Showing: 21 - 40 of 92 results
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Plasmid#112917PurposeFluorescence microscopyDepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPS7 promoter and Ps7AvailabilityAcademic Institutions and Nonprofits only -
pMLBAD-eGFP
Plasmid#112918PurposeFluorescence microscopyDepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterPbadAvailabilityAcademic Institutions and Nonprofits only -
pTNS3
Plasmid#63127PurposeTn7 transposase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterP1 and PtacAvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21a-BpeR
Plasmid#51159DepositorInsertBpeR
UseSynthetic BiologyTags6x-hisExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI
Plasmid#32061PurposeConstruction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonucleaseDepositorInsertI-SceI nuclease
UseTagsExpressionBacterialMutationPromoterPdhfrAvailabilityAcademic Institutions and Nonprofits only -
pDA12
Plasmid#113663PurposeCloning vector for bacterial expression with Pdhfr promoterDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPdhfrAvailabilityAcademic Institutions and Nonprofits only -
pSC201plus
Plasmid#164227PurposeIntegrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expressionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterRhamnose-inducibleAvailabilityAcademic Institutions and Nonprofits only -
pAH25-SceI
Plasmid#129389PurposeDerived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.DepositorInsertChloramphenicol acetyltransferase
UseTagsExpressionBacterialMutationN/APromoterCAT promoterAvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rha
Plasmid#129390PurposeDerived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.DepositorInsertRhamnose-inducible promoter
UseTagsExpressionBacterialMutationN/APromoterPrhaBADAvailabilityAcademic Institutions and Nonprofits only -
pSCB2-sgRNA
Plasmid#129463PurposeTemplate plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).DepositorInsertpgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-guideless
Plasmid#129464PurposeDerived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.DepositorInsertGuideless gRNA backbone
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)AvailabilityAcademic Institutions and Nonprofits only -
pDAI-SceI-SacB
Plasmid#113635PurposeBroad host range replicative plasmid expressing the I-SceI homing endonuclease and the counterselectable marker SacBDepositorInsertsacB
UseTagsExpressionBacterialMutationPlease see Depositor CommentsPromotersacB promoterAvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2plus
Plasmid#164226PurposeBroad-range bacterial expression vector with modified rhamnose-inducible promoter. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expression.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterRhamnose-inducibleAvailabilityAcademic Institutions and Nonprofits only -
pgRNA-non-target
Plasmid#129465PurposeDerived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.DepositorInsertNon-target/random gRNA
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 (SpeI)AvailabilityAcademic Institutions and Nonprofits only -
pGPΩTp
Plasmid#130660PurposeInsertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.DepositorInsertΩ trimethoprim cassette
UseTagsExpressionBacterialMutationAddition of Ω sites next to trimethoprim cassette…Promoterdhfr promoterAvailabilityAcademic Institutions and Nonprofits only -
pCM184-ParB2::eGFP
Plasmid#87252PurposeExpresses ParB2-EGFP in yeast. Also known as pOR2-GFPDepositorInsertParB2
UseTagsEGFPExpressionYeastMutationPromoterpBAD/tetOAvailabilityAcademic Institutions and Nonprofits only -
pCM189-ParB1::mCherry
Plasmid#87253PurposeExpresses ParB1-mCherry in yeast. Also known as pOR1-mchDepositorInsertParB1
UseTagsmCherryExpressionYeastMutationPromoterpBAD/tetOAvailabilityAcademic Institutions and Nonprofits only -
pVRa37_2513
Plasmid#49696DepositorInsertECF37_2513
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailabilityAcademic Institutions and Nonprofits only -
pVRc37_2513
Plasmid#49751DepositorInsertAS37_2513
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rhadCas9-native
Plasmid#129392PurposeDerived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.DepositorInsertS. pyogenes dCas9
UseCRISPRTagsExpressionBacterialMutationAspartate 10 to Alanine, Histidine 840 to AlaninePromoterPrhaBADAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 92 results