We narrowed to 11,027 results for: GFP expression plasmids

1. GW1-mito-mRuby2-roGFP1

   Plasmid

   #107219

   Purpose

   Mammalian expression plasmid to target the rmRuby1-roGFP1 sensor to the mitochondrial matrix.

   Depositor

   Mathew Tantama

   Article

   Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

   Insert

   mRuby2-roGFP1
   

   Use

   Tags

   4xCox8

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLenti-ZIKVA-GFP-puro

   Plasmid

   #140090

   Purpose

   Fluorescence activatable reporter of ZIKV NS2B-NS3 protease activity

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

   Insert

   ZIKVA-GFP
   

   Use

   Lentiviral; Low expression

   Tags

   6XHis tag and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-DENV2A-GFP-puro

   Plasmid

   #140089

   Purpose

   Fluorescence activatable reporter of DENV-2 NS2B-NS3 protease activity

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

   Insert

   DENV2A-GFP
   

   Use

   Lentiviral; Low expression

   Tags

   6XHis tag and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA-GFP-EBA175-Flag

   Plasmid

   #154970

   Purpose

   Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   PfEBA175
   

   Use

   Tags

   GFP and signal peptide (residues 1 to 32) from TG…

   Expression

   Mammalian

   Mutation

   insert codes for EBA175 residues 1284 to 1462

   Promoter

   CMV

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPLN-hFluc-DM-eGFP-KDEL

   Plasmid

   #177728

   Purpose

   DM Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFlucDM
   

   Use

   Tags

   KDEL, Prolactin Signal Sequence, and eGFP

   Expression

   Bacterial and Mammalian

   Mutation

   R188Q, R261Q (Destabilized Mutant)

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFUGW-GFP-2-cut reporter

   Plasmid

   #131669

   Purpose

   Lentiviral plasmid that expresses a GFP-based reporter for monitoring CRISPR based precise end-joining.

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   GFP-2-cut reporter
   

   Use

   Lentiviral

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-APOBEC1-YTH-EGFP

   Plasmid

   #209322

   Purpose

   AAV packaging vector containing a APOBEC1-YTH expression, a P2A-EGFP expression cassette,.

   Depositor

   Magdalena Koziol

   Article

   Feng et al Genome Res. 2024 Jul 23;34(6):822-836. d

   Insert

   APOBEC1-YTH-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FUGW-UBC-EGFP-2A-dnVAMP2

   Plasmid

   #185723

   Purpose

   Lentiviral expression of GFP and dominant-negative VAMP2 from UbC promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   VAMP-2 (VAMP2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   deleted AA 97-116

   Promoter

   hUbC

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPLN-hFluc-WT-eGFP-KDEL

   Plasmid

   #177727

   Purpose

   Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention and fused to eGFP at the C-terminus

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   hFluc
   

   Use

   Tags

   KDEL, Prolactin Signal Sequence, and eGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. VP2C-GFP-MIOX only

    Plasmid

    #166684

    Purpose

    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    VP2C-GFP-MIOX
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJFRC81-3xVAS3-IVS-Syn21-GFP-P10

    Plasmid

    #104614

    Purpose

    Expresses GFP upon binding of TALE3 to VAS3

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    3xVAS3
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJFRC81-3xVAS1-IVS-Syn21-GFP-P10

    Plasmid

    #104613

    Purpose

    Expresses GFP upon binding of TALE1 to VAS1

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    3xVAS1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJFRC81-3xVAS4-IVS-Syn21-GFP-P10

    Plasmid

    #104615

    Purpose

    Expresses GFP upon binding of TALE4 to VAS4

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    3xVAS4
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    hsp70

    Available since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOTTC1553 - pAAV EF1a EGFP-KASH

    Plasmid

    #131682

    Purpose

    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP

    Depositor

    Christopher Richie

    Article

    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

    Insert

    EGFP-KASH
    

    Use

    AAV

    Tags

    KASH

    Expression

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAM-AAV-mSncg-EGFP

    Plasmid

    #153163

    Purpose

    Mouse gamma-synuclein (mSncg) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mSncg

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAM-AAV-mCamk2a-EGFP

    Plasmid

    #153197

    Purpose

    Mouse Camk2a (mCamk2a) promoter-mediates gene expression in retinal neurons with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAM-AAV-hSyn1-EGFP

    Plasmid

    #153205

    Purpose

    Human synapsin-1 (hSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAM-AAV-mSyn1-EGFP

    Plasmid

    #153187

    Purpose

    Mouse synapsin-1 (mSyn1) promoter-mediates gene expression in retinal neurons with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAM-AAV-CAG-EGFP

    Plasmid

    #153186

    Purpose

    CAG promoter-mediates gene expression in retinal neurons with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAM-AAV-hSncg-EGFP

    Plasmid

    #153198

    Purpose

    Human gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAM-AAV-hIsl2-EGFP

    Plasmid

    #153206

    Purpose

    Human Isl2 (hIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-mDlx-GFP-Fishell-1

    Plasmid

    #83900

    Purpose

    GFP expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer element

    Depositor

    Gordon Fishell

    Article

    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    mDlx

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. VI_pAAV-ProA7-GFP-WPRE

    Plasmid

    #125891

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProA7

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. VII_pAAV-ProB8-GFP-WPRE

    Plasmid

    #125928

    Purpose

    Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

    Depositor

    Botond Roska

    Article

    Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    ProB8

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-CaMKII-Jaws-KGC-GFP-ER2

    Plasmid

    #65015

    Purpose

    AAV-mediated expression of Jaws under the CaMKII promoter

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    CaMKII

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-hsyn-Jaws-KGC-GFP-ER2

    Plasmid

    #65014

    Purpose

    AAV-mediated expression of Jaws under the human synapsin promoter

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Has Service

    AAV Retrograde, AAV5, and AAV8

    Insert

    Jaws-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    human synapsin

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FUW-ubiquitin-EphB2-SV40-GFP (B2G)

