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We narrowed to 37,163 results for: ELL

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Showing: 1841 - 1860 of 37163 results

  3. PB-TA-ERN

    Plasmid
    #80474
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Expression
    Mammalian
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80474-plasmid-map-sequence-id-151580

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  5. lentiCRISPR - EGFP sgRNA 2

    Plasmid
    #51761
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available Since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51761-plasmid-map-sequence-id-79377

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  11. pSCAR_Cas9-blast_GFP

    Plasmid
    #162074
    Purpose
    3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance
    Depositor
    Robert Manguso
    Article
    Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2
    Inserts
    Cas9-2a-blasticidin S deaminase
    EGFP
    Use
    CRISPR, Cre/Lox, and Lentiviral
    Expression
    Mammalian
    Mutation
    codon-optimized for expression in M. musculus
    Promoter
    EF1a and sv40
    Available Since
    Feb. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162074-plasmid-map-sequence-id-471574

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  16. pAd-M3cherry

    Plasmid
    #61041
    Purpose
    This plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.
    Depositor
    Qiao Zhou
    Article
    Li et al Nat Biotechnol. 2014 Dec;32(12):1223-30.
    Inserts
    Ngn3 (Neurog3 Mouse)
    pdx1 (Pdx1 Mouse)
    Mafa (Mafa Mouse)
    monomeric cherry
    Use
    Adenoviral
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    61041-plasmid-map-sequence-id-456476

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  18. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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Showing: 1841 - 1860 of 37163 results