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27,466 results

Showing: 3881 - 3900 of 27466 results

  3. G9A[SET]-dCas9

    Plasmid
    #100089
    Purpose
    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G9A (EHMT2 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-G9A[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    G9A[SET] includes aa 829-1209
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100089-plasmid-map-sequence-id-197561

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  5. mEGFP-PI35P2

    Plasmid
    #92419
    Purpose
    Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.
    Depositor
    Geert van den Bogaart
    Publication
    Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MCOLN1 (Mcoln1 Mouse)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Synthetic gene, codon optimized for human.
    Promoter
    CMV
    Available since
    July 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92419-plasmid-map-sequence-id-182509

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  7. pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

    Plasmid
    #69138
    Purpose
    For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo
    Depositor
    Takuji Iwasato
    Publication
    Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
    Tags
    none
    Expression
    Mammalian
    Available since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69138-plasmid-map-sequence-id-165113

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  8. pNTI194

    Plasmid
    #84266
    Purpose
    Expresses Avi-tagged RPL1 and humanized birA
    Depositor
    Nicholas Ingolia
    Publication
    Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    RPL1 (RPL10A (*see below), Human)
    humanized birA
    Tags
    Avi tag, Flag, Gly/Ser linker, and TEV site
    Expression
    Mammalian
    Mutation
    R33A, A34E (*see below)
    Promoter
    CMV and P2A peptide
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84266-plasmid-map-sequence-id-160813

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  11. pMito-miRFP703

    Plasmid
    #80000
    Purpose
    Mitochondria labeling with near-infrared fluorescent protein miRFP703
    Depositor
    Vladislav Verkhusha
    Publication
    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cytochrome C oxidase subunit VIII (COX8A Human)
    Tags
    miRFP703
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80000-plasmid-map-sequence-id-150089

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  12. pGCaMP6m-N3-TPC2

    Plasmid
    #80147
    Purpose
    GCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real time
    Depositor
    Santiago Di Pietro
    Publication
    Ambrosio et al Mol Biol Cell. 2015 Sep 15;26(18):3263-7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TPCN2 (TPCN2 Human)
    Tags
    GCaMP6m
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80147-plasmid-map-sequence-id-150677

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  13. CAG sHRPb-NLG

    Plasmid
    #73144
    Purpose
    Small fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sHRPb-NLG (Nlgn1 Mouse, Synthetic)
    Tags
    V5 tag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73144-plasmid-map-sequence-id-134514

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  16. PXL

    Plasmid
    #75349
    Purpose
    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.
    Depositor
    Bryan Luikart
    Publication
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75349-plasmid-map-sequence-id-266810

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  18. Cx43-msfGFP

    Plasmid
    #69007
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Publication
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gja1 (Gja1 Rat)
    Tags
    monomerized superfolder GFP
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69007-plasmid-map-sequence-id-121423

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  19. OPRK1-Tango

    Plasmid
    #66462
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OPRK1 (OPRK1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    66462-plasmid-map-sequence-id-157905

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  20. MRGPRX2-Tango

    Plasmid
    #66440
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MRGPRX2 (MRGPRX2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 3881 - 3900 of 27466 results