27,469 results
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Plasmid#100087PurposeCatalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEzh2[SET] (Ezh2 Mouse)
UseCRISPRTags3XFLag-NLS-Ezh2[SET]-dCas9-NLSExpressionMammalianMutationEzh2[SET] includes aa 482-746PromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSNAP-CaaX Control Plasmid
Plasmid#101133PurposeExpression of SNAP-Tag and CaaX box (Farnesylation Signal) in Mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCaaX Box
TagsSNAP-tag (SNAP26m)ExpressionMammalianPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMOD_B2303
Plasmid#91068PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35SDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
UseCRISPRPromoterCmYLCVAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VAMP3-mCherry
Plasmid#92423PurposeExpression of VAMP3 C-terminally conjugated to mCherry.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVAMP3 (Vamp3 Mouse)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Plasmid#92390Purposeexpresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
ExpressionMammalianPromoterCMVAvailable sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MCS+
Plasmid#90412PurposeA FRET sensor that report the electric potential at the inner leaflet of cell plasma membraneDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLck10+ Venus+mcherry+ strech of positively charged amino acids
ExpressionMammalianAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX_TRC311 ETV5
Plasmid#74984PurposeConstitutive ETV5 ORF (codon optimized), blasticidin selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianMutationCodon optimized (synonymous)Available sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Dynamitin-GFP
Plasmid#51419Purposeexpression of GFP-tagged wild-type dynamitin p50 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDynamitin (DCTN2 Chicken)
TagsGFP and mycExpressionMammalianPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-1:PXN-EGFP
Plasmid#87420PurposeHomology arms and linker-EGFP sequence for C-terminus tagging of human PXNDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPXN Homology Arms with linker-EGFP (PXN Human)
UseCRISPR; Donor templateTagslinker-EGFPExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceMarch 15, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
Fireworks GFP[PTC-] (AVA2598)
Plasmid#85445PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PLAA
Plasmid#85669PurposeExpression of PLAA with N-terminal GFP tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPLAA (PLAA Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTriEx-mCherry-LOV2 I427T
Plasmid#81035PurposeMammalian expression of LOV I427T, a mutant with super short relaxation timeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLOV I427T
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationI427TAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRE-Tight-Rluc8
Plasmid#79844PurposeRluc8 reporter plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRluc8
UseSynthetic BiologyExpressionMammalianAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N3-TPC2
Plasmid#80153PurposeIntracellular Ca2+ store markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTPCN2 (TPCN2 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PhiC31-Neo-ins-5xTetO-pEF-H2B-Citrine-ins
Plasmid#78099PurposeNuclear fluorescent reporter in mammalian cells, phiC31 attB for integration, can be silenced with TetR-CR or rTetR-CR (e.g. CR can be KRAB or DNMT3B).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHistone H2B - Citrine (H2BC21 Synthetic, Human)
UseSynthetic BiologyExpressionMammalianPromoter5xTetO pEF alphaAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDestTol2pA2-9xGRE
Plasmid#74736PurposeGRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elementsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP
UseZebrafish expressionPromoternine GC-responsive elements (9xGRE) from the rat …Available sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHBV-S2-Luc
Plasmid#71417PurposeTo see HBV S2 promoter activity for luciferase assayDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHBV S2 promoter driving luciferase expression
UseLuciferaseExpressionMammalianPromoterHBV S2Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHBV-S1-Luc
Plasmid#71416PurposeTo see S1 promoter activity of HBV for luciferase assayDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHBV S1 promoter driving Luciferase expression
UseLuciferaseExpressionMammalianPromoterHBV S1Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 p53 S15D
Plasmid#69005Purposemammalian expression of human p53 S15D mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertp53 (TP53 Human)
ExpressionMammalianMutationS15DPromoterCMVAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GPR126-Tango
Plasmid#66314PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGPR126 (ADGRG6 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only