We narrowed to 12,110 results for: NSI

Showing: 3441 - 3480 of 12110 results

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YFP-DHFR-myc

Plasmid

#118852

Purpose

For fluorophore translocation assay and inducible tagging with zapalog.

Depositor

Thomas Schwarz

Article

Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

Type

Empty backbone

Tags

DHFR, Myc, and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-CitroffRH

Plasmid

#158546

Purpose

Mammalian expression of nonbinding variant of inverse-response citrate sensor

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

CitroffRH

Expression

Mammalian

Promoter

cmv

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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CAG.CenpB(FL)-GFP-Halo

Plasmid

#176205

Purpose

Centromeric anchor for TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

CenpB-GFP-Halo

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Enhanced Repressor Domain for TAL Effector (SID4X)

Plasmid

#43882

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

SID4X Domain

Available Since

April 1, 2013

Availability

Academic Institutions and Nonprofits only
pECFP-DEVG-Venus

Plasmid

#24538

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-DEVG-Venus

Tags

ECFP and Venus

Expression

Mammalian

Mutation

DEVDR mutated to DEVG

Promoter

CMV

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
pKL07

Plasmid

#104431

Purpose

mitochondrial matrix targeted super ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vector

Depositor

Joel Kralj

Article

Dodd et al Sci Rep. 2017 Oct 24;7(1):13922. doi: 10

Insert

mito-Super Ecliptic pHluorin-mRuby2

Expression

Yeast

Promoter

Tef1

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 Mpro protease (M165I mutant - clinical mutant); aliases: 3CLpro, 3C-like

Plasmid

#204778

Purpose

Bacterial expression of Sars Cov 2 MPRO M165I mutant

Depositor

ASAP Discovery, Frank von Delft

Insert

Sars Cov 2 MPRO (ORF1ab Sars Cov 2)

Expression

Bacterial

Mutation

M165I

Available Since

Dec. 1, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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SmoCFP

Plasmid

#128156

Purpose

Expression of Smoothened

Depositor

Jennifer Lippincott-Schwartz

Article

Ott et al Cilia. 2012 Apr 25;1(1):3. doi: 10.1186/

Insert

Smoothened (Smo Mouse)

Expression

Mammalian

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Myc-hZO-1-HA

Plasmid

#236528

Purpose

For expression of ZO-1 (human) in mammalian cells

Depositor

Sandra Citi

Article

Spadaro et al Curr Biol. 2017 Dec 18;27(24):3783-3795.

Insert

TJP1 (TJP1 Human)

Tags

HA and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-mCherry-C1-WPRE

Plasmid

#159956

Purpose

untargeted marker

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

mCherry-C1

Use

AAV

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Tus pGST-//

Plasmid

#53305

Purpose

Expresses GST-tagged E.coli replication terminator protein Tus.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

E.Coli Tus replication terminator protein

Tags

GST

Expression

Bacterial

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-3FLAG

Plasmid

#221045

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xFLAG epitopes

Use

Unspecified

Promoter

read through

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-R-Kras

Plasmid

#180572

Purpose

ddRFP sensor of Kras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Kras

Expression

Mammalian

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQAT03

Plasmid

#223375

Purpose

T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-SpCas9-AtU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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ELK1 pcDNA3.1/HisC

Plasmid

#121093

Purpose

Expression of full length ELK1 in mammalian cells

Depositor

Paul Herring

Article

Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

Insert

ELK1 (Elk1 Mouse)

Tags

6XHis-Omni-xpress

Expression

Mammalian

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pECFP-IETD2x-Venus

Plasmid

#24536

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-IETD2x-Venus

Tags

ECFP and Venus

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
pmNeonGreenHO-mito

Plasmid

#127913

Purpose

a mammalian expression vector for mitochondria-targeting humanized mNeonGreen

Depositor

Isei Tanida

Article

Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

Insert

humanized mNeonGreen with mitocondrial targetting signal

Tags

mNeonGreen

Expression

Mammalian

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pEM705 Gso (Cys)

Plasmid

#109375

Purpose

GsX chimera with Gαo C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gso chimera

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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PNMT

Plasmid

#64078

Purpose

Expression of human Phenylethanolamine N-methyltransferase

Depositor

Vern Schramm

Article

Stratton et al ACS Chem Biol. 2017 Feb 17;12(2):342-346

Insert

Phenylethanolamine N-methyltransferase (PNMT Synthetic, Human)

