Purpose
Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR
Inserts
tef1 promoter - xylulose kinase - pex20 terminator
pyk1 promoter - xylose reductase - pex16 terminator
gapdh promoter - xylitol dehydrogenase - lip2 terminator
Use
Easycloneyali insert vector
Promoter
gapdh, pyk1, and tef1
Available Since
March 29, 2021
Availability
Academic Institutions and Nonprofits only