159,428 results

1. anti-FLAG M2 heavy chain

   Plasmid

   #175359

   Purpose

   Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

   Depositor

   Joost Snijder

   Article

   Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

   Insert

   anti-FLAG M2 heavy chain
   

   Use

   Tags

   His8 tag and N-terminal secretion signal peptide …

   Expression

   Mammalian

   Mutation

   L51I (see paper)

   Promoter

   CMV

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-Clover-HIF-1alpha Wildtype

   Plasmid

   #163365

   Purpose

   Express HA- and Clover-tagged HIF-1alpha wildtype plasmid

   Depositor

   Violaine Sée

   Insert

   Hypoxia Inducible Factor 1 alpha (HIF1A Human)
   

   Use

   Tags

   HA- and Clover-tagged

   Expression

   Mammalian

   Mutation

   Wildtype

   Promoter

   CMV Promoter

   Available since

   Jan. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL1765 (pSPIN-R, R*MmeI)

   Plasmid

   #160734

   Purpose

   Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-Sec23A

   Plasmid

   #66609

   Purpose

   GFP fusion of Sec23A

   Depositor

   David Stephens

   Article

   Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

   Insert

   Sec23A (SEC23A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFB2G

   Plasmid

   #195290

   Purpose

   Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

   Depositor

   Scott Lowe

   Article

   Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

   Insert

   HSV-TK 2A mTagBFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMitoLOC

   Plasmid

   #58980

   Purpose

   Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

   Depositor

   Markus Ralser

   Article

   Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

   Inserts

   mCherry
   GFP
   

   Use

   Tags

   preCOX4 and preSU9

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 and MET17

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

   Plasmid

   #98927

   Purpose

   Cre dependent AAV expression of GFP spaghetti monster myc construct

   Depositor

   Loren Looger

   Article

   Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

   Has Service

   AAV1

   Insert

   GFP-smFP-myc
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpyCatcher003-mi3

   Plasmid

   #159995

   Purpose

   Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyCatcher003-mi3
   

   Use

   Tags

   C-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. HS-GFP-PACT

   Plasmid

   #105954

   Purpose

   heat shock inducible transgenesis, PACT is a centriol marker

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   PACT (AKAP9 Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM2991

    Plasmid

    #40248

    Depositor

    Susan Golden

    Article

    Ivleva et al EMBO J. 2005 Mar 23;24(6):1202-10. Epub

    Insert

    One Step Cloneing Vector for Overexpression
    

    Use

    Cyanobacteria cloning vector

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. AiP12315 - pAAV-AiE0464m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2315)

    Plasmid

    #223981

    Purpose

    AiE0464m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. phCMV-GALV-MTR

    Plasmid

    #163612

    Purpose

    Retro and Lentiviral transduction of primary human germinal center B cells

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Insert

    GaLV MTR envelope
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG G-CEPIA1er

    Plasmid

    #105012

    Purpose

    ER calcium sensor

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

    Insert

    G-CEPIA1er
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. E228Q Vps4A-HA

    Plasmid

    #200087

    Purpose

    3x HA-VPS4A with E228Q dominant negative mutation in Dox-inducible pInducer20 vector

    Depositor

    Jason MacGurn

    Article

    Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

    Insert

    VPS4A (VPS4A Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    E228Q

    Promoter

    CMV

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 Flag-humanTRAF2 (1087)

    Plasmid

    #66931

    Purpose

    Mammalian Expression of TRAF2

    Depositor

    Michael Karin

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid

    #48140

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO-RB1-shRNA19

    Plasmid

    #25640

    Purpose

    3rd generation lentiviral vector

    Depositor

    Todd Waldman

    Article

    Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

    Insert

    RB1-shRNA 19 (RB1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCW01

    Plasmid

    #72300

    Purpose

    Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Type

    Empty backbone

    Use

    Derivative of puc19

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PLKO-mcherry-luc-puro-TRPM1 cDNA

    Plasmid

    #29782

    Depositor

    Carl Novina

    Article

    Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

    Insert

    TRPM1 (TRPM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHCMV-VSVgSS-pAG-TM

    Plasmid

    #207318

    Purpose

    Functional incorporation of protein AG (pAG) fusion protein into envelope of virions

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    protein AG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFP-HP1alpha

    Plasmid

    #179701

    Purpose

    visualization of centromere heterochromatin

    Depositor

    Kenji Sugimoto

    Article

    Yamada et al J Biochem. 1999 Apr . 125(4):832-7.

    Insert

    heterochromatin protein alpha (CBX5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. lentiBE3-SpRY-Blast

    Plasmid

    #199303

    Purpose

    Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    FNLS-BE3-SpRY
    

    Use

    CRISPR and Lentiviral

    Tags

    2A tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pPIPL12-FnCas12a

    Plasmid

    #199563

    Purpose

    Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

    Depositor

    Philippe Lambin

    Article

    Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

    Insert

    Fncas12a
    

    Use

    CRISPR and Synthetic Biology; Tetracycline induci…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    COtetR-PIPL12

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pPfetFn-Target_v2

    Plasmid

    #199564

    Purpose

    gRNA expression for FnCas12a in E. coli and clostridia

    Depositor

    Philippe Lambin

    Article

    Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

    Insert

    SacB
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pthl_fU

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pmCherry-HP1alpha

    Plasmid

    #179703

    Purpose

    visualization of centromere heterochromatin

    Depositor

    Kenji Sugimoto

    Article

    Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

    Insert

    heterochromatin protein alpha (CBX5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pcDNA3-hPar2

    Plasmid

    #53228

    Purpose

    mammalian expression of human Par2

    Depositor

    Shaun Coughlin

    Article

    Bohm et al Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3)

    Insert

    Par2 (F2RL1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LentiCRISPRv2 RFP670

    Plasmid

    #187646

    Purpose

    lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site

    Depositor

    Martin Carroll

    Insert

    RFP 670
    

    Use

    Lentiviral

    Tags

    RFP670 and sgRNA cloning site

    Expression

    Mutation

    Promoter

    EFS (P2A)

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pRSET his-eGFP

    Plasmid

    #113551

    Purpose

    For bacterial expression and purification of eGFP

    Depositor

    Jeanne Stachowiak

    Article

    Busch et al Nat Commun. 2015 Jul 24;6:7875. doi: 10.

    Insert

    his-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A206K

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pT077

    Plasmid

    #137879

    Purpose

    Donor vector for integration into the human AAVS1 safe harbor locus of Doxicycline-inducible KRAB-dCas9-IRES-EGFP

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TRE3G-KRAB-dCas9-IRES-EGFP-CAG-TetON-NeoR-SA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBiFC-VC155

    Plasmid

    #22011

    Purpose

    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    VC155
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Changed Alanine 206 to Lysine

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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