341,370 results
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Plasmid#85452PurposeLentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.DepositorInsertU6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
UseCRISPR and LentiviralTagsNLSExpressionMammalianAvailable SinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q145
Plasmid#111731PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ145 (polyQ repeat)PromoterCMV and P10Available SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP
Plasmid#48885PurposePositive feedback plasmid from oscillatorDepositorInsertLuxI, sfGFP, and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialPromoterpLuxAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
eNme2C-CBE6b
Plasmid#215830PurposeExpress CBE6b (with eNme2-C variant) in mammalian cellsDepositorInsertCBE6b-eNme2C-2xUGI
ExpressionMammalianAvailable SinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER11 (miR-RUG)
Plasmid#44363PurposeInducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interestDepositorInsertPheS Gly294
UseLentiviralAvailable SinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B7
Plasmid#135509PurposeMammalian expression of myc-tagged HLA-B*07:02DepositorInsertHLA-B*07:02 (HLA-B Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
OMM-Flag-APEX2-SOPP3
Plasmid#229073PurposeExpresses APEX2-SOPP3 targeted to outer mitochondrial membraneDepositorInsertExpress APEX2-SOPP3 in outer mitochondrial membrane (APEX2 Human)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
HLA_A0101
Plasmid#165009PurposeExpression of HLA-A*01:01 cDNADepositorAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-p65
Plasmid#111190Purposefluorescent fusion proteinDepositorAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCB591
Plasmid#141096PurposeE. coli-Lactobacilli shuttle vector for recombineering template cloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIVM_02
Plasmid#204500PurposeExpression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)DepositorInsertamino acids 54-213 VHL (VHL Human)
Tags6xHis, TEV, and ThrombinExpressionBacterialMutation0Available SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-(empty)-TAG
Plasmid#138209PurposeTo facilitate restriction cloning of an RNA of interest with a TGT recognition element in the 3' regionDepositorTypeEmpty backboneUseT7 transcriptionTagsTAG sequenceExpressionMammalianPromoterCMV Promoter, T7 PromoterAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
M13cp-dg2 hp
Plasmid#218094PurposeHelper plasmid that allows phagemid production upon complementation with a phagemid expressing pIIDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA (AAV9)
Viral Prep#20299-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA (#20299). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent mCherry expression. These AAV preparations are suitable purity for injection into animals.DepositorTagsmCherry (Cre-dependent)Available SinceSept. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9 KRAB-T2A-GFP
Plasmid#67620PurposeCo-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFPDepositorInserthumanized dead Cas9 KRAB T2A GFP
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRLsinPGK_IRFP720_WPRE
Plasmid#91785PurposeConstitutive expression of IRFP720 using PGK promoter in a 3rd generation lentiviral backbone.DepositorInsertiRFP720
UseLentiviralExpressionMammalianPromoterPGKAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIVM_26
Plasmid#204501PurposeExpression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)DepositorExpressionBacterialMutation0PromoterAmpRAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT WT
Plasmid#84924Purposeluciferase reporter for TERT promoterDepositorAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-BFP-U6-sgRNA
Plasmid#196725PurposeSortable sgRNA expression, Chen tracrRNA PMID: 24360272DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
synIRES_RC25
Plasmid#189970PurposePart 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAsDepositorInsertsynIRES_RC25
UseCloningPromoterN/AAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
MRGPRX2-Tango
Plasmid#66440PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher002-oDi
Plasmid#124661PurposeExpresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coilDepositorInsertSpyCatcher002-oDi
TagsC-tag, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ras-LOCKR-S
Plasmid#216496PurposeFRET-based reporter for Ras activity, untargetedDepositorInsertRas-LOCKR-S
ExpressionMammalianAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.18 CMV-Luc
Plasmid#100984PurposeCMV luciferase reporter - firefly luciferase driven by CMV promoterDepositorInsertCMV
ExpressionMammalianAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1
Plasmid#193942Purposea fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.DepositorInsertheavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
UseRetroviralAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
NG-ABEmax
Plasmid#124163PurposeA-to-G base editorDepositorInsertTadA-TadA(evo)-Cas9-NG
ExpressionMammalianAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
ISceI-GR-RFP
Plasmid#17654DepositorTagsmRFPExpressionMammalianMutationC655G mutation in the glucocorticoid receptor (se…Available SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-mMLH1dn
Plasmid#174825PurposeMammalian expression of mouse MLH1dn (codon optimized)DepositorInsertmouse MLH1dn (codon optimized)
ExpressionMammalianMutationdeletion of residues 758-760PromoterEF1aAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC1-mitoRexYFP
Plasmid#60246PurposeMitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.DepositorInsertmitoRexYFP
TagsMitochondrial targeting signalExpressionMammalianPromoterCMVAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only