We narrowed to 23,544 results for: CHI

1. pOP462

   Plasmid

   #85935

   Purpose

   Encodes the Anti-Ebola 13C6 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

   Depositor

   Timothy Lu

   Article

   Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

   Insert

   Anti-Ebola 13C6 monoclonal antibody
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAGT8162 (AATG_dttLbCas12a-i_TTCG)

   Plasmid

   #192350

   Purpose

   L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   AATG_dttLbCas12a-i_TTCG
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D156R; E925Q

   Promoter

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.2/V5 hsa-mir-214

   Plasmid

   #26313

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   hsa-mir-214 (MIR214 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pCRIS

   Plasmid

   #120424

   Purpose

   This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.

   Depositor

   Tamás Fehér

   Article

   Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

   Insert

   CRISPR spacers targeting IS1, IS5, IS3, IS150
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   constitutive

   Available since

   Jan. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCRISP_IS

   Plasmid

   #120425

   Purpose

   This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

   Depositor

   Tamás Fehér

   Article

   Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

   Insert

   CRISPR spacers targeting IS1, IS5, IS3
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   constitutive

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHA-ΔN335-Kin1A

   Plasmid

   #31606

   Depositor

   Anthony Brown

   Article

   Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

   Insert

   ΔN335 kinesin-1A (Kif5a Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-335

   Promoter

   CMV

   Available since

   Oct. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

7. LentiU6-hNEUROD1 gRNA_1-MS2-Puro

   Plasmid

   #192676

   Purpose

   Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human NEUROD1 activating gRNA #1 (NEUROD1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPagP

   Plasmid

   #112125

   Purpose

   Bacterial expression of outer membrane protein PagP, wild type

   Depositor

   Karen Fleming

   Article

   Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

   Insert

   PagP (pagP E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N-terminal signal sequence not included and repla…

   Promoter

   T7

   Available since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA RBD-GFP11

   Plasmid

   #133069

   Purpose

   Expresses RBD domain with tripartite split GFP11 (M4)

   Depositor

   Stéphanie Cabantous

   Article

   Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

   Insert

   RBD (Rhotekin-Rho Binding Domain)
   

   Use

   Tags

   GFP11 (tripartite split-GFP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. THI VP64 pGAP T-

    Plasmid

    #161487

    Purpose

    Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Non targeting control NTC)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.2/V5 mmu-mir-375

    Plasmid

    #26336

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-375 (Mir375 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.2/V5 mmu-mir-137

    Plasmid

    #26327

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-137 (Mir137 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pICSL80036

    Plasmid

    #68259

    Purpose

    Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Coding sequence, hygromycin phophotransferase II (Escherichia coli)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pExT

    Plasmid

    #36889

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (i.e., ATG, start codon as a first exon)
    GFP
    beta-globin intron
    Hygro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides before nucleotide 275 and ins…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR1A-3xFlag-M694V-MEFV

    Plasmid

    #167118

    Purpose

    Gateway entry plasmid with 3xFlag-pyrin (M694V/FMF variant)

    Depositor

    Thomas Henry

    Article

    Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

    Insert

    MEFV (MEFV Human)
    

    Use

    Gateway entry vector

    Tags

    3xFlag

    Expression

    Mutation

    M694V Methionine 694 to valine (FMF mutation)

    Promoter

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pExG

    Plasmid

    #36888

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    Hygro
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KA1396_pGL4-UBCmin-luc2/Klf4-E'

    Plasmid

    #124187

    Purpose

    Luciferase reporter driven by the Klf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBSSK p85 alpha (delta SH3)

    Plasmid

    #13430

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (delta SH3) (PIK3R1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    SH3 domain replaced by flag tag.

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pOP461

    Plasmid

    #85934

    Purpose

    Encodes the Anti-Ebola 4G7 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

    Depositor

    Timothy Lu

    Article

    Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

    Insert

    Anti-Ebola 4G7 monoclonal antibody
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. BirA_BVES untagged

    Plasmid

    #196581

    Purpose

    expresses BirA

    Depositor

    Cheryl Arrowsmith

    Article

    Alteen et al Structure. 2023 Sep 7;31(9):1121-1131.e6

    Insert

    BirA_Ecoli
    

    Use

    Baculovirus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pTAL-MajSat-FLAG-NLS-MCS

    Plasmid

    #69073

    Purpose

    Expresses TALE for mouse major satellite with FLAG tag under CAG promoter

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

    Insert

    TALE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. px330-UFSP2 sgRNA2

    Plasmid

    #134637

    Purpose

    contains sgRNA targeting human UFSP2 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFSP2 sgRNA2 (UFSP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGBW-m4046925

