We narrowed to 4,284 results for: 261
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Plasmid#169924PurposeWT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2DepositorInsertEcm2 1-197
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFlox_N-BRM_BSD_FKBPF36V_EYFPF36V
Plasmid#163876PurposeAcute Depletion of BRM-EYFPDepositorInsertBrm (Smarca2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-rab23-DN
Plasmid#109734PurposeMultisite gateway vector for 3' tagging with dominant negative rab23DepositorInsertrab23 (rab23 Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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actc1b-mKate2-rab23
Plasmid#109612PurposeMuscle specific zebrafish expression of mKate2 fused to rab23DepositorInsertrab23 (rab23 Zebrafish)
UseZebrafish plasmidsTagsmKate2ExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p1147-pCSDest-AcrIIC3Nme-mCherry-miR122BS
Plasmid#126203PurposeMammalian expression of AcrIIC3Nme fused to mCherry with 3x miR-122 binding sites in 3' UTRDepositorInsertcodon-optimized AcrIIC3Nme
UseTagsFLAG/NLS and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
p1149-pCSDest-AcrIIA4-mCherry-miR122BS
Plasmid#126205PurposeMammalian expression of AcrIIA4 fused to mCherry with 3x miR-122 binding sites in 3' UTRDepositorInsertcodon-optimized AcrIIA4
UseTagsFLAG/NLS and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 N4A 3xFLAG
Plasmid#111975PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation.DepositorInsertHsh155 N4A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationT178A R181A R182A R186APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K818A 3xFLAG
Plasmid#111982PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains K818A mutation. LethalDepositorInsertHsh155 K818A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationK818APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R775A 3xFLAG
Plasmid#111977PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. LethalDepositorInsertHsh155 R775A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationR775APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R778A 3xFLAG
Plasmid#111979PurposeShuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R778A mutation. LethalDepositorInsertHsh155 R778A 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationR778APromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
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LLhE_AW-pHG165 H118R
Plasmid#90063PurposeConstitutive expression of a chimeric LacI:CelR transcription repressorDepositorInsertLLhE_AW/H118R
UseTagsExpressionBacterialMutationI48V/Q55A/Q60R/H118RPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LLhG-pHG165a
Plasmid#90039PurposeConstitutive expression of a chimeric LacI:GalR transcription repressorDepositorInsertLLhG
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
LGhG-pHG165a
Plasmid#90048PurposeConstitutive expression of a chimeric LacI:GalR transcription repressorDepositorInsertLGhG
UseTagsExpressionBacterialMutationPromoterIqAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF1G12
Plasmid#70860PurposeGateway entry cloneDepositorInsertmrn (mrn Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 thru gap + Cys
Plasmid#49161PurposeTemplate for generating gene 60 mRNA truncated two codons after coding gap + CysDepositorInsertBacteriophage T4 gene 60
UseTagsExpressionMutationgene 60 mRNA truncated two codons after coding ga…PromoterT7Available sinceFeb. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
TMEM50B_pCSdest
Plasmid#53835DepositorInsertTMEM50B (TMEM50B Human)
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceSept. 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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psiCHECK RHEBL1 3'UTR
Plasmid#19858DepositorInsertRHEBL1 3'UTR (RHEBL1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-143
Plasmid#169923PurposeWT Ecm2 mutated to introduce stop codon after AA 143 of Ecm2DepositorInsertEcm2 1-143
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-265
Plasmid#169925PurposeWT Ecm2 mutated to introduce stop codon after AA 265 of Ecm2DepositorInsertEcm2 1-265
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-325
Plasmid#169926PurposeWT Ecm2 mutated to introduce stop codon after AA 325 of Ecm2DepositorInsertEcm2 1-325
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pIZ-Flag6His-Siwi
Plasmid#50560PurposeExpresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cellsDepositorInsertSiwi (Aubergine )
UseTags6xHIS and FLAGExpressionInsectMutationPromoterOpIE2Available sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB_tre_Cas9
Plasmid#126029PurposePiggyBac cargo vector expressing doxycycline inducible Cas9DepositorInsertshumanized S. pyrogenes Cas9
Hygromycin resistance
UseCRISPR; PiggybacTagsExpressionMammalianMutationPromoterEF1 alpha and TREAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
GA-NES
Plasmid#61018PurposeddGFP A-NESDepositorInsertddGFP A
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-SUMO-MarathonRT
Plasmid#109029PurposeGroup II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”DepositorInsertERE_01490
UseTags6xHis-SUMO tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorInsertAdenosine deaminase acting on RNA (Adar Fly)
UseTagsV5ExpressionBacterial and InsectMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHrD-IRES-Fluc
Plasmid#194250PurposeHuman RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterhuman rDNA promoter and beginning of 5' ETS …Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLR16-Phes*
Plasmid#98783PurposeSuicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).DepositorInsertpheS
UseTagsExpressionBacterialMutationA309GPromoterAvailable sinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CamKIIa_GFP
Plasmid#96941PurposeTo label mature excitatory iPSC-derived human neuronsDepositorInsertGreen fluorescent protein
UseLentiviralTagsExpressionMutationPromoterCaMKIIaAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-EGFP-Donor
Plasmid#159740PurposeContains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.DepositorInsertEGFP
UseIntron tagging donorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pphiLOV3-N1
Plasmid#178973PurposeExpresses phiLOV3 in mammalian cellsDepositorInsertphiLOV3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GB-NES
Plasmid#61017PurposeddGFP B-NESDepositorInsertddGFP B
UseTagsHindIII site and NES sequence LALKLAGLDIGSExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-calpainsensor
Plasmid#36182Purposedetection of activity of ubiquitous calpainsDepositorInsertalpha-Fodrin cleavage site (Sptan1 Mouse)
UseTagseCFP and eYFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
CaM1234(D20A/D56AD93A/D129A)/pIRES2-eGFP
Plasmid#111518PurposeElectrophysiology in HEK cellDepositorInsertcalmodulin [Xenopus laevis] (calm2 Frog)
UseTagsExpressionMammalianMutationD20A/D56AD93A/D129APromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIN-Arkadia-gRNA1
Plasmid#180374Purposetargeting mouse Arkadia geneDepositorInsertArkadia targeting gRNA (Rnf111 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-MCS-NLS-3XFLAG-V5
Plasmid#196383PurposeEmpty backbone vector to insert cDNA for strong expression in mammalian cellsDepositorTypeEmpty backboneUseTags3xFLAG and V5ExpressionBacterial and MammalianMutationPromoterCAGGsAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIZ-Flag6His-BmAgo3
Plasmid#50559PurposeExpresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cellsDepositorInsertBmAgo3
UseTags6xHIS and FlagExpressionInsectMutationPromoterOpIE2Available sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUB7
Plasmid#169698PurposeTemplate plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
UseTags6xHis tagExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only