We narrowed to 23,083 results for: REP
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Plasmid#62893PurposeTrojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneAvailable SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only
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pT-GEM(2)
Plasmid#62894PurposeTrojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorTypeEmpty backboneAvailable SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70
Plasmid#62898PurposeCreating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2.DepositorInsertT2A-Gal4-Hsp70
Available SinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
PM-ABKAR
Plasmid#61506PurposeExpression of AMPK biosensor targeted to the plasma membrane via Lyn kinase targeting sequenceDepositorInsertAMPK Biosensor
TagsLyn kinase sequence (see comments)ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti- V6.3 lox-dsRED-stop-lox-eGFP-blas
Plasmid#106171Purposecre mediated red to green shiftDepositorInsertsdsRED-express2
eGFP
UseCre/Lox and LentiviralExpressionMammalianPromoterCMVAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pF CAG luc puro
Plasmid#67501PurposeConstitutive expression of firefly luciferase in mammalian cellsDepositorInsertFirefly luciferase
UseLentiviral and LuciferaseExpressionMammalianPromoterCAGAvailable SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
p5155
Plasmid#175391PurposeLow Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70
Plasmid#62914PurposeCreating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1DepositorInsertT2A-p65AD-Hsp70
Available SinceJuly 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(0)-T2A-Gal4DBD-Hsp70
Plasmid#62902PurposeCreating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0DepositorInsertT2A-Gal4DBD-Hsp70
Available SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
LAMP-mChF-AIP
Plasmid#61524PurposeExpression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIPDepositorInsertlysosomal-associated membrane protein 1 (LAMP1 Human)
TagsAIP and mCherryExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-dV-IgE/λ
Plasmid#61879PurposeHuman immunoglobulin epsilon antibody expression vector (lambda light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) coloniesDepositorInsertsHuman immunoglobulin Epsilon constant region
Human immunoglobulin lambda constant region
ExpressionMammalianMutationDeleted Variable regionPromotermEF1 Prom and rEF1 PromAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9-pAc
Plasmid#162163PurposeExpresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectableDepositorInsertshSpCas9
sgRNA
UseCRISPRTagsT2A-pAcExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A
Plasmid#154773Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
TagsFLAGExpressionMammalianMutationY125A in G1PylS, hybrid PylT with A41AA and C55A …PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TBK1-C-FLAG-HA
Plasmid#131792PurposeLentiviral vector expressing WT TBK1 containing STOP codonDepositorInsertTBK1 WT (TBK1 Human)
UseLentiviralTagsSTOP codonExpressionMammalianMutationWT full lengthPromoterCMVAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-mCherry-FLAG-cGAS E225A/D227A
Plasmid#127657PurposeOver-express catalytically inactive mCherry-FLAG-cGAS E225A/D227ADepositorAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCri-H6-TEV-GRAB-STREP
Plasmid#128499PurposeExpresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.DepositorInsertGRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)
TagsHis tag (6x) and Strep tag (2x)ExpressionBacterialPromoterT7Available SinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightG
Plasmid#187179PurposeExpresses green norepinephrine indicator nLightG in neuronal cellsDepositorHas ServiceAAV9InsertnLightG
UseAAVTagsFlag tagPromoterhuman Synapsin-1Available SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDest-Sumo
Plasmid#106980PurposeLR reaction to create expression plasmid. For prokaryotic expression of target protein as N-terminal fusion form with Sumo tagDepositorTypeEmpty backboneTagsHis8-SumoExpressionBacterialPromoterT7Available SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sox2-CreERT2
Plasmid#135618PurposeDonor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locusDepositorInsertP2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsP2A-CreERT2Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPPI4-Strep-SNAP-CD86-His10
Plasmid#223570PurposeProtein purification of human CD86 with Twin-Strep, SNAP and 10xHis tags from mammalian cellsDepositorAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only