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30,870 results

Showing: 2121 - 2140 of 30870 results

  2. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

    Plasmid
    #196702
    Purpose
    Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.
    Depositor
    Karlene Cimprich
    Publication
    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human RNaseH1 wild type
    Expression
    Mammalian
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    196702-plasmid-map-sequence-id-385953

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  3. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185912-plasmid-map-sequence-id-367362

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  4. pX458M-53BP1-DN1S

    Plasmid
    #131045
    Purpose
    Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.
    Depositor
    Punam Malik
    Publication
    Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-53BP1-DN1S (TP53BP1 Human)
    Use
    CRISPR
    Tags
    GFP and SpCas9
    Expression
    Mammalian
    Mutation
    Only expressing residues 1231-1644 of complete 53…
    Promoter
    CBH
    Available since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    131045-plasmid-map-sequence-id-258265

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  5. pC1-SypHer3s-dmito

    Plasmid
    #108119
    Purpose
    Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.
    Depositor
    Vsevolod Belousov
    Publication
    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SypHer3s-dmito
    Tags
    ratiometric YFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108119-plasmid-map-sequence-id-207897

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  9. pTriEx4-H6-FGAm (FlincG3)

    Plasmid
    #49202
    Purpose
    Fluorescent reporter for cGMP
    Depositor
    John Garthwaite
    Publication
    Bhargava et al Front Mol Neurosci. 2013 Sep 24;6:26. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FlincG3
    Tags
    His tag + other tags
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Met to Lys in cpEGFP region similar to CGaMP3
    Promoter
    CMV
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49202-plasmid-map-sequence-id-72703

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  16. pTriEx-PA-Rac1

    Plasmid
    #22024
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Publication
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    Tags
    6X His
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 2121 - 2140 of 30870 results