene
    Expression
    Mammalian
    Available Since
    March 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    139529-plasmid-map-sequence-id-274646

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Showing: 1661 - 1680 of 31406 results