    Plasmid

    #65442

    Purpose

    Expression of mouse EPHB2 and GFP in mammalian cells

    Depositor

    Eduard Batlle

    Article

    Cortina et al Nat Genet. 2007 Nov;39(11):1376-83. Epub

    Insert

    EPHB2 (Ephb2 Mouse)
    

    Use

    Lentiviral

    Tags

    SV40-EGFP

    Expression

    Mammalian

    Mutation

    See depositor comments below.

    Promoter

    UBQ

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOTTC710 - pAAV EF1a DIO Mem-AcGFP

    Plasmid

    #75081

    Purpose

    An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

    Insert

    Mem-AcGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Palmitylation site

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MIGR1_FLAG_TAL1-long_GFP

    Plasmid

    #210638

    Purpose

    Overexpression of TAL1-long

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1-short (TAL1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MIGR1_FLAG_TAL1-short_GFP

    Plasmid

    #210637

    Purpose

    Overexpression of TAL1-short

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1-short (TAL1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40]

    Plasmid

    #124069

    Purpose

    CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony and Dm-yellow for linearization; (Transformation plasmid; Black eye marker)

    Depositor

    Gregor Bucher

    Insert

    eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Insect

    Mutation

    Promoter

    bhsp68

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-SynI-CreOn-FlpOff-EGFP

    Plasmid

    #176274

    Purpose

    Viral vector for expression of EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin I

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-CAG-miniGFP2-P2A-FusionRed

    Plasmid

    #188734

    Purpose

    Express miniGFP2 and FusionRed in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP2
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GFP-CaM-NanoLuc-15aa-uTEVp

    Plasmid

    #163034

    Purpose

    FLiCRE protease with luciferin-gating for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    GFP-CaM-NanoLuc-15aa-uTEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAAV-CAG-miniGFP1-P2A-FusionRed

    Plasmid

    #188732

    Purpose

    Express miniGFP1 and FusionRed in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP1
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRSFDUET-1_VNp-anti-GFP mAb

    Plasmid

    #214955

    Purpose

    Bacterial expression of anti-GFP mAb light and heavy chain VNp fusions.

    Depositor

    Dan Mulvihill

    Article

    Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

    Inserts

    VNp-anti-GFP mAb heavy chain
    VNp-anti-GFP mAb light chain
    

    Use

    Tags

    VNp6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-Syn-ChR2(H134R)-GFP

    Plasmid

    #58880

    Purpose

    AAV expression of ChR2(H134R)-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

    Has Service

    AAV Retrograde and AAV8

    Insert

    ChR2(H134R)-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    Syn

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLV CDHblast Rev(V16D)-EGFP-V5

    Plasmid

    #175165

    Purpose

    Lentiviral expression of Rev(V16D)-EGFP-V5

    Depositor

    Larry Gerace

    Article

    Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

    Insert

    rev
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    V16D

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCIG3 (pCMV-IRES-GFP version 3)

    Plasmid

    #78264

    Purpose

    Lentiviral empty cloning vector containing IRES and GFP

    Depositor

    Felicia Goodrum

    Article

    Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, lentiviral LTR

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-CASI-Cas9C-P2A-turboGFP

    Plasmid

    #80942

    Purpose

    Expresses Cas9C in mammalian cells; co-expresses turboGFP.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCXLE-dCas9VPH-T2A-GFP-shP53

    Plasmid

    #102895

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9VPH-T2A-GFP-shP53
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. LZF1826 pAAV_hSyn-DiO-SomArchon-EGFP-P2A-stGtACR2

    Plasmid

    #135412

    Purpose

    simultaneous optical recording and manipulation of electrical activity in neurons

    Depositor

    Adam Cohen

    Article

    Fan et al Cell. 2020 Jan 23. pii: S0092-8674(20)30

    Insert

    SomArchon-EGFP-P2A-stGtACR2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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43. pAAV-EF1a-Flex-PAK1-AID-ires-EGFP

    Plasmid

    #83489

    Purpose

    cre-dependent PAK1-AID under EF1a promoter

    Depositor

    Dong Kong

    Article

    Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

    Insert

    PAK1-AID-ires-EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCMV-2E2-HA-mEGFP

    Plasmid

    #129595

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pCMV-15F11-HA-mEGFP

    Plasmid

    #129590

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pEGFP-C3-PLK4-3xFLAG

    Plasmid

    #69837

    Purpose

    This plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Use

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

    Plasmid

    #172067

    Purpose

    Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP11x7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBS-KS-attB1-2-PT-SA-SD-1-GFP11

    Plasmid

    #172068

    Purpose

    Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP11x7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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49. AAV-SYN_mEGFP-CREB

    Plasmid

    #137006

    Purpose

    AAV backbone, mEGFP-CREB donor for G-CREB

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

    Insert

    CREB1 (Creb1 Rat)
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pLenti HsATP10B - EmGFP

    Plasmid

    #204474

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B WT fused with EmGFP

    Depositor

    Veerle Baekelandt

    Article

    Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

    Inserts

    ATP10B (ATP10B Human)
    EmGFP
    

    Use

    Lentiviral

    Tags

    EmGFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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