Tags

Histidine tag

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only
pCHW110031

Plasmid

#222437

Purpose

Level 0, promoter module (GGAG-CCAT) containing CBS4 and CMV1 promoters.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pT7T-EcadTSMod

Plasmid

#99871

Purpose

RNA synthesis for microinjection into xenopus laevis embryo

Depositor

Marc Tramier

Article

Herbomel et al Sci Rep. 2017 Mar 22;7:45058. doi: 10.10

Insert

EcadTSMod

Use

Xenopus microinjection

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-GCaMP6s

Plasmid

#184712

Purpose

Calcium biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

GCaMP6s

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCHERRY10

Plasmid

#24664

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

mCherry

Expression

Bacterial

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

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pRep

Plasmid

#199599

Purpose

Expresses AAV2 rep gene; accessory plasmid for the pCMV-Cap SSM library.

Depositor

George Church

Article

Ogden et al Science. 2019 Nov 29;366(6469):1139-1143

Insert

AAV2 rep

Use

AAV

Expression

Mammalian

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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H2B-mScarlet- hMeikin(1-332)-mNeonGreen

Plasmid

#174714

Purpose

Stable expression of fluorescent Separase cleavage sensor with human Meikin

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Use

Retroviral

Tags

H2B-mScarlet and mNeonGreen

Expression

Mammalian

Mutation

1-332

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pMJ106: pRS426-His-MBP-NlovFz2_Native

Plasmid

#205253

Purpose

S. cerevisiae expression vector for NlovFz2 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-GFP(LaG16) PAGER(Gs)

Plasmid

#230007

Purpose

Expresses anti-GFP PAGER(Gs) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaG16-TEVcs-ALFA-hM1Dxs

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET_RFP-SZ2

Plasmid

#124933

Purpose

Expression of RFP-SZ2

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RFP-SYNZIP2

Tags

His and mCherry

Expression

Bacterial

Mutation

SZ2 domain

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBW-507

Plasmid

#195469

Purpose

Lentiviral Integratable Constitutive CD19CAR

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV-CD19CAR-mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEF/myc/ER FLII12Pglu-700uDelta6

Plasmid

#17867

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLII12Pglu-700uDelta6

Tags

Citrine

Expression

Mammalian

Mutation

700uDelta6, ECFP insert in mglB

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pCHW100002

Plasmid

#222440

Purpose

Level 0, CDS module (AATG-GCTT) containing rice OsL5.

Depositor

Chih-Hang Wu

Article

Chiang et al bioRxiv 2024.03.23.586378

Insert

OsL5 (Oryza sativa)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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ROPY (sYFP2-OP18-mScI-R125I)

Plasmid

#128002

Purpose

Improved redshifted FRET-readout of Stathmin/OP18 interaction with α-tubulin

Depositor

Dorus Gadella

Article

Van Geel et al Sci Rep. 2020 Apr 7;10(1):6034. doi: 10.

Insert

sYFP2-OP18-mScI-R125I

Expression

Mammalian

Mutation

R125I in mScarlet-I

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-GFP-gfp_pJMP2768

Plasmid

#208889

Purpose

Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette, sfGFP

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHS-435

Plasmid

#195470

Purpose

Lentiviral Integratable Constitutive super IL-2 cytokine

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pEF1a-Super IL-2-2A-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv6-puro-3Ty:tagRFPt:3Ty

Plasmid

#201089

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
VV020: WT Arch-3 - eGFP in pET28b

Plasmid

#58488

Purpose

Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

Depositor

Adam Cohen

Article

Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Archaerhodopsin-3

Tags

eGFP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B

Plasmid

#157809

Purpose

Expression of human histone H2B

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B

Expression

Bacterial

Promoter

T7

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCri_L706-antiSH2-OptoMB

Plasmid

#159935

Purpose

Opto-MB bacterial expression

Depositor

Jose Avalos, Jared Toettcher

Article

Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

Insert

anti-SH2 MB with SS58 insert

Expression

Bacterial

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX2T-GATA3

Plasmid

#1290

Depositor

Gokhan Hotamisligil

Article

Tong et al Science 2000 Oct 6;290(5489):134-8.

Insert

GATA3 (Gata3 Mouse)

Tags

GST

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pU1A_full-myc-T2A-tagRFP

Plasmid

#140264

Purpose

Expresses U1A in mammalian cells

Depositor

Hirohide Saito

Article

Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

Insert

U1A

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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