We narrowed to 24,871 results for: Spr

1. pTol2-HybridBarcode

   Plasmid

   #174037

   Purpose

   For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

   Depositor

   James Gagnon

   Article

   Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

   Insert

   CRISPR target sites
   

   Use

   Tol2 transgenesis

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDYSCKO

   Plasmid

   #120263

   Purpose

   Empty backbone for cloning any gRNA to be used with the canavanine co-selection system

   Depositor

   Christian Landry

   Article

   Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

   Insert

   DYSCKO cassette
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   SNR52

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBbdCas9S_P0526-sgRNA500

   Plasmid

   #149641

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30, P0526

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbdCas9S(RBSmut)

   Plasmid

   #149574

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBbdCas9S(-10TC)

   Plasmid

   #149581

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRoCascade_gfp

   Plasmid

   #178771

   Purpose

   Expression of Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis

   Depositor

   Chase Beisel

   Article

   Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

   Insert

   Rocas6, Rocas8, Rocas7, Rocas5, RoCAST CRISPR repeat, gfp from Rippkaea orientalis
   

   Expression

   Bacterial

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pADH118-2

   Plasmid

   #90984

   Purpose

   NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. albicans LEU2 2 of 2
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459V2.0+K848A

   Plasmid

   #108296

   Purpose

   pX459 V2.0 (Plasmid #62988) with the K848A mutation

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbdCas9S_arr2

   Plasmid

   #149639

   Purpose

   parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   PflaB, PpQE30

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ291

    Plasmid

    #129671

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    BthCas12b
    

    Use

    CRISPR; Gateway compatible bthcas12b entry clone

    Expression

    Plant

    Mutation

    BthCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbdCas9G_arr2

    Plasmid

    #149571

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbdCas9G(RBSmut)_arr2

    Plasmid

    #149578

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbdCas9K(-10TC)_arr2

    Plasmid

    #149584

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbdCas9K_arr2

    Plasmid

    #149570

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBbdCas9K(RBSmut)_arr2

    Plasmid

    #149577

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pADH143-2

    Plasmid

    #90990

    Purpose

    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. maltosa LEU2 2 of 2
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pADH100-2

    Plasmid

    #90981

    Purpose

    NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Insert

    NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, FRT, DS_HIS1
    

    Use

    CRISPR

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCK321

    Plasmid

    #242912

    Purpose

    aTc-inducible expression of dCas9-AsiA and constitutive expression of MCP-SoxS

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

    Insert

    MCP-SoxS
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    BBa_J23107

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Modular BE4max for GG insertion

    Plasmid

    #198891

    Purpose

    Easily insert deaminase of choice into BE4max using Golden Gate cloning

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    CRISPR associated protein 9 (cas9 Streptococcus pyogenes)
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28b_TypeIC_acrIF2_invivo

    Plasmid

    #196402

    Purpose

    expresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assay

    Depositor

    David Taylor

    Article

    O'Brien et al Mol Cell. 2023 Mar 2;83(5):746-758.e5. d

    Insert

    anti-CRISPR IF2 protein (orf30 P. aeruginosa)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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