Addgene

We narrowed to 392 results for: pth

Showing: 21 - 40 of 392 results

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pTHSSd_40

Plasmid

#59963

Purpose

High core fragment expression without SynZIP

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

core fragment lacking SynZIP

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Availability

Academic Institutions and Nonprofits only

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pTHSSd_41

Plasmid

#59964

Purpose

High full length T7 RNAP* expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

T7* RNAP

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Availability

Academic Institutions and Nonprofits only

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pTHSSd_42

Plasmid

#59965

Purpose

beta core fragment expression

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

beta core fragment

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

PJ23105

Availability

Academic Institutions and Nonprofits only

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pTHSSd_44

Plasmid

#59966

Purpose

constitutive expression of T7 sigma fragment

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

T7 sigma fragment

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Pcon sigma

Availability

Academic Institutions and Nonprofits only

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pTHE

Plasmid

#12512

Depositor

Tong-Chuan He

Article

Jiang et al J Biol Chem. 2001 Nov 30. 276(48):45168-

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pThuS

Plasmid

#171674

Purpose

ThuS expression in E. coli

Depositor

Wilfred van der Donk

Article

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

Glycosyltransferase ThuS

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pThuAsol

Plasmid

#171675

Purpose

pThuAsol expression in E. coli

Depositor

Wilfred van der Donk

Article

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

SUMO-TEV-ThuAsol

Use

Tags

His-tag and SUMO-tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pThermoCas9_ctrl

Plasmid

#100981

Purpose

Expresses ThermoCas9 and its sgRNA module

Depositor

John van der Oost

Article

Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

Inserts

Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

B. coagulans DSM 1 pta promoter and B. smithii xy…

Availability

Academic Institutions and Nonprofits only

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pThermoCas9i_ctrl

Plasmid

#100982

Purpose

Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module

Depositor

John van der Oost

Article

Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

Inserts

Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module

Use

Tags

None

Expression

Bacterial

Mutation

changed aspartate 8 to alanine and histidine 582 …

Promoter

B. coagulans DSM 1 pta promoter and B. smithii xy…

Availability

Academic Institutions and Nonprofits only

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pThermeleon

Plasmid

#86120

Purpose

Bandpass temperature-gated expression of mWasabi and mCherry reporters

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi
TcI38
mCherry
cI

Use

Tags

6xHis

Expression

Bacterial

Mutation

A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…

Promoter

pR/pL, pR/pL, pLacI, and pTlpA

Availability

Academic Institutions and Nonprofits only

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BB1_12_pTHI11

Plasmid

#98512

Purpose

BB1 with insert: promoter PTHI11

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

THI11 promoter

Use

Tags

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Promoter

Availability

Academic Institutions and Nonprofits only

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pThom77.3

Plasmid

#101636

Purpose

Encodes the fluorescent protein amFP486/K68M for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

amFP486/K68M

Use

Tags

6XHis

Expression

Bacterial

Mutation

K68M

Promoter

Availability

Academic Institutions and Nonprofits only

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pThom77.6

Plasmid

#101637

Purpose

Encodes the fluorescent protein amFP486/K68M under a CMV promoter.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

amFP486/K68M

Use

Tags

Expression

Mammalian

Mutation

K68M

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pThom77.2

Plasmid

#101646

Purpose

Encodes the fluorescent protein amFP486 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

amFP486

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MAC_C_PTH2

Plasmid

#111674

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

PTH2 (PTRH2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTH07

Plasmid

#70063

Purpose

artificial transcription template "distal pause"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - repeat cassette - E. coli rpoB with inserted pause - lambda TR'

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pTH09

Plasmid

#70064

Purpose

artificial transcription template "terminator insertion"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - lambda TR2 - repeat cassette - E. coli rpoB - lambda TR'

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only
pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

Plasmid

#129105

Purpose

Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production

Depositor

Eleftherios Terry Papoutsakis

Article

Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

Insert

BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

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pTH1

Plasmid

#84451

Purpose

shuttle plasmid for sarA P1-dsRED expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

DsRed.T3(DNT)

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTH2

Plasmid

#84452

Purpose

shuttle plasmid for sarA P1-CFP expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

CFP Cerulean

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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