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Showing: 4551 - 4600 of 4641 results

  2. MCS-3_Endofin_LgBiT

    Plasmid
    #182574
    Purpose
    This plasmid can be used to monitor receptor internalization in real time living cells using bioluminescence as a readout.
    Depositor
    Bradley McConnell
    Article
    Reyes-Alcaraz et al Commun Biol. 2022 Mar 8;5(1):212. doi: 1
    Insert
    zinc finger FYVE-type (ZFYVE16 Human)
    Use
    Tags
    LgBiT (Large BiT)
    Expression
    Mammalian
    Mutation
    FYVE domain Q739-K806
    Promoter
    Herpes simplex virus (HSV)
    Available since
    Nov. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    182574-plasmid-map-sequence-id-450120

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  3. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid
    #170977
    Purpose
    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.
    Depositor
    Miki Ebisuya
    Article
    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:
    Insert
    CShroom3 (Shroom3 Mouse)
    Use
    Gateway cloning
    Tags
    SspB and mCherry
    Expression
    Mutation
    Isoform X2, deleted aminoacids 1 - 1388
    Promoter
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170977-plasmid-map-sequence-id-336791

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  6. TFORF3311

    Plasmid
    #144787
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CREB3 (CREB3 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    144787-plasmid-map-sequence-id-391511

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  8. pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR

    Plasmid
    #207805
    Purpose
    E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.
    Depositor
    Koen Breyne
    Article
    Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. do
    Insert
    E-NoMi
    Use
    Lentiviral
    Tags
    3xFlag tag, copGFP, mCherry, and nanoluciferase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    207805-plasmid-map-sequence-id-419158

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  9. pUAS-Myc-BirA-G3-ER

    Plasmid
    #173815
    Purpose
    Promiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site
    Depositor
    Norbert Perrimon
    Article
    Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:
    Insert
    Myc-BirA-G3-ER
    Use
    Tags
    Myc
    Expression
    Insect
    Mutation
    Promoter
    UAS
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    173815-plasmid-map-sequence-id-340316

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  12. pCIBN-hTRF2-tagRFP-T

    Plasmid
    #103812
    Purpose
    BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-hTRF2-tagRFP-T (CIB1, TERF2 Mustard Weed, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hTRF2: deletion of amino acids 1-42 and 476 compa…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103812-plasmid-map-sequence-id-199277

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  14. pCIBN-tagBFP-hNCL

    Plasmid
    #103805
    Purpose
    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-tagBFP-hNCL (CIB1, NCL Mustard Weed, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103805-plasmid-map-sequence-id-199237

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  15. pCIBN-LacI

    Plasmid
    #103814
    Purpose
    BLInCR 'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-LacI (CIB1, lacI Mustard Weed, E. coli)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    LacI: C19T (silent, L7L), T264C (silent, A88A), C…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103814-plasmid-map-sequence-id-199324

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  16. pCIBN-EYFP-hNCL

    Plasmid
    #103806
    Purpose
    BLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue light
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-EYFP-hNCL (CIB1, NCL Mustard Weed, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hNCL: A395G (K132R), A421G (K141E), A424G (K142E)…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103806-plasmid-map-sequence-id-199240

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  17. pGECPL.BC10.MG

    Plasmid
    #223822
    Purpose
    Contains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.
    Depositor
    Dawid Nowak
    Article
    Serio et al Cancer Discov. 2024 Jul 5. doi: 10.1158/
    Insert
    Ef1a-Driven Cre-P2A-FLuc
    Use
    CRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…
    Tags
    P2A
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    223822-plasmid-map-sequence-id-443651

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  19. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

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  20. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid
    #99278
    Purpose
    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mVenus-IRES-mCherry
    Puro
    Use
    Lentiviral
    Tags
    H2B, IRES, PGK, mCherry, and mVenus
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99278-plasmid-map-sequence-id-262659