    Plasmid

    #145694

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pcDNA3.2/V5 hsa-mir-128

    Plasmid

    #26308

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-128 (MIR128-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

25. KA2626_pGL4-UBCmin-luc2/Klf2-D'

    Plasmid

    #124199

    Purpose

    Luciferase reporter driven by the Klf2 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf2 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.2/V5 hsa-mir-193b

    Plasmid

    #26318

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-193b (MIR193B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

27. PM18033-PLS-KP6-A66S_rpoA-U

    Plasmid

    #190989

    Purpose

    Test the U-to-C editing activity of KP6:A66S on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    DYW-KP:A66S

    Promoter

    CMV

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PM18033-PLS-KP2-A66S_rpoA-U

    Plasmid

    #190977

    Purpose

    Test the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in human cells

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP2
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    DYW-KP:A66S

    Promoter

    CMV

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP ratAPOBEC1 L173A/G227A

    Plasmid

    #167169

    Purpose

    Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Leucine 173 to Alanine; changed Glycine 2…

    Promoter

    CMV promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. KA1401_pGL4-UBCmin-luc2/Klf4-F'

    Plasmid

    #124188

    Purpose

    Luciferase reporter driven by the Klf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. KA1395_pGL4-UBCmin-luc2/Klf4-B'

    Plasmid

    #124186

    Purpose

    Luciferase reporter driven by the Klf4 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Klf4 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBSSK p85 alpha (delta BH)

    Plasmid

    #13431

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (delta BH) (PIK3R1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    BH domain replaced by flag tag.

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

33. NET33 pmRFP-N2 (448)

    Plasmid

    #61979

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET33 (SCARA5 Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

34. LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro

    Plasmid

    #192681

    Purpose

    Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Mouse Hbb-bh1 activating gRNA #1 (Hbb-bh1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PKG I beta CAT

    Plasmid

    #16396

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 beta (PRKG1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    CAT: catalytic domain

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

36. NKP33 GFP (1032)

    Plasmid

    #62022

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    C9orf46 (PLGRKT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pOmpT

    Plasmid

    #112123

    Purpose

    Bacterial expression outer membrane protein T (OmpT), wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    OmpT (ompT E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMM2-hPMCA2x/b

    Plasmid

    #47582

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2x/b (ATP2B2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.2/V5 mmu-mir-153

    Plasmid

    #26330

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-153 (Mir153 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

40. pOmpW

    Plasmid

    #112121

    Purpose

    Bacterial expression of outer membrane protein W (OmpW), wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    OmpW (ompW E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAAV-OTp-hM3Dq-Myc

    Plasmid

    #184753

    Purpose

    To drive hM3D fused with Myc tag under the control of mouse oxytocin promoter

    Depositor

    Kazunari Miyamichi

    Article

    Inada et al Neuron. 2022 Apr 12. pii: S0896-6273(22)

    Insert

    hM3D (Gq)
    

    Use

    AAV

    Tags

    3xMyc

    Expression

    Mutation

    Promoter

    mouse Oxytocin promoter

    Available since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.2/V5 mmu-mir-138-1

    Plasmid

    #26328

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    mmu-mir-138-1 (Mir138-1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

43. pcDNA3.2/V5 hsa-mir-483

    Plasmid

    #26315

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-483 (MIR483 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

44. pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc)

    Plasmid

    #36879

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    tTA2
    tdT-3Myc
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCA-T-intron(Neo)-G(FLLFLNL)

    Plasmid

    #40021

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    T (ATG)
    GFP
    beta-globin intron
    Neo
    

    Use

    Tags

    Expression

    Mutation

    deleted nucleotides 1-274 and insertion of FRT, …

    Promoter

    CAG (chicken beta globin promoter and CMV enhance…

    Available since

    Oct. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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46. pGBW-m4046952

    Plasmid

    #145718

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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47. px330-RPL26 sgRNA2

    Plasmid

    #134642

    Purpose

    contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    RPL26 sgRNA2 (RPL26 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. px330-RPL26 sgRNA1

    Plasmid

    #134641

    Purpose

    contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    RPL26 sgRNA2 (RPL26 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pGBW-m4046938

    Plasmid

    #145599

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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50. pENTR1A-3xFlag-S242R-MEFV

    Plasmid

    #167020

    Purpose

    Gateway entry plasmid with 3xFlag-pyrin (S242R/PAAND variant)

    Depositor

    Thomas Henry

    Article

    Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

    Insert

    MEFV (MEFV Human)
    

    Use

    Gateway entry vector

    Tags

    3xFlag

    Expression

    Mutation

    serine 242 in arginine (S242R)

    Promoter

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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