422 results

1. EcM2.1

   Bacterial Strain

   #64052

   Purpose

   Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

   Depositor

   George Church

   Article

   Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

   Bacterial Resistance

   Ampicillin

   Available since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

2. eScaf

   Bacterial Strain

   #220921

   Purpose

   cssDNA production strain

   Depositor

   Shawn Douglas

   Article

   Shen et al Nucleic Acids Res. 2024 Apr 24;52(7):409

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

3. bMS.346

   Bacterial Strain

   #220588

   Purpose

   Increase editing efficiency of retron and oligo recombineering

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Bacterial Resistance

   None

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

4. JM101

   Bacterial Strain

   #50349

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Messing et al Recombinant DNA Technical Bulletin, 1979

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. KJ740(DE3)

   Bacterial Strain

   #179486

   Purpose

   KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression

   Depositor

   Debra Hansen

   Article

   Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

   Bacterial Resistance

   Tetracycline (chromosome, tn10) (10 μg/ml); streptomycin/spectinomycin (chromosome) (50 μg/ml)

   Species

   Escherichia coli

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

6. EC1000

   Bacterial Strain

   #71852

   Purpose

   RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

   Depositor

   Todd Klaenhammer, Jan Kok

   Article

   Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

   Bacterial Resistance

   Kanamycin

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

7. LC-E75

   Bacterial Strain

   #115925

   Purpose

   Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Bacterial Resistance

   None

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

8. C321.∆A T7RNAP ompT- rne-

   Bacterial Strain

   #182777

   Purpose

   Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

   Depositor

   Michael Jewett

   Article

   Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

   Bacterial Resistance

   Spectinomycin

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

9. MG1655 ΔendA ΔrecA

   Bacterial Strain

   #37853

   Depositor

   Kristala Prather

   Article

   Leonard et al Proc Natl Acad Sci U S A. 2010 Aug 3;107

   Bacterial Resistance

   None

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

10. V. natriegens NC1

    Bacterial Strain

    #215355

    Purpose

    Vibrio natriegens with genomically integrated natural competence regulator, TfoX, with chloramphenicol resistance. For zero-capital molecular biology applications.

    Depositor

    Buz Barstow

    Article

    Specht et al PNAS Nexus. 2024 Feb 13;3(2):pgad444. do

    Bacterial Resistance

    None

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

11. ATMW-BL21

    Bacterial Strain

    #218769

    Purpose

    E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

    Depositor

    Abhishek Chatterjee

    Article

    Ficaretta et al J Mol Biol. 2022 Apr 30;434(8):167304. d

    Bacterial Resistance

    Spectinomycin (50 ug/ml), gentamycin (10 ug/ml)

    Species

    Escherichia coli

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

12. sAJM.1504

    Bacterial Strain

    #108251

    Purpose

    Marionette-Clo. Marionette cluster inserted in the direction of leading strand replication, between 3,957,594 and 3,959,211 in E. coli DH10B

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Bacterial Resistance

    Chloramphenicol

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

13. EcNR2 Strain

    Bacterial Strain

    #26931

    Depositor

    George Church

    Article

    Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

    Bacterial Resistance

    Chloramphenicol and ampicillin

    Available since

    Jan. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pRK24

    Bacterial Strain

    #51950

    Purpose

    broad-host range natural conjugative plasmid

    Depositor

    Farren Isaacs

    Article

    Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

    Bacterial Resistance

    Tetracycline

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

15. BL21ΔABCF

    Bacterial Strain

    #102270

    Purpose

    ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins

    Depositor

    Jack Leo

    Article

    Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

    Bacterial Resistance

    Tetracycline

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

16. Syn61Δ3(ev5) ΔrecA (ev1)

    Bacterial Strain

    #189857

    Purpose

    Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

    Depositor

    George Church

    Article

    Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

    Bacterial Resistance

    Streptomycin

    Species

    Synthetic

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

17. DH5alpha::DddI

    Bacterial Strain

    #179631

    Purpose

    For expression of toxic DddA

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Bacterial Resistance

    Gentamicin

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

18. rEcoli XpS

    Bacterial Strain

    #192872

    Purpose

    MG1655-C321 -- All 321 UAG codons changed to UAA

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

    Bacterial Resistance

    Bleocin (zeocin)

    Species

    Escherichia coli

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

19. E. coli MEV20

    Bacterial Strain

    #197113

    Purpose

    E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Bacterial Resistance

    None

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

20. C321.∆A.opt

    Bacterial Strain

    #87359

    Purpose

    This is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.

    Depositor

    George Church

    Article

    Kuznetsov et al Genome Biol. 2017 May 25;18(1):100. doi:

    Bacterial Resistance

    Gentamicin

    Species

    Synthetic

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only