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27,467 results

Showing: 2061 - 2080 of 27467 results

  12. pCMV-PE6a

    Plasmid
    #207851
    Purpose
    Mammalian expression of PE6a prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6a
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    207851-plasmid-map-sequence-id-450977

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  14. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid
    #139988
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    139988-plasmid-map-sequence-id-275338

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  16. pLifeAct-HyPer7

    Plasmid
    #136464
    Purpose
    Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging
    Depositor
    Vsevolod Belousov
    Publication
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyPer7
    Tags
    LifeAct
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    136464-plasmid-map-sequence-id-271538

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  17. pDisplay-GACh2.0

    Plasmid
    #106073
    Purpose
    express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells
    Depositor
    Yulong Li
    Publication
    Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based ACh sensor (GACh) 2.0
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106073-plasmid-map-sequence-id-260902

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  18. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid
    #104567
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    Use
    CRISPR
    Tags
    NLS-3xHA-VPR
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104567-plasmid-map-sequence-id-201429

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  19. Ezh2[FL]-dCas9

    Plasmid
    #100086
    Purpose
    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    14056 (Ezh2 Mouse)
    Use
    CRISPR
    Tags
    3XFLag-NLS-Ezh2[FL]-dCas9-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100086-plasmid-map-sequence-id-197550

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  20. pCMV-pro-Il1b-C-Flag

    Plasmid
    #75131
    Purpose
    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells
    Depositor
    Bruce Beutler
    Publication
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    interleukin 1 beta (Il1b Mouse)
    Tags
    3XFLAG
    Expression
    Mammalian
    Mutation
    (please see depositor comments below)
    Promoter
    CMV
    Available since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75131-plasmid-map-sequence-id-192693

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Showing: 2061 - 2080 of 27467 results