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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 1761 - 1780 of 67219 results

  5. M-SP-sgRNA

    Plasmid
    #48671
    Purpose
    Mammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTG
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. pyogenes Cas9, hU6 promoter
    Use
    CRISPR
    Promoter
    hU6
    Available since
    Nov. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48671-plasmid-map-sequence-id-71841

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  7. PDIA3-bio-His

    Plasmid
    #52070
    Purpose
    Expresses full-length Protein disulfide-isomerase A3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PDIA3 (PDIA3 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52070-plasmid-map-sequence-id-270198

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  9. pCMV-Spike

    Plasmid
    #173921
    Purpose
    Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter
    Depositor
    Andreas Stuermer
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-2 Spike Protein (S Synthetic, Human, SARS-COV-2)
    Tags
    no tags, native protein
    Expression
    Bacterial and Mammalian
    Promoter
    standard CMV
    Available since
    April 11, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    173921-plasmid-map-sequence-id-358849

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  10. pmRFP-N1 human cofilin R21Q

    Plasmid
    #51279
    Purpose
    expresses human cofilin R21Q mutant fused to mRFP in mammalian cells
    Depositor
    James Bamburg
    Publication
    Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cofilin 1 (CFL1 Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    R21Q, non-rod-forming mutation that can be used t…
    Promoter
    CMV
    Available since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  11. pdCas9-DNMT3A-PuroR (ANV)

    Plasmid
    #71684
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71684-plasmid-map-sequence-id-129672

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  12. mCherry-EB3-7

    Plasmid
    #55037
    Purpose
    Localization: MT End Binding Protein, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EB3 (MAPRE3 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    55037-plasmid-map-sequence-id-269079

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  13. SARS-CoV-2 Spike-d18

    Plasmid
    #164583
    Purpose
    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.
    Depositor
    Jason Sheltzer
    Insert
    SARS-CoV-2 Spike (S SARS-CoV-2)
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1256-1273
    Promoter
    EF1a
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    164583-plasmid-map-sequence-id-322362

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  15. pcDNA3.3_CoV2_D18

    Plasmid
    #170442
    Purpose
    Express SARS-CoV-2 WT spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 pseudotyped virus production.
    Depositor
    David Nemazee
    Publication
    Rogers et al Science. 2020 Aug 21;369(6506):956-963.
    Insert
    SARS-CoV-2 spike D18 (S SARS-CoV-2, USA-WA1/2020 strain)
    Tags
    NA
    Expression
    Mammalian
    Mutation
    18aa deletion in c-terminal tail
    Promoter
    CMV
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170442-plasmid-map-sequence-id-337888

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  16. pmRFP-N1 human cofilin S3A

    Plasmid
    #50857
    Purpose
    expresses constituvely active cofilin fused to mRFP in mammalian cells
    Depositor
    James Bamburg
    Publication
    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cofilin 1 S3A (CFL1 Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    S3A constitutively active
    Promoter
    CMV
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  20. pmRFP-N1 human cofilin S3E

    Plasmid
    #50858
    Purpose
    expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells
    Depositor
    James Bamburg
    Publication
    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cofilin 1 S3E (CFL1 Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    S3E psuedo-phosporylated, non-activatable
    Promoter
    CMV
    Available since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 1761 - 1780 of 67219 results