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Addgene

We narrowed to 4,284 results for: 261

Showing: 3641 - 3680 of 4284 results
  1. Hsh155 N4A 3xFLAG

    Plasmid
    #111975
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker. It has 3xFLAG at the C-terminus. Contains the N4A mutation.
    Depositor
    Insert
    Hsh155 N4A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    T178A R181A R182A R186A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  2. Hsh155 K818A 3xFLAG

    Plasmid
    #111982
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains K818A mutation. Lethal
    Depositor
    Insert
    Hsh155 K818A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    K818A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. Hsh155 R775A 3xFLAG

    Plasmid
    #111977
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R775A mutation. Lethal
    Depositor
    Insert
    Hsh155 R775A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    R775A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. Hsh155 R778A 3xFLAG

    Plasmid
    #111979
    Purpose
    Shuffle plasmid containing the HSH155 gene and surrounding regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus. Contains R778A mutation. Lethal
    Depositor
    Insert
    Hsh155 R778A 3x FLAG
    Use
    Tags
    3xFLAG
    Expression
    Bacterial and Yeast
    Mutation
    R778A
    Promoter
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. BDH2

    Plasmid
    #39151
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    BDH2 (BDH2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  6. pMT-ADARcd-E488Q-V5

    Plasmid
    #139686
    Purpose
    Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5
    Depositor
    Insert
    Adenosine deaminase acting on RNA (Adar Fly)
    Use
    Tags
    V5
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pHrD-IRES-Fluc

    Plasmid
    #194250
    Purpose
    Human RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!
    Depositor
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    human rDNA promoter and beginning of 5' ETS …
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pLR16-Phes*

    Plasmid
    #98783
    Purpose
    Suicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).
    Depositor
    Insert
    pheS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A309G
    Promoter
    Available since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pSUB7

    Plasmid
    #169698
    Purpose
    Template plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Insert
    6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 3641 - 3680 of 4284 results