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pLexM-SLC3A2

Plasmid

#197994

Purpose

Expresses SLC3A2 as an N-terminal His tagged construct in mammalian cells

Depositor

Simon Newstead

Article

Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

Insert

SLC3A2 (SLC3A2 Human)

Tags

His tag

Expression

Mammalian

Mutation

Please see depositor comments below

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-RIG-I

Plasmid

#240814

Purpose

Expresses myc-tagged RIG-I in mammalian cells

Depositor

AJ te Velthuis

Insert

RIG-I (RIGI Human)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF2908

Plasmid

#143663

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NFIC (NFIC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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Active Wnt5A-V5

Plasmid

#43813

Depositor

Xi He

Article

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

WNT5A (WNT5A Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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PB-CAG-CasRX-P2A-EGFP-BGH PolyA

Plasmid

#154004

Purpose

Express CasRx

Depositor

Hui Yang

Article

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

CasRx

Use

CRISPR

Promoter

CAG

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGM1190

Plasmid

#69994

Purpose

OriT containing mobilizable vector for thiostrepton induced gene expression in Streptomyces

Depositor

Günther Muth

Article

Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

Inserts

aac
tsr
rep
sso
TipA promoter
dso

Expression

Bacterial

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6b-V106W

Plasmid

#215825

Purpose

Express CBE6b V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6b V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti HRE-Luc pGK Hygro

Plasmid

#118706

Purpose

reporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferase

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

HRE (HIF-responsive promoter (3xHRE)-luc

Use

Lentiviral

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Hygro-mTagBFP2

Plasmid

#99374

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Inserts

S. pyogenes sgRNA cassette
Hygro-P2A-mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a and hU6

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-AXL

Plasmid

#116714

Purpose

Lentiviral expression of AXL

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AXL (AXL Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGoldenGate-SE9

Plasmid

#47677

Purpose

Binary vector for Arabidopsis transformation with BsaI Golden Gate sites and Kanamycin selection.

Depositor

Jose Dinneny

Article

Emami et al Front Plant Sci. 2013 Sep 2;4:339. doi:

Type

Empty backbone

Use

Arabidopsis transformation

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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mEGFP-C1

Plasmid

#54759

Purpose

Localization: C1 Cloning Vector, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

mEGFP

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCfB2793

Plasmid

#64043

Purpose

pCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter and none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLCN DSB Repair Reporter (DRR)

Plasmid

#98895

Purpose

DSB Repair Reporter (DRR). Expresses GFP after cut of two inverted ISce1 sites and repair by NHEJ.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

Neomycin-T2A-ISce1-ISce1-eGFP

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-EphrinB3-Flag

Plasmid

#155012

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

EphrinB3 (EPHB3 Human)

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Mito-LOV-Turbo-V5

Plasmid

#199666

Purpose

expresses LOV-Turbo in the mammalian mitochondria, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

COX4I1 mito targeting sequence and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pFuse-STOP

Plasmid

#217677

Purpose

Empty vector control plasmid containing a stop codon prior to the Fc encoding region

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Type

Empty backbone

Expression

Mammalian

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-NDP52

Plasmid

#135296

Purpose

stably express GFP-tagged NDP52 for CID induced mitophagy

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

NDP52 (CALCOCO2 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-GRAM-W

Plasmid

#211701

Purpose

Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cells

Depositor

Yasunori Saheki

Article

Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

Insert

GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Glycine 187 to Tryptophan

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRDB_161

Plasmid

#216091

Purpose

Cas9 [Sp] CRISPRi targeting CD81, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD81 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6

Plasmid

#177849

Purpose

Bacterial Expression of PNR

Depositor

Karsten Melcher

Article

Tan et al PLoS One. 2013 Sep 12;8(9):e74359. doi:

Insert

photoreceptor cell-specific nuclear receptor (NR2E3 Human)

Tags

6xHis and MBP

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ClpX

Plasmid

#234733

Purpose

Human-codon optimized E. coli ClpX in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

ClpX

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-MYC-IRES-luc

Plasmid

#129775

Purpose

Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

Depositor

Amaia Lujambio

Article

Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

Insert

human MYC (MYC Human)

Tags

luciferase

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_CRF1.0

Plasmid

#208655

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3520

Plasmid

#144996

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TCF4 (TCF4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp6-mNeongreen

Plasmid

#182388

Purpose

Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCW empty vector

Plasmid

#184708

Purpose

Lentiviral empty vector for doxycycline-inducible expression

Depositor

Alessia Ciarrocchi, Gloria Manzotti

Article

Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB3

Plasmid

#116735

Purpose

Lentiviral expression of ERBB3

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF2537

Plasmid

#143190

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SOX7 (SOX7 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C58

Plasmid

#171428

Purpose

Expression of FKBP-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

FKBP-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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