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  26. pCIBN-LacI-tagRFP-T

    Plasmid
    #103810
    Purpose
    'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-LacI-tagRFP-T (CIB1, lacI Mustard Weed, E. coli)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    LacI: C19T (silent, L7L), T264C (silent, A88A), C…
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103810-plasmid-map-sequence-id-199266

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  28. pCIBN-hTRF1-tagRFP-T

    Plasmid
    #103811
    Purpose
    BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment
    Depositor
    Karsten Rippe
    Article
    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422
    Insert
    CIBN-hTRF1-tagRFP-T (CIB1, TERF1 Mustard Weed, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    103811-plasmid-map-sequence-id-199270

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  30. pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL

    Plasmid
    #73209
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    kappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Synthetic, Human)
    Use
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73209-plasmid-map-sequence-id-139734

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  40. AiP13785 - pAAV-AiE0459m-minBG-iCre(R297T)-BGHpA (Alias: CN3785)

    Plasmid
    #224037
    Purpose
    AiE0459m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons (note HPF subiculum pyr)
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    iCre(R297T)
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    minBG
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    224037-plasmid-map-sequence-id-462742

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  41. AiP13032 - pAAV-AiE0464m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3032)

    Plasmid
    #224024
    Purpose
    AiE0464m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons (note HPF subiculum pyr; MB dopaminergic)
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    minBG
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    224024-plasmid-map-sequence-id-450594

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  42. AiP13027 - pAAV-AiE0461m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3027)

    Plasmid
    #224021
    Purpose
    AiE0461m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L2/3_IT, L5_ET neurons (note HPF subiculum pyr)
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    minBG
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    224021-plasmid-map-sequence-id-450590

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  43. AiP13015 - pAAV-AiE0452m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3015)

    Plasmid
    #224018
    Purpose
    AiE0452m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons (note HPF subiculum pyr)
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    minBG
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    224018-plasmid-map-sequence-id-450578

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  44. AiP13898 - pAAV-AiE0647m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3898)

    Plasmid
    #224039
    Purpose
    AiE0647m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons (note HPF subiculum pyr; MB dopaminergic)
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    minBG
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    224039-plasmid-map-sequence-id-449789

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  45. AiP13319 - pAAV-AiE0583m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3319)

    Plasmid
    #224028
    Purpose
    AiE0583m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons (note HPF subiculum pyr; MB dopaminergic)
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    minBG
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    224028-plasmid-map-sequence-id-449782

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  46. AiP12250 - pAAV-AiE0452m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2250)

    Plasmid
    #223975
    Purpose
    AiE0452m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons (note HPF subiculum pyr)
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.
    Insert
    SYFP2
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    minBG
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    223975-plasmid-map-sequence-id-449752

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  47. FREQ-Seq Kit

    Plasmid Kit
    #1000000032
    Purpose
    For construction of barcoded, locus-specific libraries compatible with Illumina next generation sequencing. Allows for direct quantitative allele detection. 48 plasmid adaptors (barcoded M13F primer)
    Depositor
    Christopher Marx
    Available since
    May 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  48. MXS Chaining Kit

    Plasmid Kit
    #1000000064
    Purpose
    MXS-chaining system for assembly of constructs optimized for synthetic biology applications in mammalian systems. 14 different fluorescent proteins, 10 mammalian promoters/enhancers, 3 polyA signals
    Depositor
    Pierre Neveu
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  49. Diatom uLoop Assembly Kit

    Plasmid Kit
    #1000000238
    Purpose
    The collection contains a selection of diatom promoters and terminators from the organism Phaeodactylum tricornutum on vectors compatible with the uLoop cloning system.
    Depositor
    Christopher Dupont
    Available since
    Feb. 26, 2024
    Availability
    Academic Institutions and Nonprofits only

  50. FNL Combinatorial Cloning Platform

    Plasmid Kit
    #1000000211
    Purpose
    A collection of Multisite Gateway Entry clone plasmids which can be used in a combinatorial fashion to generate large numbers of tagged expression clones for protein expression in a variety of systems
    Depositor
    Dominic Esposito
    Available since
    Dec. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 4551 - 4600 of 4641 results